FastQCFastQC Report
Fri 10 Feb 2017
SRR2031408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466930
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34150.2327991110686945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.17308256017669554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.10211802880846392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.059.0617561
GTATCAA32750.044.423461
TCAACGC38000.038.2481584
ATCAACG38350.037.8990943
CAACGCA39350.036.9359635
AACGCAG41300.035.307026
TATCAAC41950.034.759952
ACGCAGA46300.031.4941657
CGCAGAG48350.030.0606028
GTACATG46600.026.4250661
GCAGAGT56350.025.5400319
TACATGG46050.025.4765172
ACATGGG45000.025.0154633
AGAGTAC50900.022.39577910-11
CAGAGTA53850.021.78632410-11
GAGTACT35650.021.51829712-13
CATGGGG28100.020.9598454
GTATAGA6700.020.5790541
GTACTTT39200.019.39012714-15
AGTACTT38950.018.96347212-13
Produced by FastQC (version 0.11.5)