FastQCFastQC Report
Fri 10 Feb 2017
SRR2031406_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031406_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090079
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.275943303191787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.22695602795760675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13880.1273302210206783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.060.2959061
GTATCAA28950.045.9248621
TCAACGC36200.036.3387074
ATCAACG37150.035.409453
AACGCAG37550.035.2641336
CAACGCA37600.035.111975
TATCAAC38900.034.303232
ACGCAGA43750.029.8327677
CGCAGAG45300.029.0215448
GTACATG36600.027.8930361
TACATGG37050.026.6595862
GCAGAGT51250.025.3732249
ACATGGG39000.024.718933
GAGTACT29400.023.32488812-13
AGAGTAC45050.022.85935610-11
CAGAGTA48800.022.31834210-11
CATGGGG26700.021.3436284
ACCGTAT1656.362171E-520.1350658
AGTACTT32900.020.12228812-13
GTACTTT33550.020.0148814-15
Produced by FastQC (version 0.11.5)