FastQCFastQC Report
Fri 10 Feb 2017
SRR2031413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7474
Sequences flagged as poor quality0
Sequence length101
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT3454.616002140754616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2172.9033984479529034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1952.609044688252609No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1121.4985282312014987TruSeq Adapter, Index 10 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1121.4985282312014987No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG1121.4985282312014987TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT861.1506556061011506RNA PCR Primer, Index 10 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.9365801445009366No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.7358843992507359No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA520.6957452502006957No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA440.5887075194005887RNA PCR Primer, Index 10 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA270.3612523414503613No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT240.3211131924003211TruSeq Adapter, Index 10 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.3211131924003211No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT220.29435375970029437No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.29435375970029437No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG200.2675943270002676No Hit
GTACATGGGATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCT200.2675943270002676No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA190.2542146106502542No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT180.24083489430024083No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG180.24083489430024083No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA170.22745517795022743No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.21407546160021407No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG160.21407546160021407No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC160.21407546160021407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG150.2006957452502007No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA140.18731602890018734No Hit
GTACATGGGATCGTCAAGAAGTGCCTGGAGCTCTTCTCCGAGCTGGCTGA140.18731602890018734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG140.18731602890018734No Hit
GTCTAAGTCTGTAGCTGCTCCGAAAGATGTCAAACCAACTTTAATACCGA140.18731602890018734No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC140.18731602890018734No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC130.17393631255017392Illumina Paired End PCR Primer 2 (95% over 24bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT130.17393631255017392No Hit
CTATAGGGTTGAGGGTTTTAGACAATCTCTAGGAAAGGGATACAAAACAG130.17393631255017392No Hit
GTACATGGGAGGTCTAAATGTCTTCCCAAAAAATTGGTGTGTTTTTTGCC130.17393631255017392No Hit
GTACATGGGCGGCCTTTTGTCCAGCTCAGCCTGGGATGTCTATAGGGTTG130.17393631255017392No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.17393631255017392No Hit
GTACATGGGTGCATATTAGATATGTCTAAGTCTGTAGCTGCTCCGAAAGA130.17393631255017392No Hit
TCCCTATCCTGCCTATCCTGCCCCTCAACCTCTTCATTCCTAATTCCTCC120.16055659620016055No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA120.16055659620016055No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT120.16055659620016055No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG120.16055659620016055TruSeq Adapter, Index 10 (95% over 24bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA120.16055659620016055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG110.14717687985014719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG110.14717687985014719No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA110.14717687985014719No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.14717687985014719No Hit
GTCATATACACCACCTCGAAGCCCCGCTTCCGCACTCGCTCCACAAAGGC110.14717687985014719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC100.1337971635001338No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC100.1337971635001338No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA100.1337971635001338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.1337971635001338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC100.1337971635001338No Hit
GTCTTAATGTGGACCTTTTATTTTGTAAATAATTATGGAAATGATTGTGG100.1337971635001338No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT100.1337971635001338No Hit
GAGTACATGGGATCGTCAAGAAGTGCCTGGAGCTCTTCTCCGAGCTGGCT90.12041744715012041No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG90.12041744715012041No Hit
CTATGAAATAGTACCTTGGAGGAATTAGGAATGAAGAGGTTGAGGGGCAG90.12041744715012041No Hit
TATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12041744715012041No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12041744715012041No Hit
GTCAAGAAGTGCCTGGAGCTCTTCTCCGAGCTGGCTGAGGACAAGGAGAA90.12041744715012041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC90.12041744715012041No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT90.12041744715012041No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT90.12041744715012041No Hit
CATTAAGACATTGAACAGTAAAATATGTAATAAATGCTCCAATCTACACC80.10703773080010703No Hit
AAATATAACAAAGAACTACAGATTAAGAATCAGAAAGGGAAACTAATGTA80.10703773080010703No Hit
CTCTAGGAAAGGGATACAAAACAGATCCGATGGAAGAAACCTACTTCCCT80.10703773080010703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC80.10703773080010703No Hit
GTATATGACTGAGCCTATTGACGAGTACTGCGTGCAGCAGCTCAAGGAGT80.10703773080010703No Hit
GTGATATAGTAGATGGACTTCTGGGTCTCCTTCATGCGAGACACATACTC80.10703773080010703No Hit
CTATATCACTGGTGAGAGCAAAGAGCAAGTGGCCAACTCTGCCTTTGTGG80.10703773080010703No Hit
GGTATCAACGCAGAGTACATGGGATCGTCAAGAAGTGCCTGGAGCTCTTC80.10703773080010703No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA80.10703773080010703No Hit
CTATCACACCTCTCAGTCTGGAGATGAGATGACCTCCTTGTCAGAGTATG80.10703773080010703No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC80.10703773080010703No Hit
ATCTCTAGGAAAGGGATACAAAACAGATCCGATGGAAGAAACCTACTTCC80.10703773080010703No Hit
GGCTTATTGTTAATGCCTTATTTATAAAATGTTAAAACAAAATAGAATGT80.10703773080010703No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA80.10703773080010703No Hit
ACATATATTTATTCATAAAATTGCAATAAATTATAATAAAAAAGATGACA80.10703773080010703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT800.065.31255
TATACAC900.058.0555573
ATACACA900.058.0555574
CTTATAC950.055.01
ACACATC1000.052.256
CACATCT1050.049.7619067
TTATACA1050.049.7619062
ACATCTC1100.047.58
CATCTCC1201.8189894E-1243.5416689
GTACATG1702.561137E-927.9411771
TACATGG1702.561137E-927.9411772
ACATGGG1604.6864443E-826.7187523
GAGTACT1251.0913936E-1124.69999912-13
CATGGGA1201.4992642E-423.754
GTACTTT1353.092282E-1122.87036914-15
ATCTCCG1152.9576768E-922.71739210-11
AGTACTT1257.748895E-920.912-13
ACTTTTT1501.2551027E-1020.58333416-17
GTCTTCT1301.2176315E-820.09615548-49
GCTTGAA1301.2176315E-820.09615556-57
Produced by FastQC (version 0.11.5)