FastQCFastQC Report
Fri 10 Feb 2017
SRR2031411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947791
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.12734875093770673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.050.9095761
GTATCAA17150.046.28571
TCAACGC20250.037.998254
CAACGCA20350.037.8115235
ATCAACG21600.036.0631523
AACGCAG21400.035.9562876
TATCAAC22450.035.1208762
ACGCAGA24350.031.4051237
CGCAGAG26200.029.1875868
GTACATG29100.028.9117761
TACATGG30700.026.9205822
ACATGGG31000.025.740753
GCAGAGT32300.024.1165359
CAGAGTA26650.022.54586410-11
AGAGTAC25600.021.98629210-11
GTCTATA1954.19177E-721.9382691
GTATAGT3101.0913936E-1121.466481
CATGGGG19700.021.2172974
GTATATA3650.020.8363481
GAGTACA21000.019.9185541
Produced by FastQC (version 0.11.5)