FastQCFastQC Report
Fri 10 Feb 2017
SRR2031405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377263
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28760.20881995668220232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.139552140731291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG200.002074931771.243363
GGTATCA15200.052.8670731
GTATCAA29500.045.6148871
TCAACGC34200.039.024144
ATCAACG34250.038.9671673
CAACGCA35100.038.023525
TATCAAC36900.036.297432
AACGCAG36950.036.248316
ACGCAGA40600.032.872557
CGCAGAG41650.031.7017278
GCAGAGT49450.026.5091559
GTACATG37950.025.5599821
TACATGG36450.024.887942
ACATGGG35300.024.7568993
AGAGTAC44300.022.56843810-11
CAGAGTA47850.021.48963510-11
GTATAAT8800.021.072891
GTATAGC7900.021.066051
CATGGGG25750.020.8427164
GTATATA9600.020.8027231
Produced by FastQC (version 0.11.5)