FastQCFastQC Report
Fri 10 Feb 2017
SRR2031411_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031411_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947791
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA352.800001E-454.237316
GGTATCA7900.049.8710981
GTATCAA15400.046.234741
TCAACGC18100.038.288844
CAACGCA18150.038.183365
ATCAACG18800.036.6107033
AACGCAG19200.036.334766
TATCAAC20350.034.2886852
ACGCAGA21250.033.052857
GTACATG27500.030.5519181
CGCAGAG22700.030.5234188
TACATGG29150.028.3340472
ACATGGG30600.026.3709283
CATGGGG19800.025.172254
GCAGAGT28500.024.1451179
GTTCGGA1252.2659164E-422.7844811
AGAGTAC23900.022.63670210-11
GGGTATA1805.1977186E-621.0967431
TAGCCCT7200.020.437474
CAGAGTA25500.020.19276210-11
Produced by FastQC (version 0.11.5)