FastQCFastQC Report
Fri 10 Feb 2017
SRR2031415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649006
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.12007233448513832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.050.541631
GTATCAA27050.043.3366971
ATCAACG32750.034.9352343
TCAACGC32850.034.8288844
CAACGCA33950.033.700415
AACGCAG35850.032.0360646
TATCAAC36700.031.3016822
GTACATG51450.028.9647881
TACATGG52400.027.992752
ACGCAGA40850.027.8825247
CGCAGAG41850.027.2162768
ACATGGG53300.026.8990943
CATGGGG38400.025.8388374
GCAGAGT51850.021.1441069
AGAGTAC44550.020.39894310-11
GTACTAG3353.45608E-1119.8339541
GCGCCGT1200.004788577619.7743266
CAGAGTA47350.018.79177710-11
TGTACGC1300.00759217818.2576562
GAGTACT23450.018.21319812-13
Produced by FastQC (version 0.11.5)