FastQCFastQC Report
Fri 10 Feb 2017
SRR2031413_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031413_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7474
Sequences flagged as poor quality0
Sequence length101
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1822.435108375702435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1672.2344126304522343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1001.337971635001338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA791.056997591651057No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA410.5485683703505485No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA390.5218089376505218No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.5084292213005084No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT280.3746320578003747No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.34787262510034783No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG250.3344929087503345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.3077334760503077No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.29435375970029437No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT210.280974043350281No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA210.280974043350281No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC180.24083489430024083No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT170.22745517795022743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC160.21407546160021407No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA160.21407546160021407No Hit
GTACATGGGATCGTCAAGAAGTGCCTGGAGCTCTTCTCCGAGCTGGCTGA150.2006957452502007No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA150.2006957452502007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG140.18731602890018734No Hit
GGTATCAACGCAGAGAAGAAGCAGCTGTTGGTGTAGCACAAGTGGCAGAA140.18731602890018734No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA140.18731602890018734No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.17393631255017392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC120.16055659620016055No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC120.16055659620016055No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG120.16055659620016055No Hit
GTACTATTTCATAGTTGCTCTGAATTAAGGATTTCAGGTTTAACTGGTAG110.14717687985014719No Hit
ATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGG110.14717687985014719No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA110.14717687985014719No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG110.14717687985014719No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT110.14717687985014719No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG110.14717687985014719No Hit
GTCATATACACCACCTCGAAGCCCCGCTTCCGCACTCGCTCCACAAAGGC110.14717687985014719No Hit
CTATAGGGTTGAGGGTTTTAGACAATCTCTAGGAAAGGGATACAAAACAG100.1337971635001338No Hit
GTACATGGGATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCT100.1337971635001338No Hit
GAGTACATGGGCGGCCTTTTGTCCAGCTCAGCCTGGGATGTCTATAGGGT100.1337971635001338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC100.1337971635001338No Hit
ATCTACACCTATACTTAAAAATCATGAATCTGACTAGCCATGCCATTGAA100.1337971635001338No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT100.1337971635001338No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG100.1337971635001338No Hit
GTACATGGGTGCATATTAGATATGTCTAAGTCTGTAGCTGCTCCGAAAGA100.1337971635001338No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC90.12041744715012041No Hit
ATACAGAACCCCACTAGCAGCTTTGCGTAGTGACCGAGACATTTTTTTTT90.12041744715012041No Hit
GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12041744715012041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG90.12041744715012041No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.12041744715012041No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12041744715012041No Hit
GAACTACAGATTAAGAATCAGAAAGGGAAACTAATGTAGTATCAGCCAAT90.12041744715012041No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG90.12041744715012041No Hit
GAGTACATGGGATCGTCAAGAAGTGCCTGGAGCTCTTCTCCGAGCTGGCT80.10703773080010703No Hit
CTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGT80.10703773080010703No Hit
ACTTAGAGCTGCATCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAA80.10703773080010703No Hit
AACTAATGTAGTATCAGCCAATAGACTGGAATCATTTCTTCCCGAAGATG80.10703773080010703No Hit
GTACATGGGCGGCCTTTTGTCCAGCTCAGCCTGGGATGTCTATAGGGTTG80.10703773080010703No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG80.10703773080010703No Hit
CTATGAGGCCTTCTCCAAAAATTTAAAGCTTGGAATTCATGAAGATTCCA80.10703773080010703No Hit
CTTTTGTCCAGCTCAGCCTGGGATGTCTATAGGGTTGAGGGTTTTAGACA80.10703773080010703No Hit
TATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10703773080010703No Hit
GTCTAAGTCTGTAGCTGCTCCGAAAGATGTCAAACCAACTTTAATACCGA80.10703773080010703No Hit
GGTATCAACGCAGAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10703773080010703No Hit
GTATCAGCCAATAGACTGGAATCATTTCTTCCCGAAGATGCTAGGTGCAG80.10703773080010703No Hit
GTATCAACGCAGAGTACATGGGTGCATATTAGATATGTCTAAGTCTGTAG80.10703773080010703No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC80.10703773080010703No Hit
CTCTAGTACTGAGTGGTTTTCAATGGCATGGCTAGTCAGATTCATGATTT80.10703773080010703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTT901.70985E-1029.02777912-13
TACTTTT1005.838956E-1026.12514-15
GTACTTT951.00353645E-825.014-15
GAGTACT951.00353645E-825.012-13
ACTTTTT1157.621202E-820.65217416-17
AACGCAG3402.1827873E-1119.5588255
ACGCAGA3554.0017767E-1118.7323956
GTATCAA3052.8085196E-918.6885261
GCAGAGT3604.9112714E-1118.4722238
CGCAGAG3656.002665E-1118.219187
CAACGCA3707.4578566E-1117.9729734
TCAACGC3758.913048E-1117.7333343
TATCAAC3801.09139364E-1017.51
CAGAGTA3301.6370905E-1017.2727289
ATCAACG3851.3096724E-1017.2727262
AGAGTAC3050.017.1311510-11
GGTATCA2455.8969646E-515.5102051
GTACATG2550.001474998113.0392161
TACATGG2552.889876E-413.0392162
AGTACAT2258.150874E-512.6666662
Produced by FastQC (version 0.11.5)