FastQCFastQC Report
Fri 10 Feb 2017
SRR2031406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090079
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.32603141607167924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.26089852203372416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.15329164216538435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.063.2903021
GTATCAA31850.048.0617331
TCAACGC40400.037.6203654
ATCAACG41350.036.756053
CAACGCA41450.036.5527885
AACGCAG42500.035.873236
TATCAAC42900.035.538752
ACGCAGA48800.030.9500757
CGCAGAG51200.029.4065258
GTACATG38400.028.4740751
GCAGAGT55850.026.8731359
TACATGG40000.026.241382
ACATGGG41650.024.4035593
GAGTACT31550.023.93658312-13
AGAGTAC49150.023.86864910-11
GTATAGG3350.022.705351
CAGAGTA53400.022.45817810-11
CATGGGG28450.021.2019524
GTACTTT35850.021.00265514-15
AGTACTT34950.020.99643912-13
Produced by FastQC (version 0.11.5)