FastQCFastQC Report
Fri 10 Feb 2017
SRR2031415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649006
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.13080607347699158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16690.10121248800792719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.050.2552031
GTATCAA31250.046.702031
TCAACGC37350.038.5316284
ATCAACG37900.037.972463
CAACGCA38800.037.2140735
TATCAAC40300.036.1836052
AACGCAG40700.035.710216
ACGCAGA45350.031.9438977
CGCAGAG47050.030.5878058
GTACATG52350.029.3314271
TACATGG52850.027.4115332
ACATGGG55800.025.4508513
GCAGAGT57400.024.5759249
CATGGGG40200.022.3306354
GTATAGC6450.021.3739931
AGAGTAC48650.021.03922710-11
CAGAGTA52800.020.64496210-11
GAGTACT27250.019.78401612-13
GTATAAT10050.019.3939021
GTATAGG5100.018.6426321
Produced by FastQC (version 0.11.5)