FastQCFastQC Report
Fri 10 Feb 2017
SRR2031408_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031408_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466930
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.18562576264716107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.128022468693121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.055.135361
GTATCAA30750.043.84931
ATCAACG36700.036.0995453
TCAACGC36800.036.0014464
CAACGCA37300.035.5188525
AACGCAG39200.034.022126
TATCAAC40900.032.6247062
ACGCAGA44750.029.9086827
CGCAGAG46050.028.7551578
GCAGAGT55150.023.9235529
GTACATG46700.023.8913351
TACATGG45300.023.7953012
ACATGGG45350.023.140813
AGAGTAC49600.021.14570210-11
CATGGGG28850.020.9036184
CAGAGTA52700.020.847410-11
GAGTACT31550.020.23179812-13
GTACTTT33600.018.6436714-15
GAGTACA34850.018.2554041
TAGCCCT12550.017.7835184
Produced by FastQC (version 0.11.5)