##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1466930
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.27911147771196	34.0	33.0	34.0	31.0	34.0
2	33.38243406297506	34.0	34.0	34.0	31.0	34.0
3	33.47317049893314	34.0	34.0	34.0	31.0	34.0
4	36.65160914290389	37.0	37.0	37.0	35.0	37.0
5	36.5549712665226	37.0	37.0	37.0	35.0	37.0
6	36.600268588139855	37.0	37.0	37.0	36.0	37.0
7	36.61321058264539	37.0	37.0	37.0	36.0	37.0
8	36.618890471937995	37.0	37.0	37.0	36.0	37.0
9	38.5604350582509	39.0	39.0	39.0	38.0	39.0
10-11	38.563301589032875	39.0	39.0	39.0	38.0	39.0
12-13	38.567140558854206	39.0	39.0	39.0	38.0	39.0
14-15	40.27772661272181	41.0	40.5	41.0	39.0	41.0
16-17	40.32994212402773	41.0	41.0	41.0	39.0	41.0
18-19	40.350028631223026	41.0	41.0	41.0	39.0	41.0
20-21	40.3449830598597	41.0	41.0	41.0	39.0	41.0
22-23	40.30337234905551	41.0	41.0	41.0	39.0	41.0
24-25	40.276750765203516	41.0	41.0	41.0	39.0	41.0
26-27	40.20030096868972	41.0	40.0	41.0	39.0	41.0
28-29	40.128267197480454	41.0	40.0	41.0	38.0	41.0
30-31	40.00064352082239	41.0	40.0	41.0	38.0	41.0
32-33	39.88701676289939	41.0	40.0	41.0	38.0	41.0
34-35	39.814989808647994	41.0	40.0	41.0	38.0	41.0
36-37	39.73254347514878	41.0	40.0	41.0	37.5	41.0
38-39	39.634225559501814	41.0	40.0	41.0	37.0	41.0
40-41	39.535170730709716	41.0	40.0	41.0	37.0	41.0
42-43	39.35559297307984	41.0	40.0	41.0	36.5	41.0
44-45	39.23624474242125	41.0	40.0	41.0	36.0	41.0
46-47	39.095776212907225	41.0	39.5	41.0	36.0	41.0
48-49	38.900266202204605	41.0	39.0	41.0	35.0	41.0
50-51	38.806046300777815	41.0	39.0	41.0	35.0	41.0
52-53	38.822070923629624	41.0	39.0	41.0	35.0	41.0
54-55	38.76587737656193	41.0	39.0	41.0	35.0	41.0
56-57	38.696252036566165	41.0	39.0	41.0	35.0	41.0
58-59	38.66859257087932	41.0	39.0	41.0	35.0	41.0
60-61	38.49940965144894	41.0	38.0	41.0	35.0	41.0
62-63	38.30605550367093	40.0	37.0	41.0	35.0	41.0
64-65	38.05229526971294	40.0	37.0	41.0	35.0	41.0
66-67	37.77538089752068	39.0	36.0	41.0	35.0	41.0
68-69	37.44550489798422	39.0	36.0	41.0	34.5	41.0
70-71	37.1062129753976	38.5	35.0	41.0	34.0	41.0
72-73	36.761955239854665	37.0	35.0	40.0	34.0	41.0
74-75	36.37963774685909	37.0	35.0	39.0	34.0	41.0
76-77	35.16610710804197	35.5	34.5	39.0	32.0	39.5
78-79	35.55317670236481	36.0	35.0	38.5	33.5	39.5
80-81	35.31897432051973	35.5	35.0	37.0	34.0	39.0
82-83	35.006915803753415	35.0	35.0	37.0	33.5	39.0
84-85	34.71202681791224	35.0	35.0	36.5	33.0	38.0
86-87	34.48323266958887	35.0	35.0	36.0	33.0	37.0
88-89	34.28657331979031	35.0	35.0	36.0	33.0	37.0
90-91	34.122334399051084	35.0	35.0	35.5	33.0	36.5
92-93	34.01525703339628	35.0	35.0	35.0	33.0	36.0
94-95	33.91826365266236	35.0	35.0	35.0	33.0	36.0
96-97	33.838088047827775	35.0	35.0	35.0	33.0	36.0
98-99	33.777561983189386	35.0	35.0	35.0	33.0	36.0
100-101	33.51951660951784	35.0	34.5	35.0	31.5	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.04604521374313464
1101	2	0.02506112696927687
1101	3	0.1035546858837364
1101	4	0.10169159888248913
1101	5	0.023317771372425966
1101	6	0.019083712792003382
1101	7	-0.006593431927178983
1101	8	0.004428679182908013
1101	9	0.08132614634937596
1101	10-11	0.05648741837576665
1101	12-13	0.13485254974258254
1101	14-15	0.1659959448233863
1101	16-17	0.08152937395594506
1101	18-19	0.058509970635491015
1101	20-21	0.08073162750300611
1101	22-23	0.12035260134474157
1101	24-25	0.11049272124847676
1101	26-27	0.1020327432338135
1101	28-29	0.10567627592526208
1101	30-31	0.09743533056201414
1101	32-33	0.07790268671198675
1101	34-35	0.09811985460260786
1101	36-37	0.09527391165364918
1101	38-39	0.08379113390651582
1101	40-41	0.05263798001453779
1101	42-43	0.10895929394062165
1101	44-45	0.04670360027495235
1101	46-47	0.031886173865153467
1101	48-49	-0.04081668285736839
1101	50-51	0.006980158156778771
1101	52-53	0.02687929509686171
1101	54-55	0.03291126880045425
1101	56-57	0.04559648422229401
1101	58-59	0.03521239307368518
1101	60-61	0.06865629068729362
1101	62-63	0.05338746135393535
1101	64-65	0.15971218510364338
1101	66-67	0.09948614154514246
1101	68-69	0.0297893765384174
1101	70-71	0.05114335221581001
1101	72-73	-0.04477489444563787
1101	74-75	-0.018681031364202738
1101	76-77	-0.08753496745001854
1101	78-79	0.06883870562260341
1101	80-81	0.020483887313829996
1101	82-83	-0.037997783024920295
1101	84-85	-0.029490517389739068
1101	86-87	-0.11411036280636466
1101	88-89	-0.048345730831307776
1101	90-91	-0.0036896592173860654
1101	92-93	-0.060623363232004124
1101	94-95	-0.0020861652302670564
1101	96-97	-0.013028054909462128
1101	98-99	-0.018900263767974934
1101	100-101	0.07059531289102239
1102	1	0.15447265884067463
1102	2	0.09928881296777092
1102	3	0.10855951333763869
1102	4	0.12335129954889368
1102	5	0.12544531050310326
1102	6	0.11528496258365095
1102	7	0.11898452594974884
1102	8	0.0842052639872719
1102	9	0.10353495024152437
1102	10-11	0.07896794345180069
1102	12-13	0.10977148940097692
1102	14-15	0.1389018204794965
1102	16-17	0.10766681329827321
1102	18-19	0.06861428766868727
1102	20-21	0.08423915801660797
1102	22-23	0.046254171603848704
1102	24-25	0.047610967137273974
1102	26-27	0.08085044159184918
1102	28-29	0.022825216244100943
1102	30-31	0.023156382349434068
1102	32-33	0.09510226026068125
1102	34-35	0.1146398106995079
1102	36-37	0.09678001437305994
1102	38-39	0.054029801969527114
1102	40-41	0.14038819780032696
1102	42-43	0.18403673762293948
1102	44-45	0.0726784471959192
1102	46-47	0.055521199480537575
1102	48-49	0.044647806265928125
1102	50-51	0.19447909674590846
1102	52-53	0.2864595110271466
1102	54-55	0.2196481930047156
1102	56-57	0.2702966946175138
1102	58-59	0.2847829540961584
1102	60-61	0.2838902885424517
1102	62-63	0.32493630355123315
1102	64-65	0.30326525407490834
1102	66-67	0.2743723740240043
1102	68-69	0.35209032661908424
1102	70-71	0.3278237621877267
1102	72-73	0.24779491494069106
1102	74-75	0.2514214616147612
1102	76-77	0.09854278128964467
1102	78-79	0.2887674417102559
1102	80-81	0.23178641928981136
1102	82-83	0.2394569065241896
1102	84-85	0.17871835569068395
1102	86-87	0.2103954914642756
1102	88-89	0.17377470077767043
1102	90-91	0.15720670619728594
1102	92-93	0.26463066777062494
1102	94-95	0.11431742878558282
1102	96-97	0.09583118785643308
1102	98-99	0.1676684071569099
1102	100-101	0.2527466276252923
1103	1	0.010444825817472747
1103	2	0.036130993110603526
1103	3	0.03679012575069862
1103	4	0.05490217016078702
1103	5	0.0640937443027525
1103	6	0.03130276310377411
1103	7	0.025474886767739235
1103	8	0.06817009450353595
1103	9	0.0010699208417435102
1103	10-11	0.016219153795873353
1103	12-13	0.028111277309925242
1103	14-15	0.08940768413490474
1103	16-17	0.07142080949370211
1103	18-19	0.046933612211631726
1103	20-21	0.08353283992271798
1103	22-23	0.035535209141556834
1103	24-25	0.061741774995631715
1103	26-27	0.01924071212679479
1103	28-29	0.03449769331321306
1103	30-31	0.13720348721587783
1103	32-33	0.14549478105453062
1103	34-35	0.08121255849918896
1103	36-37	0.0675527317029676
1103	38-39	-0.07693929728075943
1103	40-41	0.020851789073638827
1103	42-43	0.15292258085056432
1103	44-45	0.13363847071811819
1103	46-47	0.08506147917420748
1103	48-49	0.2391440381492984
1103	50-51	0.2313638397415616
1103	52-53	0.2630351220382394
1103	54-55	0.31615294101543867
1103	56-57	0.2464766133687064
1103	58-59	0.3329821245354836
1103	60-61	0.35965009178114116
1103	62-63	0.3681669426401726
1103	64-65	0.3653355871144086
1103	66-67	0.3342292015976156
1103	68-69	0.32227498893501405
1103	70-71	0.2878576583426664
1103	72-73	0.24904808862974193
1103	74-75	0.33105371382396953
1103	76-77	0.16883373217816455
1103	78-79	0.26387380438251995
1103	80-81	0.23786970613794978
1103	82-83	0.36391205162025386
1103	84-85	0.2354386922573326
1103	86-87	0.22370053255402667
1103	88-89	0.24866487024065975
1103	90-91	0.1949598973738489
1103	92-93	0.13370276793915536
1103	94-95	0.1404479378740504
1103	96-97	0.10059342571401686
1103	98-99	0.1981432319408185
1103	100-101	0.24390505450700317
1104	1	0.007596869556373065
1104	2	0.028999117517628292
1104	3	-0.02951781028651368
1104	4	0.019252268660274297
1104	5	-0.004385974391190928
1104	6	0.01602168559632844
1104	7	-0.041455708722132556
1104	8	-0.03612174046327965
1104	9	0.010720306199374363
1104	10-11	0.05453054818514147
1104	12-13	0.08032102156802523
1104	14-15	0.08311419691865041
1104	16-17	0.026960583329518784
1104	18-19	0.020944687411279972
1104	20-21	0.06231392133222613
1104	22-23	0.012565931839027655
1104	24-25	0.01866650622638133
1104	26-27	-0.014674438955914582
1104	28-29	-0.00444639443216488
1104	30-31	0.041953314131049524
1104	32-33	0.08372272681348392
1104	34-35	0.026077931386915054
1104	36-37	-0.017280577288865118
1104	38-39	0.0385018767305354
1104	40-41	-0.046693661721768365
1104	42-43	0.10828620393700561
1104	44-45	0.16750846923532947
1104	46-47	0.00924391114712364
1104	48-49	0.06309557885516881
1104	50-51	0.1985827984676547
1104	52-53	0.112717493250301
1104	54-55	0.12868227624703366
1104	56-57	0.22309706090309334
1104	58-59	0.2561204731110607
1104	60-61	0.14554972598961768
1104	62-63	0.15230980758717294
1104	64-65	0.1449121898353809
1104	66-67	0.10834011413653855
1104	68-69	0.06406196024585853
1104	70-71	0.14776429844550165
1104	72-73	0.21492724252640727
1104	74-75	0.17911811810554212
1104	76-77	0.055936710129223854
1104	78-79	0.23808890228554702
1104	80-81	0.26520651863440037
1104	82-83	0.24393081501968794
1104	84-85	0.2403168416487773
1104	86-87	0.1128782987618493
1104	88-89	0.21952752951493437
1104	90-91	0.21961682149189699
1104	92-93	0.22491791824145935
1104	94-95	0.12445919145420703
1104	96-97	0.17288407668022643
1104	98-99	0.1645277043637492
1104	100-101	0.1707567835157917
1105	1	0.04369185124856756
1105	2	0.02763788836730896
1105	3	0.023090152591329627
1105	4	-0.009977156819729771
1105	5	0.03990556628881592
1105	6	-0.039509897317223874
1105	7	0.01103291209008006
1105	8	0.027317687445254535
1105	9	0.037195483570869214
1105	10-11	0.0536318346821858
1105	12-13	0.09750315215222116
1105	14-15	0.09618293482159146
1105	16-17	0.08647058252695672
1105	18-19	0.06673139135019568
1105	20-21	0.07393075096074853
1105	22-23	0.10184630439473352
1105	24-25	0.08487347142539647
1105	26-27	0.0101987405564401
1105	28-29	0.11030881883576171
1105	30-31	0.058787320261792786
1105	32-33	0.11226945314076175
1105	34-35	0.11484237099514161
1105	36-37	0.08640787425564866
1105	38-39	0.1004914809422246
1105	40-41	0.13264463082158784
1105	42-43	0.21977879274261625
1105	44-45	0.2572604774652447
1105	46-47	0.1888784742769971
1105	48-49	0.2583519662614009
1105	50-51	0.35131119781435416
1105	52-53	0.30353581501944404
1105	54-55	0.24154964395713563
1105	56-57	0.218063848371024
1105	58-59	0.30719288403967226
1105	60-61	0.3639376283521756
1105	62-63	0.33791069228189485
1105	64-65	0.2721790376518385
1105	66-67	0.324968111889369
1105	68-69	0.29945402238293894
1105	70-71	0.34435611835467483
1105	72-73	0.34859817319648556
1105	74-75	0.3201085217527222
1105	76-77	0.19547051520245873
1105	78-79	0.2547603113661765
1105	80-81	0.3332935122253602
1105	82-83	0.3807924522260677
1105	84-85	0.2998685323975181
1105	86-87	0.23687574723890492
1105	88-89	0.20839588531480757
1105	90-91	0.2541408134162424
1105	92-93	0.3078015039650239
1105	94-95	0.31029797856359664
1105	96-97	0.20349323193643443
1105	98-99	0.24334532154567512
1105	100-101	0.34425982230873586
1106	1	0.05428068728286206
1106	2	0.07634474244382972
1106	3	0.02484152098787007
1106	4	0.056238902019082104
1106	5	0.12613918360362675
1106	6	-0.043124614186794474
1106	7	0.02743127449343774
1106	8	0.09984494339587258
1106	9	0.09246437013057118
1106	10-11	0.011661702008190389
1106	12-13	0.04616700587936151
1106	14-15	0.04873025760192462
1106	16-17	-0.019982157401550182
1106	18-19	-0.05766951524050512
1106	20-21	-0.0304167635294732
1106	22-23	-0.006666868941465509
1106	24-25	0.019916068646502083
1106	26-27	0.008089035915084253
1106	28-29	0.03946798283729436
1106	30-31	0.11392407033121543
1106	32-33	-0.12017655006635408
1106	34-35	0.07340750308683397
1106	36-37	0.06890090909489288
1106	38-39	-0.029439001826290223
1106	40-41	0.0960428230152317
1106	42-43	0.14435573201358665
1106	44-45	0.16899106235958072
1106	46-47	-0.17091575113442303
1106	48-49	0.019935185375061337
1106	50-51	0.11954603347238901
1106	52-53	-0.058538324749612514
1106	54-55	0.25406677690884294
1106	56-57	0.347235106220829
1106	58-59	0.306939809066769
1106	60-61	0.2558074268776167
1106	62-63	0.1749572849521286
1106	64-65	0.19700905189569795
1106	66-67	0.19033222630613267
1106	68-69	-0.03371358043715844
1106	70-71	0.15863492186995387
1106	72-73	0.20480231735515275
1106	74-75	0.1637753765459209
1106	76-77	-0.049154441125317305
1106	78-79	0.1507935291360667
1106	80-81	0.2321575863293006
1106	82-83	0.17190257791250474
1106	84-85	0.08419714465083672
1106	86-87	0.06067061765556048
1106	88-89	-0.16222233429155608
1106	90-91	0.1043831383017988
1106	92-93	0.11621479107297716
1106	94-95	0.160461917395601
1106	96-97	0.13253817625993491
1106	98-99	0.1970572371216548
1106	100-101	0.21246151186412732
1107	1	-0.021564066352453892
1107	2	-0.015200746972773516
1107	3	-0.032379132032552604
1107	4	-0.01845510363004621
1107	5	-0.024347268954173273
1107	6	-0.003197146181079802
1107	7	-0.018319675745424036
1107	8	0.009810537193459368
1107	9	-0.017510522640634463
1107	10-11	0.011882552631803378
1107	12-13	0.03173180228996131
1107	14-15	0.03483991574839962
1107	16-17	-0.043209249961215335
1107	18-19	-0.009295963846241762
1107	20-21	-0.011346602773464554
1107	22-23	-0.0520734665632574
1107	24-25	-0.023322705748768158
1107	26-27	-0.03330447340132281
1107	28-29	-0.03720350767193281
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2316	20-21	-0.1345347500923637
2316	22-23	-0.15102614071513187
2316	24-25	-0.15227841642305862
2316	26-27	-0.1622226848530417
2316	28-29	-0.16532326006731068
2316	30-31	-0.4744047522126422
2316	32-33	-0.6237165391956268
2316	34-35	-0.5312470152673114
2316	36-37	-0.2830569416400053
2316	38-39	-0.19634241489693238
2316	40-41	-0.22901559310929542
2316	42-43	-0.2152144261722313
2316	44-45	-0.19616855234615116
2316	46-47	-0.1384674851267178
2316	48-49	-0.17975970594573454
2316	50-51	-0.21645595015428398
2316	52-53	-0.20740978686178835
2316	54-55	-0.16232812169645428
2316	56-57	-0.10351043482175015
2316	58-59	-0.14447954866405155
2316	60-61	-0.392170282537883
2316	62-63	-0.388807347096332
2316	64-65	-0.1633329836190569
2316	66-67	-0.1533451037547806
2316	68-69	-0.34257658131691215
2316	70-71	-0.2698452502684958
2316	72-73	-0.20337369869317712
2316	74-75	-0.2877882704133654
2316	76-77	-0.25872543983091845
2316	78-79	-0.2939478989630757
2316	80-81	-0.20085298740949753
2316	82-83	-0.157008220525249
2316	84-85	-0.11197877053511718
2316	86-87	-0.22615535552625232
2316	88-89	-0.12595390589544309
2316	90-91	-0.20549128804344718
2316	92-93	-0.1528061714902691
2316	94-95	-0.13405201001711475
2316	96-97	-0.19664859283806635
2316	98-99	-0.4535274844701789
2316	100-101	-0.5535784072218277
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	10.0
14	23.0
15	63.0
16	202.0
17	542.0
18	1024.0
19	1832.0
20	2743.0
21	4202.0
22	6101.0
23	6763.0
24	6094.0
25	4901.0
26	4130.0
27	4294.0
28	4762.0
29	5835.0
30	6941.0
31	8668.0
32	11300.0
33	15260.0
34	23143.0
35	40562.0
36	90267.0
37	275771.0
38	625581.0
39	315276.0
40	637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.285739239965636	19.905304464773714	14.17696833724657	23.631987958014083
2	14.361198103779488	21.29996359714722	41.3687063625915	22.970131936481796
3	17.056505763737874	27.444731514114512	31.345803821586575	24.152958900561035
4	11.525976017942233	18.464480241047628	41.337691641727965	28.671852099282173
5	12.4714539111039	38.71367744521537	36.1575839544735	12.657284689207227
6	28.084162161793678	39.73775163095717	19.630520883750417	12.547565323498736
7	25.32097646104449	33.58824211107551	24.129099548035693	16.9616818798443
8	24.650051468031876	35.87424076131786	21.77336341884071	17.70234435180956
9	26.15475857743723	16.032462353350194	21.1012113734125	36.71156769580007
10-11	23.24650801333397	26.85322408022196	30.527564369124633	19.372703537319435
12-13	24.7642355122603	24.501578125745603	29.682329763519732	21.051856598474362
14-15	21.796847838683508	25.80392384094674	27.62006367038645	24.779164649983297
16-17	21.39328393311201	28.92039838301759	27.826753832834562	21.859563851035837
18-19	21.206783127396676	28.13654878568385	29.729799744354494	20.926868342564976
20-21	22.42006674781481	27.301226527739953	29.47133153743921	20.807375187006024
22-23	21.883286864403892	27.26019646472565	29.146550960168515	21.709965710701944
24-25	21.661531225075496	27.363916478632245	29.24464698383699	21.729905312455262
26-27	21.741732795553602	27.616691168525637	29.061272599172877	21.58030343674788
28-29	21.68218660740458	27.63284546638217	29.176102472510618	21.50886545370263
30-31	22.005480834122963	27.34496533576926	29.10486526282781	21.544688567279966
32-33	21.755332565289415	27.52411498844526	29.11188672942813	21.608665716837205
34-35	21.988711117776578	27.49834688771789	29.036286666712112	21.47665532779342
36-37	22.05428263002877	27.36440505516238	28.906265711316376	21.675046603492476
38-39	21.886024441837304	27.576212871810217	28.96689028850421	21.570872397848266
40-41	22.136502764276415	27.483520004362855	28.85519418104477	21.524783050315964
42-43	22.025488605454928	27.44285685070181	28.802703605489015	21.72895093835425
44-45	22.192844921025543	27.594534163184335	28.5474767030465	21.66514421274362
46-47	22.0559102365281	27.56924006301416	28.348360162533165	22.026489537924576
48-49	22.182781863267238	27.508968667183627	28.484895997545866	21.823353472003273
50-51	22.19475366922757	27.753198857477862	28.172407681348123	21.879639791946445
52-53	22.18084707518423	27.795634420183653	28.130108457799626	21.8934100468325
54-55	22.265343267913263	27.672383821995595	28.173532479395746	21.888740430695396
56-57	22.26421846986564	27.818709822554588	28.03964742693925	21.87742428064052
58-59	22.222532772524932	27.702617030124138	28.19135882421111	21.883491373139822
60-61	22.352050937698124	27.813946512690112	28.08090586206191	21.75309668754985
62-63	22.130447632068996	27.837765885827046	28.20321668972678	21.828569792377177
64-65	22.225009791526553	27.693982156753734	28.13329701918321	21.947711032536503
66-67	22.25441436675571	27.83886142520229	27.936653585672783	21.970070622369214
68-69	22.263264977092824	27.655458097475726	28.091002888066214	21.99027403736523
70-71	22.26772948236476	27.52779461197979	28.090810616249957	22.113665289405496
72-73	22.19408258691341	27.725611514420635	27.974709777345417	22.105596121320534
74-75	22.175861999618924	27.815804067709028	27.80721449293047	22.201119439741575
76-77	22.13508806015427	27.67883863534881	27.949171549429234	22.236901755067677
78-79	22.177983952881185	27.705548322005825	27.834525164799956	22.281942560313034
80-81	22.26745652485122	27.765503466423073	27.700571942764824	22.266468065960883
82-83	22.313743668750384	27.83159387291827	27.79457779171467	22.060084666616675
84-85	22.468761290586464	27.59030764930842	27.82368620179559	22.117244858309533
86-87	22.17690821852113	27.736396794790735	27.87583819071806	22.21085679597007
88-89	22.344512497524562	27.632667432675685	27.826169353890368	22.19665071590939
90-91	22.30557117780391	27.845629462992317	27.726366378649214	22.122432980554564
92-93	22.379902244824223	27.749381361073805	27.787147307642492	22.083569086459477
94-95	22.294574901214332	27.706515899788116	27.839457271961233	22.159451927036322
96-97	22.2806473383188	27.765844314316297	27.85944114579428	22.094067201570628
98-99	22.267081592168676	27.680325577907606	27.789362818948415	22.263230010975303
100-101	22.21203465741378	27.78281853939861	27.842023818450777	22.16312298473683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	10.5
2	24.0
3	50.5
4	44.5
5	40.5
6	72.0
7	150.0
8	325.0
9	477.0
10	483.0
11	488.0
12	541.5
13	610.5
14	708.5
15	809.0
16	901.5
17	996.0
18	1121.0
19	1256.5
20	1469.0
21	1763.5
22	2193.0
23	2872.0
24	3888.0
25	5275.0
26	7223.0
27	9960.5
28	13094.0
29	17072.0
30	21654.5
31	26253.0
32	31408.5
33	36158.0
34	40621.5
35	44305.5
36	47760.0
37	50028.5
38	50013.5
39	50216.0
40	50327.0
41	50255.5
42	50197.0
43	50945.5
44	52399.0
45	53771.0
46	53997.5
47	53628.0
48	53389.0
49	52560.0
50	50944.0
51	48592.0
52	46129.5
53	44149.0
54	42108.5
55	38921.5
56	35149.0
57	31189.0
58	27922.5
59	24353.5
60	19841.0
61	15883.5
62	12420.0
63	9265.5
64	6787.5
65	5038.5
66	3794.5
67	2823.5
68	2047.5
69	1477.5
70	1147.5
71	844.0
72	617.5
73	497.5
74	360.5
75	259.5
76	193.5
77	136.5
78	84.0
79	46.0
80	26.5
81	21.0
82	19.5
83	9.0
84	6.5
85	3.0
86	1.0
87	1.0
88	0.5
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09482388389357366
2	8.180349437260128E-4
3	0.0
4	0.0
5	2.726783145753376E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0037493268254108923
20-21	0.004465107401171153
22-23	0.0
24-25	0.0
26-27	0.002351850463212287
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0022836808845684525
38-39	0.0012952219942328536
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.018780718916376376
48-49	0.001192967626267102
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0027267831457533764
62-63	0.007600908018787535
64-65	0.006851042653705357
66-67	0.0029653766710067966
68-69	0.002147341727280784
70-71	6.135262077945097E-4
72-73	0.002658613567109542
74-75	0.002420020041856121
76-77	0.002385935252534204
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.749326825410892E-4
88-89	0.001329306783554771
90-91	3.4084789321917205E-4
92-93	0.0
94-95	0.00804401027997246
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1466930.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.90377731778595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.57916055614736	41.573791566755666
2	16.329951219890916	19.23791620533523
3	6.002004938931706	10.606222871492541
4	2.85064739838704	6.7165559826446435
5	1.4332206040448936	4.221105365396154
6	0.8374376050632855	2.9596942923712652
7	0.5254971414607221	2.1667636621214807
8	0.35506667993960195	1.6731814918502312
9	0.2400956644519641	1.2728287398459917
>10	0.8186823607681298	7.475758808621648
>50	0.020344011066526464	0.8135523257426428
>100	0.007427618146611807	0.7246900054615566
>500	1.1605042529525393E-4	0.048534239705504544
>1k	3.4815127588576177E-4	0.509404442655521
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3415	0.2327991110686945	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2539	0.17308256017669554	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1498	0.10211802880846392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	1.7042394660958602E-4	0.0
20-21	0.0	0.0	0.0	2.385935252534204E-4	0.0
22-23	0.0	0.0	0.0	3.067631038972548E-4	0.0
24-25	0.0	0.0	0.0	3.40847893219172E-4	0.0
26-27	0.0	0.0	0.0	4.4310226118492364E-4	0.0
28-29	0.0	0.0	0.0	4.771870505068408E-4	0.0
30-31	0.0	0.0	0.0	7.498653650821784E-4	0.0
32-33	0.0	0.0	0.0	0.0016360698874520257	0.0
34-35	0.0	0.0	0.0	0.0027608679350752934	0.0
36-37	0.0	0.0	0.0	0.004771870505068408	0.0
38-39	0.0	0.0	0.0	0.007873586333362874	0.0
40-41	0.0	0.0	0.0	0.011622913158773767	0.0
42-43	0.0	0.0	0.0	0.014383781093849059	0.0
44-45	0.0	0.0	0.0	0.01809902312993803	0.0
46-47	0.0	0.0	0.0	0.024063861261273543	0.0
48-49	0.0	0.0	0.0	0.02897207092362962	0.0
50-51	0.0	0.0	0.0	0.03449380679378021	0.0
52-53	0.0	0.0	0.0	0.03974286434935545	0.0
54-55	0.0	0.0	0.0	0.04444656527578003	0.0
56-57	0.0	0.0	0.0	0.05068408172169088	0.0
58-59	0.0	0.0	0.0	0.05855766805505375	0.0
60-61	0.0	0.0	0.0	0.06666984791367005	0.0
62-63	0.0	0.0	0.0	0.07444117987906718	0.0
64-65	0.0	0.0	0.0	0.08091728985023144	0.0
66-67	0.0	0.0	0.0	0.08885904576223815	0.0
68-69	0.0	0.0	0.0	0.09857321071898455	0.0
70-71	0.0	0.0	0.0	0.11053697177097749	0.0
72-73	0.0	0.0	0.0	0.12287566550551152	0.0
74-75	0.0	0.0	0.0	0.13456674824292914	0.0
76-77	0.0	0.0	0.0	0.14656459408424397	0.0
78-79	0.0	0.0	0.0	0.16180049491114096	0.0
80-81	0.0	0.0	0.0	0.1775135827885448	0.0
82-83	0.0	0.0	0.0	0.19278356840476368	0.0
84-85	0.0	0.0	0.0	0.2102690653269072	0.0
86-87	0.0	0.0	0.0	0.22635708588685213	0.0
88-89	0.0	0.0	0.0	0.2429563782866258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1610	0.0	59.061756	1
GTATCAA	3275	0.0	44.42346	1
TCAACGC	3800	0.0	38.248158	4
ATCAACG	3835	0.0	37.899094	3
CAACGCA	3935	0.0	36.935963	5
AACGCAG	4130	0.0	35.30702	6
TATCAAC	4195	0.0	34.75995	2
ACGCAGA	4630	0.0	31.494165	7
CGCAGAG	4835	0.0	30.060602	8
GTACATG	4660	0.0	26.425066	1
GCAGAGT	5635	0.0	25.540031	9
TACATGG	4605	0.0	25.476517	2
ACATGGG	4500	0.0	25.015463	3
AGAGTAC	5090	0.0	22.395779	10-11
CAGAGTA	5385	0.0	21.786324	10-11
GAGTACT	3565	0.0	21.518297	12-13
CATGGGG	2810	0.0	20.959845	4
GTATAGA	670	0.0	20.579054	1
GTACTTT	3920	0.0	19.390127	14-15
AGTACTT	3895	0.0	18.963472	12-13
>>END_MODULE