FastQCFastQC Report
Fri 10 Feb 2017
SRR2031405_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031405_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377263
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23350.16953915120060584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.11864110195365736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.051.6636161
GTATCAA26350.046.1281781
TCAACGC29500.041.047654
ATCAACG30300.040.12063
CAACGCA30100.039.7561345
AACGCAG31500.037.8233156
TATCAAC33200.036.6147582
ACGCAGA37400.031.7296167
CGCAGAG38550.030.783088
TACATGG37550.025.9237752
GCAGAGT45700.025.6553069
GTACATG39700.025.2347451
ACATGGG39050.024.3208663
AGAGTAC41100.021.13363310-11
CAGAGTA44800.020.1828210-11
GAGTACT29150.019.8648912-13
CATGGGG27750.019.5079634
GTATAAT8550.019.436091
ATTATAC7100.019.3958913
GTATTAA8600.019.3230881
Produced by FastQC (version 0.11.5)