##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031413_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7474 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.847605030773344 34.0 33.0 34.0 31.0 34.0 2 32.973240567299975 34.0 33.0 34.0 31.0 34.0 3 32.93992507358844 34.0 33.0 34.0 31.0 34.0 4 36.277227722772274 37.0 37.0 37.0 35.0 37.0 5 36.26732673267327 37.0 37.0 37.0 35.0 37.0 6 36.41851752742842 37.0 37.0 37.0 35.0 37.0 7 36.432298635268936 37.0 37.0 37.0 35.0 37.0 8 36.44206582820444 37.0 37.0 37.0 35.0 37.0 9 38.257827134064755 39.0 39.0 39.0 37.0 39.0 10-11 38.236085094995985 39.0 39.0 39.0 37.0 39.0 12-13 38.22364195879047 39.0 39.0 39.0 37.0 39.0 14-15 39.488359646775486 41.0 39.0 41.0 36.5 41.0 16-17 39.45651592186246 41.0 39.0 41.0 36.5 41.0 18-19 39.7487958255285 41.0 40.0 41.0 37.0 41.0 20-21 39.80940594059406 41.0 40.0 41.0 37.0 41.0 22-23 39.69233342253144 41.0 40.0 41.0 35.5 41.0 24-25 39.57017661225582 41.0 40.0 41.0 35.0 41.0 26-27 39.240500401391486 41.0 39.0 41.0 35.0 41.0 28-29 38.9059405940594 41.0 38.0 41.0 35.0 41.0 30-31 38.33656676478459 40.0 37.0 41.0 35.0 41.0 32-33 37.595263580412094 40.0 35.0 41.0 34.5 41.0 34-35 37.156944072785656 40.0 35.0 41.0 33.0 41.0 36-37 36.7498662028365 40.0 35.0 41.0 33.0 41.0 38-39 36.09620016055659 39.0 35.0 41.0 31.0 41.0 40-41 35.66269735081616 39.0 35.0 41.0 28.5 41.0 42-43 35.03425207385604 38.5 35.0 41.0 22.5 41.0 44-45 34.242373561680495 38.0 35.0 41.0 14.0 41.0 46-47 33.14249397912764 37.0 33.5 41.0 9.0 41.0 48-49 31.72651859780573 36.5 31.0 40.5 7.0 41.0 50-51 30.716885202033716 35.0 25.0 40.0 7.0 41.0 52-53 30.515252876639014 35.5 24.0 40.5 7.0 41.0 54-55 30.244848809205244 35.0 22.5 40.0 7.0 41.0 56-57 29.894969226652396 35.0 20.0 40.5 7.0 41.0 58-59 29.414771206850414 35.0 18.0 40.0 7.0 41.0 60-61 28.821849076799573 35.0 15.0 40.0 7.0 41.0 62-63 28.32907412362858 35.0 13.5 39.0 7.0 41.0 64-65 27.86874498260637 35.0 10.0 39.0 7.0 41.0 66-67 27.13914905004014 35.0 10.0 37.5 7.0 41.0 68-69 26.390286325929893 35.0 10.0 37.0 7.0 41.0 70-71 25.631388814557134 34.5 9.0 36.5 7.0 39.5 72-73 24.983476050307733 34.0 8.0 36.0 7.0 39.0 74-75 24.66905271608242 34.0 8.0 35.5 7.0 39.0 76-77 23.87623762376238 32.0 8.0 35.0 7.0 37.0 78-79 24.191597538132193 33.0 7.0 35.0 7.0 37.0 80-81 24.129783248595132 33.0 7.0 35.0 7.0 36.5 82-83 24.033248595129784 33.0 7.0 35.0 7.0 36.0 84-85 23.81629649451432 33.0 7.0 35.0 7.0 36.0 86-87 23.62737489965213 33.0 7.0 35.0 7.0 36.0 88-89 23.51097136740701 33.0 7.0 35.0 7.0 35.0 90-91 23.37944875568638 33.0 7.0 35.0 7.0 35.0 92-93 23.312148782445814 32.0 7.0 35.0 7.0 35.0 94-95 23.39095531174739 33.0 7.0 35.0 7.0 35.0 96-97 23.354830077602355 32.0 7.0 35.0 7.0 35.0 98-99 23.27782981000803 32.0 7.0 35.0 5.0 35.0 100-101 23.020872357506022 31.5 7.0 35.0 5.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.08823222495097127 1101 2 -0.32476742007993664 1101 3 0.6296892632830122 1101 4 0.4150895979020959 1101 5 0.7762410621785492 1101 6 -0.3987094213656661 1101 7 0.22836379477004698 1101 8 0.2094958282458279 1101 9 0.8493025493025428 1101 10-11 0.48552447263386256 1101 12-13 0.3531982774170288 1101 14-15 -0.1315917444823782 1101 16-17 0.5907920493857972 1101 18-19 0.6718259894041338 1101 20-21 0.35558580597643896 1101 22-23 0.18176298527858847 1101 24-25 0.26707619637306124 1101 26-27 0.5303771315490167 1101 28-29 0.671455584346198 1101 30-31 -0.6355200933325875 1101 32-33 -0.9076424732674653 1101 34-35 -3.276445010429377 1101 36-37 -5.40894637769636 1101 38-39 -8.201571200789942 1101 40-41 -8.985527103105245 1101 42-43 -7.700446688727929 1101 44-45 -8.944697041962684 1101 46-47 -13.033692522755018 1101 48-49 -10.924369264994265 1101 50-51 -10.837158862549487 1101 52-53 -10.916374366374367 1101 54-55 -13.687151664886034 1101 56-57 -10.883425081862576 1101 58-59 -12.055575587606842 1101 60-61 -11.622905190873936 1101 62-63 -9.48461336117586 1101 64-65 -7.976948153120034 1101 66-67 -6.671070755055144 1101 68-69 -7.835950536731783 1101 70-71 -8.53870959769397 1101 72-73 -5.367578471875348 1101 74-75 -5.482761249167499 1101 76-77 -5.808077310421066 1101 78-79 -9.192921892921893 1101 80-81 -9.005412931975432 1101 82-83 -6.226181674228545 1101 84-85 -7.1858051092426045 1101 86-87 -8.174222854691616 1101 88-89 -8.624904392482511 1101 90-91 -6.0698871151996165 1101 92-93 -7.227574422105665 1101 94-95 -6.9003499972249855 1101 96-97 -7.479054047804034 1101 98-99 -6.499980979668475 1101 100-101 -7.540618712884335 1102 1 0.578434441715693 1102 2 0.5085659132533991 1102 3 0.9630225966163479 1102 4 0.7484229312354316 1102 5 0.7762410621785492 1102 6 0.6012905786343339 1102 7 0.5616971281033827 1102 8 0.5428291615791636 1102 9 0.8493025493025428 1102 10-11 0.5688578059671912 1102 12-13 0.8531982774170288 1102 14-15 0.7434082555176218 1102 16-17 1.3407920493857972 1102 18-19 1.1301593227374624 1102 20-21 0.8139191393097676 1102 22-23 -0.2765703480547401 1102 24-25 1.0587428630397326 1102 26-27 1.072043798215681 1102 28-29 1.8797889176795266 1102 30-31 0.9061465733340839 1102 32-33 1.4673575267325347 1102 34-35 0.9318883229039514 1102 36-37 1.8410536223036402 1102 38-39 3.090095465876722 1102 40-41 3.5561395635614232 1102 42-43 3.966219977938735 1102 44-45 4.180302958037316 1102 46-47 4.882974143911646 1102 48-49 -0.09103593166093304 1102 50-51 -0.6288255292161544 1102 52-53 -0.5830410330410309 1102 54-55 1.0211816684473014 1102 56-57 5.658241584804092 1102 58-59 2.0694244123931576 1102 60-61 1.3770948091260635 1102 62-63 1.7653866388241397 1102 64-65 7.689718513546634 1102 66-67 6.24559591161152 1102 68-69 4.997382796601553 1102 70-71 4.71129040230603 1102 72-73 8.007421528124652 1102 74-75 3.9339054174991652 1102 76-77 4.52525602291227 1102 78-79 4.890411440411441 1102 80-81 5.3279204013579005 1102 82-83 7.440484992438119 1102 84-85 5.8141948907573955 1102 86-87 5.3674438119750505 1102 88-89 7.416762274184155 1102 90-91 6.138446218133719 1102 92-93 5.897425577894335 1102 94-95 3.7663166694416823 1102 96-97 5.104279285529298 1102 98-99 8.20835235366486 1102 100-101 10.501047953782333 1103 1 -0.06442270114145288 1103 2 0.00856591325339906 1103 3 -0.17983454624079798 1103 4 -0.5372913544788531 1103 5 0.20481249074997976 1103 6 0.029862007205764485 1103 7 -0.009731443325186717 1103 8 0.2571148758648789 1103 9 -0.007840307840311311 1103 10-11 0.24742923453862176 1103 12-13 0.2817697059884594 1103 14-15 -0.3815917444823782 1103 16-17 -3.480636522042772 1103 18-19 -0.9591263915482529 1103 20-21 0.11749056788119816 1103 22-23 -0.15157034805474012 1103 24-25 0.36231429161116324 1103 26-27 0.26847236964425036 1103 28-29 -0.4952110823204734 1103 30-31 -0.3259962838087773 1103 32-33 0.7709289553039653 1103 34-35 1.1640311800468126 1103 36-37 1.876767908017932 1103 38-39 0.8579526087338607 1103 40-41 1.0382824207042844 1103 42-43 0.35907712079588094 1103 44-45 1.3767315294658857 1103 46-47 0.3115455724830767 1103 48-49 -0.26960736023236365 1103 50-51 1.567603042212415 1103 52-53 0.9526732526732538 1103 54-55 -1.1395326172669833 1103 56-57 -5.288186986624478 1103 58-59 -5.769861301892558 1103 60-61 -5.837190905159652 1103 62-63 -5.48461336117586 1103 64-65 -4.810281486453366 1103 66-67 -4.18297551695991 1103 68-69 -3.2169029176841626 1103 70-71 -3.3244238834082545 1103 72-73 -2.6532927575896323 1103 74-75 -3.5660945825008348 1103 76-77 -3.8676011199448723 1103 78-79 -3.359588559588559 1103 80-81 -3.6720795986420995 1103 82-83 -1.559515007561881 1103 84-85 -5.507233680671174 1103 86-87 -3.864699045167807 1103 88-89 -3.1725234401015605 1103 90-91 -5.486553781866281 1103 92-93 -3.1561458506770954 1103 94-95 -5.483683330558318 1103 96-97 -4.217149285899271 1103 98-99 -2.9880762177637124 1103 100-101 -4.873952046217667 1104 1 0.703434441715693 1104 2 0.6335659132533991 1104 3 0.08802259661634793 1104 4 0.24842293123543158 1104 5 0.5262410621785492 1104 6 0.6012905786343339 1104 7 0.5616971281033827 1104 8 0.5428291615791636 1104 9 0.4743025493025428 1104 10-11 0.6313578059671912 1104 12-13 -0.0843017225829712 1104 14-15 0.5559082555176218 1104 16-17 0.028292049385797213 1104 18-19 -0.11984067726253755 1104 20-21 -0.06108086069023244 1104 22-23 0.03592965194525988 1104 24-25 -0.44125713696026736 1104 26-27 -0.36545620178431903 1104 28-29 -2.1827110823204734 1104 30-31 -3.031353426665916 1104 32-33 -3.3451424732674653 1104 34-35 -3.3806116770960486 1104 36-37 -2.9714463776963598 1104 38-39 -2.722404534123278 1104 40-41 -4.068860436438577 1104 42-43 -2.846280022061265 1104 44-45 -4.507197041962684 1104 46-47 -4.742025856088354 1104 48-49 -6.028535931660933 1104 50-51 -4.128825529216154 1104 52-53 -7.708041033041031 1104 54-55 -9.791318331552699 1104 56-57 -8.029258415195908 1104 58-59 -7.8680755876068424 1104 60-61 -7.8104051908739365 1104 62-63 -6.35961336117586 1104 64-65 -3.9977814864533663 1104 66-67 -5.56690408838848 1104 68-69 -6.065117203398447 1104 70-71 -5.66370959769397 1104 72-73 -4.242578471875348 1104 74-75 -3.7535945825008348 1104 76-77 -4.97474397708773 1104 78-79 -7.172088559588559 1104 80-81 -3.4845795986420995 1104 82-83 -4.559515007561881 1104 84-85 -6.1858051092426045 1104 86-87 -5.5700561880249495 1104 88-89 -2.458237725815845 1104 90-91 -4.861553781866281 1104 92-93 -4.977574422105665 1104 94-95 -4.421183330558318 1104 96-97 -4.583220714470702 1104 98-99 -4.041647646335139 1104 100-101 -5.248952046217667 1105 1 0.6784344417156944 1105 2 -0.19143408674660378 1105 3 -0.23697740338365492 1105 4 0.348422931235433 1105 5 0.7762410621785492 1105 6 0.2012905786343353 1105 7 0.5616971281033827 1105 8 0.5428291615791636 1105 9 0.6493025493025399 1105 10-11 0.6188578059671883 1105 12-13 0.7531982774170274 1105 14-15 -0.581591744482381 1105 16-17 0.09079204938579721 1105 18-19 0.9551593227374653 1105 20-21 0.6889191393097676 1105 22-23 0.7484296519452656 1105 24-25 0.8337428630397383 1105 26-27 0.7970437982156753 1105 28-29 0.30478891767952376 1105 30-31 0.031146573334083882 1105 32-33 0.3423575267325347 1105 34-35 0.9068883229039528 1105 36-37 0.5910536223036402 1105 38-39 1.215095465876722 1105 40-41 2.2811395635614247 1105 42-43 2.016219977938732 1105 44-45 3.505302958037319 1105 46-47 2.4829741439116404 1105 48-49 0.2589640683390684 1105 50-51 0.8961744707838513 1105 52-53 -0.33304103304103094 1105 54-55 -2.9538183315527 1105 56-57 -3.7167584151959083 1105 58-59 -3.855575587606843 1105 60-61 0.8770948091260635 1105 62-63 0.9153866388241383 1105 64-65 -1.8102814864533663 1105 66-67 -2.5544040883884804 1105 68-69 0.5973827966015541 1105 70-71 1.8612904023060288 1105 72-73 3.7324215281246538 1105 74-75 3.9339054174991652 1105 76-77 -1.6247439770877286 1105 78-79 0.3404114404114402 1105 80-81 -0.5720795986420981 1105 82-83 -1.259515007561884 1105 84-85 -0.9358051092426045 1105 86-87 -0.10755618802495093 1105 88-89 0.1417622741841562 1105 90-91 3.81344621813372 1105 92-93 -0.6275744221056634 1105 94-95 0.11631666944168373 1105 96-97 0.4542792855292994 1105 98-99 2.3833523536648578 1105 100-101 1.5260479537823315 1106 1 0.3641487274299777 1106 2 -0.13429122960374684 1106 3 -0.4655488319550827 1106 4 -0.3944342116217143 1106 5 -0.08090179496430494 1106 6 0.029862007205764485 1106 7 0.4188399852462368 1106 8 0.5428291615791636 1106 9 0.8493025493025428 1106 10-11 0.8188578059671912 1106 12-13 0.8531982774170288 1106 14-15 1.6184082555176218 1106 16-17 1.376506335100082 1106 18-19 1.1123021798803165 1106 20-21 0.6889191393097676 1106 22-23 -0.08014177662617072 1106 24-25 -0.42339999410312856 1106 26-27 -0.44581334464146494 1106 28-29 0.1476460605366725 1106 30-31 0.6740037161912298 1106 32-33 -0.5147853304103265 1106 34-35 1.235459751475382 1106 36-37 -0.9803749491249292 1106 38-39 -5.4277616769804204 1106 40-41 -4.961717579295719 1106 42-43 -4.426637164918407 1106 44-45 -4.123268470534114 1106 46-47 -4.117025856088354 1106 48-49 -2.6981787888037907 1106 50-51 -1.7895398149304391 1106 52-53 -1.6901838901838886 1106 54-55 -1.568104045838414 1106 56-57 -1.2881869866244777 1106 58-59 -0.6270041590354118 1106 60-61 0.9485233805546365 1106 62-63 -4.98461336117586 1106 64-65 -7.881710057881936 1106 66-67 -7.040118374102764 1106 68-69 -6.502617203398447 1106 70-71 -5.681566740551112 1106 72-73 -4.153292757589632 1106 74-75 -4.423237439643692 1106 76-77 -2.7961725485162994 1106 78-79 -0.43101713101712846 1106 80-81 -2.8149367414992454 1106 82-83 -4.988086436133312 1106 84-85 -2.221519394956889 1106 86-87 -5.364699045167804 1106 88-89 -5.101094868672988 1106 90-91 -2.700839496151996 1106 92-93 -5.656145850677095 1106 94-95 -4.983683330558318 1106 96-97 -4.574292143042133 1106 98-99 -5.273790503477997 1106 100-101 -3.5168091890748094 1107 1 -0.046565558284306974 1107 2 -0.24143408674660094 1107 3 0.4630225966163479 1107 4 0.7484229312354316 1107 5 -0.5987589378214508 1107 6 0.10129057863433388 1107 7 0.3116971281033827 1107 8 0.4178291615791636 1107 9 0.5993025493025428 1107 10-11 -0.36864219403280885 1107 12-13 0.4781982774170288 1107 14-15 0.8684082555176218 1107 16-17 0.8407920493857972 1107 18-19 0.5051593227374624 1107 20-21 0.6889191393097676 1107 22-23 1.0359296519452599 1107 24-25 1.3087428630397326 1107 26-27 1.447043798215681 1107 28-29 1.1297889176795266 1107 30-31 0.7186465733340839 1107 32-33 1.5923575267325347 1107 34-35 2.3693883229039514 1107 36-37 2.5910536223036402 1107 38-39 1.402595465876722 1107 40-41 2.1811395635614232 1107 42-43 3.403719977938735 1107 44-45 3.8678029580373163 1107 46-47 3.132974143911646 1107 48-49 5.033964068339067 1107 50-51 5.621174470783846 1107 52-53 5.666958966958969 1107 54-55 6.208681668447301 1107 56-57 5.783241584804092 1107 58-59 4.9444244123931576 1107 60-61 3.9395948091260635 1107 62-63 4.14038663882414 1107 64-65 5.064718513546634 1107 66-67 5.87059591161152 1107 68-69 4.434882796601553 1107 70-71 3.3987904023060302 1107 72-73 0.8199215281246524 1107 74-75 2.1839054174991652 1107 76-77 2.90025602291227 1107 78-79 -0.23458855958855906 1107 80-81 2.5154204013579005 1107 82-83 3.377984992438119 1107 84-85 2.8141948907573955 1107 86-87 3.3674438119750505 1107 88-89 2.666762274184155 1107 90-91 2.388446218133719 1107 92-93 0.5849255778943352 1107 94-95 2.2663166694416823 1107 96-97 0.666779285529298 1107 98-99 1.0208523536648606 1107 100-101 -0.06145204621766709 1108 1 0.07843444171569303 1108 2 0.5799944846819685 1108 3 -0.036977403383652074 1108 4 0.17699435980686218 1108 5 -2.7951875092500202 1108 6 -0.11299513565138142 1108 7 -0.009731443325186717 1108 8 0.11425773300773301 1108 9 0.8493025493025428 1108 10-11 0.39028637739576055 1108 12-13 0.1389125631313135 1108 14-15 0.975551112660483 1108 16-17 0.3050777636715125 1108 18-19 0.8265878941660318 1108 20-21 0.9746334250240523 1108 22-23 1.205572509088114 1108 24-25 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0.5616971281033827 2312 8 0.2571148758648789 2312 9 0.8493025493025428 2312 10-11 0.8188578059671912 2312 12-13 0.7817697059884594 2312 14-15 0.83269396980333 2312 16-17 1.1622206208143666 2312 18-19 1.1123021798803236 2312 20-21 1.1889191393097676 2312 22-23 1.3484296519452599 2312 24-25 1.2908857201825867 2312 26-27 1.5541866553585422 2312 28-29 1.8619317748223878 2312 30-31 1.0311465733340839 2312 32-33 1.699500383875396 2312 34-35 2.0211740371896667 2312 36-37 0.9481964794465014 2312 38-39 -2.7134759626947087 2312 40-41 -6.247431865010007 2312 42-43 -1.283780022061265 2312 44-45 -0.5518398991055449 2312 46-47 1.0258312867687849 2312 48-49 3.516106925481928 2312 50-51 -1.0038255292161544 2312 52-53 -1.1187553187553156 2312 54-55 1.431895954161586 2312 56-57 3.2118130133755223 2312 58-59 1.8729958409645882 2312 60-61 1.2342376662689212 2312 62-63 1.9439580673955703 2312 64-65 3.761147084975203 2312 66-67 0.8884530544686626 2312 68-69 -0.788331489112732 2312 70-71 -0.1815667405511121 2312 72-73 0.918135813838937 2312 74-75 -1.4946660110722654 2312 76-77 -0.15331540565916058 2312 78-79 -0.7167314167314167 2312 80-81 -0.3149367414992419 2312 82-83 0.2261992781524036 2312 84-85 2.421337747900253 2312 86-87 -0.3646990451678036 2312 88-89 3.8274765598984395 2312 90-91 3.799160503848004 2312 92-93 2.55813986360862 2312 94-95 0.9448880980131129 2312 96-97 -0.9314350001849867 2312 98-99 1.4404952108077183 2312 100-101 -0.3025234747890977 2313 1 0.07843444171569303 2313 2 -0.17325226856478082 2313 3 -0.7642501306563787 2313 4 -0.6152134324009353 2313 5 0.04896833490582253 2313 6 0.3285633059070605 2313 7 0.19806076446701582 2313 8 0.17919279794279674 2313 9 -0.3325156325156371 2313 10-11 -1.7720512849419023 2313 12-13 0.12592555014430218 2313 14-15 0.2093173464267153 2313 16-17 -0.04557158697783592 2313 18-19 -0.1993861318079908 2313 20-21 -0.31108086069023244 2313 22-23 -0.5152067116910999 2313 24-25 -0.33898440968754073 2313 26-27 -0.802956201784319 2313 28-29 -1.1315747186841136 2313 30-31 -0.6052170630295564 2313 32-33 -0.43036974599473865 2313 34-35 -0.46583894982332197 2313 36-37 -0.9544009231509065 2313 38-39 -3.784904534123278 2313 40-41 -2.8643149818931235 2313 42-43 -2.465598203879452 2313 44-45 -1.2856061328717772 2313 46-47 -4.389753128815627 2313 48-49 -6.022854113479113 2313 50-51 -5.049280074670701 2313 52-53 -5.1512228512228475 2313 54-55 -5.444727422461792 2313 56-57 -4.898576597014088 2313 58-59 -4.828302860334116 2313 60-61 -5.57745064541939 2313 62-63 -5.257340633903134 2313 64-65 -5.67391785008973 2313 66-67 -4.34531317929757 2313 68-69 -2.7753444761257207 2313 70-71 -2.5841641431485165 2313 72-73 -3.4584875627844376 2313 74-75 -1.1570036734099283 2313 76-77 -2.31565306799682 2313 78-79 -2.041406741406739 2313 80-81 -2.7629886895511895 2313 82-83 -1.6504240984709746 2313 84-85 -0.9812596546971513 2313 86-87 -2.0075561880249495 2313 88-89 -3.3218740894522085 2313 90-91 -1.895644690957191 2313 92-93 -1.4548471493783914 2313 94-95 0.24358939671440893 2313 96-97 -1.4639025326525221 2313 98-99 -2.143920373607866 2313 100-101 -0.10122477349039372 2314 1 -1.3761110128297602 2314 2 -0.9914340867466009 2314 3 -1.4915228579291053 2314 4 -0.2515770687645684 2314 5 -1.4055771196396307 2314 6 -1.3078003304565726 2314 7 -1.2564846900784374 2314 8 -1.2753526566026565 2314 9 -1.8779701779701838 2314 10-11 -0.9538694667600822 2314 12-13 0.39865282287157555 2314 14-15 1.072953710063075 2314 16-17 1.3635193221130706 2314 18-19 0.8915229591011027 2314 20-21 0.9616464120370409 2314 22-23 0.9393387428543534 2314 24-25 0.6155610448579196 2314 26-27 0.8334074345793212 2314 28-29 0.1866070994977136 2314 30-31 0.9402374824249904 2314 32-33 1.5241757085507217 2314 34-35 2.215979231994858 2314 36-37 2.7274172586672734 2314 38-39 3.0787318295130888 2314 40-41 3.544775927197783 2314 42-43 4.3071290688478285 2314 44-45 1.9871211398555033 2314 46-47 2.6557014166389195 2314 48-49 4.0680549774299735 2314 50-51 4.768901743511119 2314 52-53 5.166958966958969 2314 54-55 5.737090759356395 2314 56-57 5.510514312076818 2314 58-59 6.4444244123931576 2314 60-61 7.149822081853337 2314 62-63 8.697204820642327 2314 64-65 7.0533548771829935 2314 66-67 7.24559591161152 2314 68-69 5.67920097841974 2314 70-71 6.506744947760577 2314 72-73 6.541512437215559 2314 74-75 6.161178144771892 2314 76-77 7.047983295639543 2314 78-79 6.276775076775078 2314 80-81 5.8279204013579005 2314 82-83 5.985939537892666 2314 84-85 5.609649436211942 2314 86-87 6.4015347210659606 2314 88-89 6.223580456002335 2314 90-91 6.195264399951899 2314 92-93 6.363334668803429 2314 94-95 5.743589396714409 2314 96-97 6.354279285529298 2314 98-99 5.2651705354830405 2314 100-101 6.307866135600513 2315 1 0.41176777504902873 2315 2 0.00856591325339906 2315 3 -0.5925329589392092 2315 4 0.08175626456876728 2315 5 0.3317966177341063 2315 6 -0.3987094213656661 2315 7 0.11725268365893982 2315 8 0.09838471713472074 2315 9 0.4048581048580999 2315 10-11 0.09663558374496972 2315 12-13 0.01986494408370021 2315 14-15 -0.7704806333712639 2315 16-17 -0.13143017283642422 2315 18-19 -0.41150734392920896 2315 20-21 -0.644414194023561 2315 22-23 -0.8182370147214115 2315 24-25 -1.455146025849153 2315 26-27 -0.4140673128954333 2315 28-29 -1.2174333045426948 2315 30-31 -0.5244089822214733 2315 32-33 0.06457974895475616 2315 34-35 0.25133276734839427 2315 36-37 -0.24227971102968837 2315 38-39 -2.618237867456614 2315 40-41 -4.207749325327466 2315 42-43 -4.894891133172376 2315 44-45 -4.3613637086293515 2315 46-47 -4.783692522755018 2315 48-49 -5.785480376105376 2315 50-51 -5.948269973660597 2315 52-53 -5.1108188108188095 2315 54-55 -2.7982627759971415 2315 56-57 -1.550091748529244 2315 58-59 -3.5000200320512853 2315 60-61 -2.5673496353183793 2315 62-63 -2.929057805620303 2315 64-65 -1.3102814864533663 2315 66-67 -1.143292977277369 2315 68-69 -2.280394981176226 2315 70-71 -3.4831540421384126 2315 72-73 -6.423134027430905 2315 74-75 -6.066094582500835 2315 76-77 -4.669188421532173 2315 78-79 -3.1373663373663376 2315 80-81 -4.560968487530989 2315 82-83 -5.226181674228549 2315 84-85 -4.158027331464826 2315 86-87 -4.174222854691614 2315 88-89 -3.458237725815845 2315 90-91 -0.4309982263107237 2315 92-93 -2.227574422105665 2315 94-95 -3.5947944416694284 2315 96-97 -1.2012762700262591 2315 98-99 -2.02775875744625 2315 100-101 -4.707285379551003 2316 1 -0.5882322249509713 2316 2 0.09189924658673476 2316 3 -0.12031073671698778 2316 4 -0.08491040209790413 2316 5 -0.30709227115478654 2316 6 0.10129057863433388 2316 7 0.06169712810338268 2316 8 -0.3738375050875007 2316 9 -0.7340307840307929 2316 10-11 0.27719113930051975 2316 12-13 -0.4801350559162998 2316 14-15 -2.2982584111490425 2316 16-17 -1.5342079506142028 2316 18-19 -0.7865073439292019 2316 20-21 -0.5194141940235681 2316 22-23 -1.1515703480547401 2316 24-25 -2.4829238036269388 2316 26-27 -3.5946228684509904 2316 28-29 -1.7452110823204734 2316 30-31 -0.5521867599992518 2316 32-33 -1.6993091399341367 2316 34-35 -1.5681116770960486 2316 36-37 -1.2839463776963598 2316 38-39 -0.4099045341232781 2316 40-41 -0.8605271031052482 2316 42-43 -0.40878002206126496 2316 44-45 -0.3613637086293551 2316 46-47 0.9246408105783104 2316 48-49 1.2422974016724027 2316 50-51 3.3711744707838456 2316 52-53 3.541958966958969 2316 54-55 1.2711816684473014 2316 56-57 1.1999082514707595 2316 58-59 1.2777577457264933 2316 60-61 2.1270948091260635 2316 62-63 1.7237199721574754 2316 64-65 3.106385180213298 2316 66-67 2.3289292449448524 2316 68-69 0.5807161299348884 2316 70-71 1.9196237356393624 2316 72-73 1.6324215281246524 2316 74-75 1.6839054174991652 2316 76-77 0.8585893562456057 2316 78-79 0.8487447737447766 2316 80-81 1.3695870680245683 2316 82-83 1.440484992438119 2316 84-85 0.3558615574240598 2316 86-87 0.7007771453083862 2316 88-89 2.291762274184155 2316 90-91 2.1801128848003835 2316 92-93 2.1890922445609995 2316 94-95 2.849650002775018 2316 96-97 0.6876126188626301 2316 98-99 0.6250190203315285 2316 100-101 1.001047953782333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 4.0 15 12.0 16 47.0 17 97.0 18 153.0 19 191.0 20 273.0 21 388.0 22 392.0 23 266.0 24 254.0 25 251.0 26 205.0 27 219.0 28 312.0 29 236.0 30 79.0 31 70.0 32 84.0 33 79.0 34 128.0 35 324.0 36 440.0 37 1179.0 38 1396.0 39 394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.46216686755055 18.762555243069507 15.146645239051828 18.62863265032811 2 16.002140754616 22.103291410222102 42.4939791276425 19.4005887075194 3 17.674605298367673 34.41263045223441 35.28231201498529 12.630452234412632 4 9.325662295959326 25.16724645437517 31.455713138881457 34.05137811078405 5 8.683435911158684 40.68771742039069 33.02113995183302 17.607706716617606 6 21.059673534921057 39.08215145838908 18.116135937918116 21.742039068771742 7 17.875301043617874 42.052448488092054 18.771742039068773 21.300508429221303 8 26.264383195076263 33.74364463473374 20.2970297029703 19.694942467219693 9 27.26786192132727 13.834626705913836 18.102756221568104 40.79475515119079 10-11 22.350816162697352 30.144500936580144 23.22049772544822 24.284185175274285 12-13 28.3984479529034 29.4019266791544 24.357773615199356 17.84185175274284 14-15 25.68905539202569 27.435108375702434 26.67915440192668 20.1966818303452 16-17 17.48728926946749 33.823922932833824 28.719561145303718 19.96922665239497 18-19 18.223173668718225 25.28097404335028 36.11854428686112 20.377308001070375 20-21 19.28017126036928 25.16724645437517 36.51324591918652 19.03933636606904 22-23 19.046026224244045 27.736151993577735 35.79074123628579 17.427080545892426 24-25 18.81188118811881 28.906877174203906 36.994915707786994 15.286325929890287 26-27 19.454107572919455 28.264650789403266 36.734011238961735 15.547230398715547 28-29 15.647578271340649 26.21086432967621 38.74765854963875 19.393898849344392 30-31 18.932298635268932 28.405137811078408 37.83114797966283 14.831415573989831 32-33 19.82873963071983 27.26786192132727 40.32646507894032 12.576933369012577 34-35 12.97832485951298 28.144233342253145 42.01899919721701 16.85844260101686 36-37 17.547497993042548 29.181161359379182 36.47310677013647 16.7982338774418 38-39 16.30987423066631 26.806261707251807 38.781107840513776 18.102756221568104 40-41 13.901525287663901 28.043885469628044 40.092320042815096 17.962269199892962 42-43 16.343323521541343 28.244581214878245 39.242708054589244 16.16938720899117 44-45 15.480331816965482 29.763179020604763 38.35295691731335 16.403532245116402 46-47 18.16965480331817 27.067166176077066 35.248862724110246 19.514316296494513 48-49 17.44046026224244 26.351351351351347 34.92774953170993 21.28043885469628 50-51 15.56061011506556 28.565694407278563 34.43938988493444 21.434305592721433 52-53 15.33984479529034 30.35857639818036 34.59325662295959 19.708322183569706 54-55 15.326465078940327 29.816697886004818 31.602890018731607 23.253947016323252 56-57 17.982338774417983 29.856837035054856 27.515386673802517 24.645437516724645 58-59 15.286325929890287 31.415573989831415 31.89055392025689 21.407546160021408 60-61 17.68798501471769 32.94086165373294 27.281241637677283 22.08991169387209 62-63 13.613861386138614 35.309071447685305 28.01043617875301 23.066630987423068 64-65 14.724377843189723 34.30559272143431 28.538934974578538 22.431094460797432 66-67 15.293015788065292 34.285523146909284 27.60904468825261 22.812416376772813 68-69 15.928552314690927 33.03451966818303 28.07064490232807 22.966283114797967 70-71 15.714476853090714 32.11800909820712 28.91356703237891 23.253947016323252 72-73 16.276424939791276 30.4990634198555 29.83676746052984 23.38774417982339 74-75 16.30987423066631 30.057532780305056 29.6026224244046 24.02997056462403 76-77 16.256355365266256 29.830077602354834 29.950495049504948 23.963071982873963 78-79 16.477120685041477 28.77976986887878 30.438854696280437 24.304254749799302 80-81 16.276424939791276 29.455445544554454 29.83676746052984 24.43136205512443 82-83 17.741503880117744 28.866738025153865 28.07064490232807 25.32111319240032 84-85 17.326732673267326 28.09071447685309 28.318169654803317 26.264383195076263 86-87 18.203104094193204 28.538934974578538 26.71260369280171 26.545357238426547 88-89 18.73160289001873 26.505218089376502 26.58549638747659 28.177682633128175 90-91 19.634733743644635 26.79288199090179 25.749264115600752 27.823120149852826 92-93 18.484078137543484 25.695745250200698 27.30800107037731 28.51217554187851 94-95 19.367139416644367 25.227455177950226 26.705913834626706 28.6994915707787 96-97 19.025956649719024 25.836232271875836 26.391490500401392 28.746320578003747 98-99 20.397377575595396 26.056997591651054 25.541878512175543 28.003746320578003 100-101 20.404067433770404 26.0971367407011 24.886272411024887 28.61252341450361 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 94.0 1 55.0 2 20.0 3 17.5 4 7.5 5 8.5 6 16.5 7 29.0 8 41.0 9 41.0 10 27.5 11 15.5 12 12.0 13 12.5 14 13.0 15 13.5 16 18.5 17 17.5 18 16.5 19 17.5 20 17.0 21 20.5 22 21.5 23 20.0 24 39.5 25 57.5 26 61.5 27 82.0 28 102.5 29 122.5 30 144.0 31 163.5 32 198.0 33 236.0 34 233.5 35 229.5 36 242.0 37 246.5 38 234.0 39 248.5 40 274.0 41 234.0 42 205.0 43 212.0 44 216.5 45 267.0 46 302.0 47 273.5 48 292.5 49 296.0 50 338.0 51 320.0 52 211.5 53 215.0 54 182.5 55 115.5 56 86.5 57 57.0 58 50.5 59 45.0 60 34.0 61 26.0 62 17.0 63 10.0 64 4.5 65 3.0 66 2.5 67 2.0 68 1.5 69 2.0 70 2.0 71 1.5 72 0.5 73 2.0 74 2.0 75 0.5 76 0.0 77 0.5 78 0.5 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09365801445009367 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7474.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.94005887075194 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21174652241113 41.14262777629114 2 10.35548686244204 10.757291945410758 3 3.683668212261721 5.73989831415574 4 1.90623390005152 3.9603960396039604 5 1.3395157135497167 3.4787262510034784 6 0.7727975270479135 2.4083489430024083 7 0.6955177743431221 2.5287663901525286 8 0.38639876352395675 1.6055659620016056 9 0.23183925811437403 1.0837570243510837 >10 1.1591962905718702 9.151725983409152 >50 0.10303967027305513 3.518865400053519 >100 0.1545595054095827 14.624029970564626 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 345 4.616002140754616 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 2.9033984479529034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 195 2.609044688252609 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 112 1.4985282312014987 TruSeq Adapter, Index 10 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 1.4985282312014987 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 112 1.4985282312014987 TruSeq Adapter, Index 10 (96% over 28bp) ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 86 1.1506556061011506 RNA PCR Primer, Index 10 (95% over 23bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 70 0.9365801445009366 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.7358843992507359 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 52 0.6957452502006957 No Hit CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA 44 0.5887075194005887 RNA PCR Primer, Index 10 (96% over 28bp) GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 27 0.3612523414503613 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 24 0.3211131924003211 TruSeq Adapter, Index 10 (96% over 26bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.3211131924003211 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 22 0.29435375970029437 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.29435375970029437 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 20 0.2675943270002676 No Hit GTACATGGGATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCT 20 0.2675943270002676 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 19 0.2542146106502542 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18 0.24083489430024083 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 18 0.24083489430024083 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.22745517795022743 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.21407546160021407 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 16 0.21407546160021407 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 16 0.21407546160021407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 15 0.2006957452502007 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 14 0.18731602890018734 No Hit GTACATGGGATCGTCAAGAAGTGCCTGGAGCTCTTCTCCGAGCTGGCTGA 14 0.18731602890018734 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 14 0.18731602890018734 No Hit GTCTAAGTCTGTAGCTGCTCCGAAAGATGTCAAACCAACTTTAATACCGA 14 0.18731602890018734 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 14 0.18731602890018734 No Hit ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC 13 0.17393631255017392 Illumina Paired End PCR Primer 2 (95% over 24bp) GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13 0.17393631255017392 No Hit CTATAGGGTTGAGGGTTTTAGACAATCTCTAGGAAAGGGATACAAAACAG 13 0.17393631255017392 No Hit GTACATGGGAGGTCTAAATGTCTTCCCAAAAAATTGGTGTGTTTTTTGCC 13 0.17393631255017392 No Hit GTACATGGGCGGCCTTTTGTCCAGCTCAGCCTGGGATGTCTATAGGGTTG 13 0.17393631255017392 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.17393631255017392 No Hit GTACATGGGTGCATATTAGATATGTCTAAGTCTGTAGCTGCTCCGAAAGA 13 0.17393631255017392 No Hit TCCCTATCCTGCCTATCCTGCCCCTCAACCTCTTCATTCCTAATTCCTCC 12 0.16055659620016055 No Hit CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA 12 0.16055659620016055 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12 0.16055659620016055 No Hit CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG 12 0.16055659620016055 TruSeq Adapter, Index 10 (95% over 24bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 12 0.16055659620016055 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 11 0.14717687985014719 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 11 0.14717687985014719 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 11 0.14717687985014719 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.14717687985014719 No Hit GTCATATACACCACCTCGAAGCCCCGCTTCCGCACTCGCTCCACAAAGGC 11 0.14717687985014719 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 10 0.1337971635001338 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 10 0.1337971635001338 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 10 0.1337971635001338 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.1337971635001338 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 10 0.1337971635001338 No Hit GTCTTAATGTGGACCTTTTATTTTGTAAATAATTATGGAAATGATTGTGG 10 0.1337971635001338 No Hit TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT 10 0.1337971635001338 No Hit GAGTACATGGGATCGTCAAGAAGTGCCTGGAGCTCTTCTCCGAGCTGGCT 9 0.12041744715012041 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9 0.12041744715012041 No Hit CTATGAAATAGTACCTTGGAGGAATTAGGAATGAAGAGGTTGAGGGGCAG 9 0.12041744715012041 No Hit TATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.12041744715012041 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.12041744715012041 No Hit GTCAAGAAGTGCCTGGAGCTCTTCTCCGAGCTGGCTGAGGACAAGGAGAA 9 0.12041744715012041 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 9 0.12041744715012041 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9 0.12041744715012041 No Hit TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT 9 0.12041744715012041 No Hit CATTAAGACATTGAACAGTAAAATATGTAATAAATGCTCCAATCTACACC 8 0.10703773080010703 No Hit AAATATAACAAAGAACTACAGATTAAGAATCAGAAAGGGAAACTAATGTA 8 0.10703773080010703 No Hit CTCTAGGAAAGGGATACAAAACAGATCCGATGGAAGAAACCTACTTCCCT 8 0.10703773080010703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 8 0.10703773080010703 No Hit GTATATGACTGAGCCTATTGACGAGTACTGCGTGCAGCAGCTCAAGGAGT 8 0.10703773080010703 No Hit GTGATATAGTAGATGGACTTCTGGGTCTCCTTCATGCGAGACACATACTC 8 0.10703773080010703 No Hit CTATATCACTGGTGAGAGCAAAGAGCAAGTGGCCAACTCTGCCTTTGTGG 8 0.10703773080010703 No Hit GGTATCAACGCAGAGTACATGGGATCGTCAAGAAGTGCCTGGAGCTCTTC 8 0.10703773080010703 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8 0.10703773080010703 No Hit CTATCACACCTCTCAGTCTGGAGATGAGATGACCTCCTTGTCAGAGTATG 8 0.10703773080010703 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC 8 0.10703773080010703 No Hit ATCTCTAGGAAAGGGATACAAAACAGATCCGATGGAAGAAACCTACTTCC 8 0.10703773080010703 No Hit GGCTTATTGTTAATGCCTTATTTATAAAATGTTAAAACAAAATAGAATGT 8 0.10703773080010703 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 8 0.10703773080010703 No Hit ACATATATTTATTCATAAAATTGCAATAAATTATAATAAAAAAGATGACA 8 0.10703773080010703 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.01337971635001338 0.0 3 0.0 0.0 0.0 0.01337971635001338 0.0 4 0.0 0.0 0.0 0.01337971635001338 0.0 5 0.0 0.0 0.0 0.01337971635001338 0.0 6 0.0 0.0 0.0 0.01337971635001338 0.0 7 0.0 0.0 0.0 0.01337971635001338 0.0 8 0.0 0.0 0.0 0.01337971635001338 0.0 9 0.0 0.0 0.0 0.01337971635001338 0.0 10-11 0.0 0.0 0.0 0.01337971635001338 0.0 12-13 0.0 0.0 0.0 0.01337971635001338 0.0 14-15 0.0 0.0 0.0 0.01337971635001338 0.0 16-17 0.0 0.0 0.0 0.01337971635001338 0.0 18-19 0.0 0.0 0.0 0.01337971635001338 0.0 20-21 0.0 0.0 0.0 0.01337971635001338 0.0 22-23 0.0 0.0 0.0 0.060208723575060207 0.0 24-25 0.0 0.0 0.0 0.1404870216751405 0.0 26-27 0.0 0.0 0.0 0.2073856034252074 0.0 28-29 0.0 0.0 0.0 0.38132191597538134 0.0 30-31 0.0 0.0 0.0 0.6154669521006155 0.0 32-33 0.0 0.0 0.0 0.8094728391758095 0.0 34-35 0.0 0.0 0.0 1.1305860315761307 0.0 36-37 0.0 0.0 0.0 1.6925341182766926 0.0 38-39 0.0 0.0 0.0 2.241102488627241 0.0 40-41 0.0 0.0 0.0 2.742841851752743 0.0 42-43 0.0 0.0 0.0 2.9502274551779504 0.0 44-45 0.0 0.0 0.0 3.1442333422531443 0.0 46-47 0.0 0.0 0.0 3.358308803853358 0.0 48-49 0.0 0.0 0.0 3.5924538399785924 0.0 50-51 0.0 0.0 0.0 3.6861118544286864 0.0 52-53 0.0 0.0 0.0 3.7864597270537868 0.0 54-55 0.0 0.0 0.0 3.886807599678887 0.0 56-57 0.0 0.0 0.0 4.000535188654 0.0 58-59 0.0 0.0 0.0 4.114262777629115 0.0 60-61 0.0 0.0 0.0 4.174471501204175 0.0 62-63 0.0 0.0 0.0 4.248059941129248 0.0 64-65 0.0 0.0 0.0 4.3082686647043085 0.0 66-67 0.0 0.0 0.0 4.428686111854429 0.0 68-69 0.0 0.0 0.0 4.508964409954508 0.0 70-71 0.0 0.0 0.0 4.616002140754617 0.0 72-73 0.0 0.0 0.0 4.682900722504683 0.0 74-75 0.0 0.0 0.0 4.7431094460797425 0.0 76-77 0.0 0.0 0.0 4.91035590045491 0.0 78-79 0.0 0.0 0.0 5.017393631255017 0.0 80-81 0.0 0.0 0.0 5.077602354830077 0.0 82-83 0.0 0.0 0.0 5.137811078405138 0.0 84-85 0.0 0.0 0.0 5.211399518330211 0.0 86-87 0.0 0.0 0.0 5.345196681830345 0.0 88-89 0.0 0.0 0.0 5.405405405405405 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACAT 80 0.0 65.3125 5 TATACAC 90 0.0 58.055557 3 ATACACA 90 0.0 58.055557 4 CTTATAC 95 0.0 55.0 1 ACACATC 100 0.0 52.25 6 CACATCT 105 0.0 49.761906 7 TTATACA 105 0.0 49.761906 2 ACATCTC 110 0.0 47.5 8 CATCTCC 120 1.8189894E-12 43.541668 9 GTACATG 170 2.561137E-9 27.941177 1 TACATGG 170 2.561137E-9 27.941177 2 ACATGGG 160 4.6864443E-8 26.718752 3 GAGTACT 125 1.0913936E-11 24.699999 12-13 CATGGGA 120 1.4992642E-4 23.75 4 GTACTTT 135 3.092282E-11 22.870369 14-15 ATCTCCG 115 2.9576768E-9 22.717392 10-11 AGTACTT 125 7.748895E-9 20.9 12-13 ACTTTTT 150 1.2551027E-10 20.583334 16-17 GTCTTCT 130 1.2176315E-8 20.096155 48-49 GCTTGAA 130 1.2176315E-8 20.096155 56-57 >>END_MODULE