FastQCFastQC Report
Fri 10 Feb 2017
SRR2031404_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031404_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908614
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.19414184681283803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13340.14681702020880152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.061.6262361
GTATCAA18500.042.0570641
TCAACGC21800.034.831644
ATCAACG22300.034.2634773
CAACGCA22250.034.127185
AACGCAG23150.032.9945146
TATCAAC23900.031.9661662
GTACATG28450.030.3498081
TACATGG27900.030.1045132
ACGCAGA25800.029.6055437
ACACCGC1004.9930866E-528.4654526
CGCAGAG27550.027.5527678
ACATGGG29650.027.5305063
CGCGAAT350.00834775427.17283678-79
CATGGGG20350.025.1866154
TAACGCA1000.001661890123.7290554
GCAGAGT32850.022.5297349
CAGAGTA29700.021.5623610-11
ATGGGGG9150.020.7467145
GTATAGG2801.5988917E-920.3324641
Produced by FastQC (version 0.11.5)