##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031404_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	908614
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90057934392382	34.0	33.0	34.0	31.0	34.0
2	32.98829425916836	34.0	33.0	34.0	31.0	34.0
3	33.163157292315546	34.0	34.0	34.0	31.0	34.0
4	36.113579583849685	37.0	37.0	37.0	35.0	37.0
5	36.08441098200116	37.0	37.0	37.0	35.0	37.0
6	36.15899931103857	37.0	37.0	37.0	35.0	37.0
7	36.20066716999738	37.0	37.0	37.0	35.0	37.0
8	36.29118855751727	37.0	37.0	37.0	35.0	37.0
9	38.24820770976454	39.0	39.0	39.0	37.0	39.0
10-11	38.33947033613833	39.0	39.0	39.0	37.5	39.0
12-13	38.394512411210926	39.0	39.0	39.0	38.0	39.0
14-15	40.04472691373895	41.0	40.0	41.0	38.0	41.0
16-17	40.04350747402087	41.0	40.0	41.0	38.0	41.0
18-19	40.05368836491624	41.0	40.0	41.0	38.5	41.0
20-21	39.98737417649299	41.0	40.0	41.0	38.5	41.0
22-23	39.922298137602986	41.0	40.0	41.0	38.0	41.0
24-25	39.816984440037245	41.0	40.0	41.0	38.0	41.0
26-27	39.73135016629724	41.0	40.0	41.0	38.0	41.0
28-29	39.658707107748725	41.0	40.0	41.0	38.0	41.0
30-31	39.514818173613875	41.0	40.0	41.0	37.5	41.0
32-33	39.354673161540546	41.0	40.0	41.0	37.0	41.0
34-35	39.27246278397648	41.0	40.0	41.0	36.0	41.0
36-37	39.15894758390252	41.0	40.0	41.0	36.0	41.0
38-39	38.9860749449161	41.0	39.0	41.0	35.0	41.0
40-41	38.87147017325289	41.0	39.0	41.0	35.0	41.0
42-43	39.07343327309506	41.0	39.5	41.0	35.5	41.0
44-45	39.06415760708067	41.0	40.0	41.0	35.5	41.0
46-47	38.91467718965369	41.0	39.5	41.0	35.0	41.0
48-49	38.73476635843163	41.0	39.0	41.0	35.0	41.0
50-51	37.70970676216743	40.0	38.0	40.5	34.0	40.5
52-53	37.76399549203512	39.5	38.0	40.5	34.0	41.0
54-55	38.17095378235422	40.0	38.0	41.0	35.0	41.0
56-57	38.15819699014103	40.5	38.0	41.0	34.5	41.0
58-59	38.15790368627382	41.0	37.5	41.0	35.0	41.0
60-61	37.9391287169249	40.0	37.0	41.0	34.5	41.0
62-63	37.67138245723707	40.0	36.5	41.0	34.0	41.0
64-65	37.38395457256877	39.0	36.0	41.0	34.0	41.0
66-67	37.11161120123617	39.0	35.0	41.0	34.0	41.0
68-69	36.78060925761655	38.5	35.0	41.0	34.0	41.0
70-71	36.40542463576392	37.0	35.0	40.5	33.0	41.0
72-73	36.042108640192644	37.0	35.0	39.0	33.0	41.0
74-75	35.66344014069781	36.0	35.0	39.0	33.0	41.0
76-77	35.30627582229638	36.0	35.0	39.0	33.0	40.5
78-79	34.935171040727965	35.0	35.0	37.0	32.0	39.0
80-81	34.57060919158191	35.0	35.0	37.0	32.0	39.0
82-83	34.27630820128239	35.0	35.0	36.5	32.0	39.0
84-85	34.00209770045366	35.0	35.0	36.0	31.0	37.0
86-87	33.78378002099902	35.0	35.0	36.0	31.0	37.0
88-89	33.614921737943725	35.0	35.0	35.5	31.0	36.5
90-91	33.42288144360532	35.0	35.0	35.0	31.0	36.0
92-93	33.361596343441775	35.0	35.0	35.0	31.0	36.0
94-95	33.25893008472245	35.0	34.5	35.0	30.0	36.0
96-97	33.1856949155527	35.0	34.0	35.0	30.0	36.0
98-99	33.118058933716625	35.0	34.0	35.0	30.0	35.5
100-101	32.778593550176424	35.0	33.5	35.0	28.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10540580643648667
1101	2	0.1443931336692046
1101	3	0.08510695903536458
1101	4	0.10068550680332322
1101	5	-0.006099051342594919
1101	6	0.08260099777297114
1101	7	0.13111141081255084
1101	8	0.09945973783895568
1101	9	0.1797555976907219
1101	10-11	0.1289612756583267
1101	12-13	0.09225463672121492
1101	14-15	0.02177739373190235
1101	16-17	0.045864452273782774
1101	18-19	-0.012295889774726732
1101	20-21	0.08869168791603244
1101	22-23	0.08884288031906351
1101	24-25	0.07249918848889791
1101	26-27	-0.025165711383706935
1101	28-29	0.016892550223275293
1101	30-31	0.10636124823297877
1101	32-33	0.03948076314790683
1101	34-35	-0.03486942448496677
1101	36-37	0.09459736707162136
1101	38-39	-0.008675969996907895
1101	40-41	0.14830001557799477
1101	42-43	0.12727140216001231
1101	44-45	0.129235294087799
1101	46-47	0.07337031073726763
1101	48-49	0.20731366794900197
1101	50-51	0.036771505234291624
1101	52-53	-0.02188583927721055
1101	54-55	0.16309369989444633
1101	56-57	0.23712988607446306
1101	58-59	0.04448316035205835
1101	60-61	0.020197156155994378
1101	62-63	-0.09616052046785484
1101	64-65	-0.019092844697901512
1101	66-67	-0.07083755656596225
1101	68-69	-0.0820380405995067
1101	70-71	0.05847170185736417
1101	72-73	-0.10384605179373096
1101	74-75	-0.17825216125249455
1101	76-77	-0.19427307644994585
1101	78-79	-0.16484420191545013
1101	80-81	-0.24677330414906606
1101	82-83	-0.18402864446967726
1101	84-85	-0.12320995665559309
1101	86-87	-0.05886513520576386
1101	88-89	0.011032648878554596
1101	90-91	-0.08292927712601994
1101	92-93	-0.28684740320990443
1101	94-95	-0.13772188678171204
1101	96-97	-0.11728192211953115
1101	98-99	-0.32001626359128466
1101	100-101	-0.29745709278409294
1102	1	0.1478890048245134
1102	2	0.06359181657026625
1102	3	0.08660282467161551
1102	4	0.047196791834835494
1102	5	0.10417419754241308
1102	6	0.11027604783993183
1102	7	0.09122370824101722
1102	8	0.008915328751953666
1102	9	0.00513555370551444
1102	10-11	0.11642917751478876
1102	12-13	0.14660493343706804
1102	14-15	0.10870500243556336
1102	16-17	0.13194737298983483
1102	18-19	0.11437659244074183
1102	20-21	0.18697958215495447
1102	22-23	0.23737895924670482
1102	24-25	0.2640774956727441
1102	26-27	0.27529134160741364
1102	28-29	0.2169330952466808
1102	30-31	0.22981777291015248
1102	32-33	0.36324045402282223
1102	34-35	0.2334679838279925
1102	36-37	0.3031539028065282
1102	38-39	0.2867590289587483
1102	40-41	0.22499922064981348
1102	42-43	0.3441089517662377
1102	44-45	0.42660232484058014
1102	46-47	0.42110531829339237
1102	48-49	0.3949803018520086
1102	50-51	0.3246580651733453
1102	52-53	0.4343639303533422
1102	54-55	0.6339074262278217
1102	56-57	0.507953748663688
1102	58-59	0.5026618901870492
1102	60-61	0.571251633923815
1102	62-63	0.5516383762160544
1102	64-65	0.48561283529128474
1102	66-67	0.5820002879131536
1102	68-69	0.5610751414476525
1102	70-71	0.5438524564862561
1102	72-73	0.45586417415263014
1102	74-75	0.4252036876742906
1102	76-77	0.4322396829460544
1102	78-79	0.48673428956394815
1102	80-81	0.5548908847661735
1102	82-83	0.44705003744846294
1102	84-85	0.5426115100913407
1102	86-87	0.5430103792860095
1102	88-89	0.5788856670255242
1102	90-91	0.6161160718683405
1102	92-93	0.4982117556651602
1102	94-95	0.3789553256864977
1102	96-97	0.526494723946989
1102	98-99	0.4533585567205378
1102	100-101	0.4359040896562263
1103	1	-0.02675380679555417
1103	2	0.013168289067046146
1103	3	0.05595646723989489
1103	4	-0.1616034260124124
1103	5	0.048498581370736815
1103	6	-0.04144934874635453
1103	7	-0.0580393191405264
1103	8	-0.07800340727047939
1103	9	-0.022219919706692792
1103	10-11	0.11657112585717755
1103	12-13	0.16086784494242323
1103	14-15	0.11226240171017565
1103	16-17	0.14226469995769975
1103	18-19	0.08602748063180599
1103	20-21	0.15849866117093114
1103	22-23	0.21137199508721238
1103	24-25	0.20499222237872772
1103	26-27	0.16640827227868726
1103	28-29	0.2159119362529509
1103	30-31	0.13471093505571474
1103	32-33	0.15926213445943915
1103	34-35	0.18017796887995985
1103	36-37	0.2102675425937761
1103	38-39	0.26544939617042473
1103	40-41	0.11493711100775528
1103	42-43	0.07669565613129237
1103	44-45	0.2022544181441006
1103	46-47	-0.033508871905802096
1103	48-49	-0.024184462513737515
1103	50-51	0.2266992243988355
1103	52-53	-0.044656684022264415
1103	54-55	0.0508053624727296
1103	56-57	-0.11080052427110587
1103	58-59	-0.14848702231483202
1103	60-61	-0.054953478534038425
1103	62-63	-0.08441041925087234
1103	64-65	0.020155529876390688
1103	66-67	0.030197833655286388
1103	68-69	0.03850245810253483
1103	70-71	0.06805754576885903
1103	72-73	-0.13365743096446892
1103	74-75	0.00798709188523361
1103	76-77	-0.01900395834758939
1103	78-79	0.055296787391263535
1103	80-81	0.13547264121758218
1103	82-83	-0.04014274578192811
1103	84-85	-0.09871544618857087
1103	86-87	-0.08180566724986704
1103	88-89	-0.1271523519549973
1103	90-91	-0.12185018498283995
1103	92-93	-0.12578736367573384
1103	94-95	-0.1736539165990436
1103	96-97	-0.13531045340153725
1103	98-99	-0.08435335187630955
1103	100-101	-0.17392771604256296
1104	1	0.14840860080976626
1104	2	0.10404380734455287
1104	3	0.14013564437469483
1104	4	0.1655968403304655
1104	5	0.13656379061215773
1104	6	-0.018260452334708077
1104	7	-0.017275150091137448
1104	8	0.08645492629599971
1104	9	0.111504068498121
1104	10-11	0.1036680444491509
1104	12-13	0.13464412252093183
1104	14-15	0.16358516339484908
1104	16-17	0.17161347483641975
1104	18-19	0.17931743066453976
1104	20-21	0.14787781928124133
1104	22-23	-0.05360489575137706
1104	24-25	0.0909473917246757
1104	26-27	0.09176931795424537
1104	28-29	0.0681049285256492
1104	30-31	0.13530614983662304
1104	32-33	0.03229533779208538
1104	34-35	0.20037260621160868
1104	36-37	0.27797514735437545
1104	38-39	0.33003054961413625
1104	40-41	0.19934849884439387
1104	42-43	0.29387700989170895
1104	44-45	0.24314308570230736
1104	46-47	0.17634385615028947
1104	48-49	0.4082408243923723
1104	50-51	0.44870944166971327
1104	52-53	0.4349311559705811
1104	54-55	0.5085325232912368
1104	56-57	0.5736775891708206
1104	58-59	0.5432113052085725
1104	60-61	0.5586961742689951
1104	62-63	0.5185155754782258
1104	64-65	0.4212211811574704
1104	66-67	0.4835570552178723
1104	68-69	0.3111891638999751
1104	70-71	0.3041577768604782
1104	72-73	0.44804786287999576
1104	74-75	0.3036074022082573
1104	76-77	0.24435305301674504
1104	78-79	0.2854644547088725
1104	80-81	0.39084216398261873
1104	82-83	0.32607871048692516
1104	84-85	0.30000781460923065
1104	86-87	0.14699741247953568
1104	88-89	0.08292769079415052
1104	90-91	0.1485172115155322
1104	92-93	0.14422472247163398
1104	94-95	0.16241442049255994
1104	96-97	0.1548053442002626
1104	98-99	0.13204184276101927
1104	100-101	0.14970196104467703
1105	1	0.012535027963110679
1105	2	0.09530972781310965
1105	3	0.03818660306557575
1105	4	0.017565998648024106
1105	5	0.07313651194871795
1105	6	0.09921760033887495
1105	7	0.048064893308833234
1105	8	0.104159212867728
1105	9	0.17944679108786943
1105	10-11	0.1933404340199587
1105	12-13	0.16634786824194947
1105	14-15	0.1748282626013875
1105	16-17	0.1473965541238158
1105	18-19	0.18019730244333942
1105	20-21	0.15857742947297737
1105	22-23	0.1103638932047204
1105	24-25	0.056800583733277676
1105	26-27	0.11084815684009897
1105	28-29	0.15415427785585223
1105	30-31	0.12864445196111518
1105	32-33	0.2875359833831652
1105	34-35	0.23077303361559132
1105	36-37	0.14146508555229076
1105	38-39	0.0726047352050827
1105	40-41	0.034981327163862375
1105	42-43	0.1433932123281707
1105	44-45	0.1690208138654583
1105	46-47	0.29237876080885883
1105	48-49	0.23390682928641837
1105	50-51	0.24538650762051617
1105	52-53	0.2023660065440751
1105	54-55	0.13216110395384817
1105	56-57	0.20014959355038542
1105	58-59	0.25404283020972684
1105	60-61	0.19358434348931297
1105	62-63	0.182105109357245
1105	64-65	0.24355699668779351
1105	66-67	0.2826217792191059
1105	68-69	0.17081070031175472
1105	70-71	0.36929467145395023
1105	72-73	0.32888393894207724
1105	74-75	0.30534669897040345
1105	76-77	0.2486805183396399
1105	78-79	0.15382666639256826
1105	80-81	0.38303214769113936
1105	82-83	0.31597205736350986
1105	84-85	0.288676992054441
1105	86-87	0.2886251093129175
1105	88-89	0.27781137491095365
1105	90-91	0.4262170129837486
1105	92-93	0.17131210943713882
1105	94-95	0.28617623511267
1105	96-97	0.34332347918869033
1105	98-99	0.23226609793702124
1105	100-101	0.3103669435989431
1106	1	-0.07630431367077506
1106	2	0.02162683581220648
1106	3	0.024337866938111574
1106	4	0.007923490443495496
1106	5	-0.09224164773262089
1106	6	-0.28667813330844893
1106	7	-0.13154154004036656
1106	8	-0.07493240223360687
1106	9	0.05202614324359445
1106	10-11	0.05226332401670675
1106	12-13	-0.09965220691399423
1106	14-15	-0.13770837592355178
1106	16-17	-0.10691176062929486
1106	18-19	-0.07398888389330693
1106	20-21	-0.014072694534341679
1106	22-23	-0.018971978729545924
1106	24-25	-0.03720009267026114
1106	26-27	-0.06510193961003807
1106	28-29	-0.07509766993581479
1106	30-31	0.06303644033699385
1106	32-33	-0.05783879428048522
1106	34-35	-0.03655103883474453
1106	36-37	0.005192945862432907
1106	38-39	-0.09811137572510376
1106	40-41	0.06274330827466912
1106	42-43	0.06799801729848554
1106	44-45	0.06057641674142644
1106	46-47	-0.25724859094603403
1106	48-49	-0.05419848924955062
1106	50-51	-0.1206298513242885
1106	52-53	-0.12383335713555965
1106	54-55	-0.08190934222196944
1106	56-57	-0.06982811018486501
1106	58-59	-0.13907476379784356
1106	60-61	-0.30867534536506014
1106	62-63	-0.22470965628145478
1106	64-65	-0.1606273107501366
1106	66-67	0.07159264376303298
1106	68-69	-0.08543225381806252
1106	70-71	-0.2350474634760289
1106	72-73	-0.1938926975787112
1106	74-75	-0.2103464893954552
1106	76-77	-0.1086741175297874
1106	78-79	-0.1311020607161808
1106	80-81	-0.03799398152698785
1106	82-83	0.021553639145707848
1106	84-85	-0.09703648841754386
1106	86-87	-0.238488025441697
1106	88-89	0.17946731097829627
1106	90-91	0.21225178513596177
1106	92-93	0.01962047429421432
1106	94-95	-0.037557387153526633
1106	96-97	-0.5067798602406342
1106	98-99	-0.2333581974759582
1106	100-101	-0.10180957267231605
1107	1	0.10899289079444685
1107	2	0.17815770118765073
1107	3	0.1427569109224649
1107	4	0.1558817005379538
1107	5	0.15905116783841322
1107	6	0.2250526175741001
1107	7	0.1758671041729727
1107	8	0.19697390441652374
1107	9	0.09422154444577302
1107	10-11	0.09771461683227756
1107	12-13	0.10047659758938465
1107	14-15	0.10114764926488107
1107	16-17	0.11457235348195383
1107	18-19	0.0764857845543574
1107	20-21	0.15066110949199896
1107	22-23	0.13750641073710312
1107	24-25	0.19108680743469364
1107	26-27	0.0977761254600864
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2316	44-45	-0.5719596117390466
2316	46-47	-0.9849614242736635
2316	48-49	-0.9205901641887877
2316	50-51	-0.6461056668858518
2316	52-53	-0.5580715387670239
2316	54-55	-0.5751931542234772
2316	56-57	-0.5431464958350816
2316	58-59	-0.5247686329656815
2316	60-61	-0.4942040483397605
2316	62-63	-0.6362943959521488
2316	64-65	-0.5107822075426611
2316	66-67	-0.46094937520111756
2316	68-69	-0.4940705312972682
2316	70-71	-0.9896438760320834
2316	72-73	-0.6223996978867916
2316	74-75	-0.5120058533772394
2316	76-77	-0.3968566047786197
2316	78-79	-0.603828244264605
2316	80-81	-0.6828832431570859
2316	82-83	-0.5591398877087812
2316	84-85	-0.5353901323431884
2316	86-87	-0.5839600755563765
2316	88-89	-0.5268945826991853
2316	90-91	-0.4681473522343893
2316	92-93	-0.5217643543779502
2316	94-95	-0.5731845052174052
2316	96-97	-0.5374006455074678
2316	98-99	-0.506588437437621
2316	100-101	-0.48912343978627604
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	310.0
3	99.0
4	121.0
5	148.0
6	144.0
7	192.0
8	372.0
9	341.0
10	287.0
11	364.0
12	385.0
13	547.0
14	616.0
15	683.0
16	852.0
17	1039.0
18	1347.0
19	1867.0
20	2446.0
21	3261.0
22	4215.0
23	4450.0
24	4320.0
25	3848.0
26	3532.0
27	3536.0
28	3951.0
29	4539.0
30	5524.0
31	6869.0
32	8661.0
33	11909.0
34	18321.0
35	33066.0
36	74302.0
37	202190.0
38	356325.0
39	143413.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49423591460125	18.876911727463913	13.4189229363253	24.209929421609537
2	15.362055806956432	20.889889353022998	39.86606066145902	23.881994178561555
3	17.811238899563413	25.890980361930666	30.7964786910156	25.501302047490327
4	12.710704394412417	17.61784414454594	38.70992252927864	30.961528931762995
5	13.475079645549764	37.79120748381752	35.309637221681214	13.4240756489515
6	30.327728019666324	37.43738628203378	18.797772559662008	13.437113138637896
7	26.62404621781157	32.77132803497183	23.13226146907245	17.47236427814415
8	25.452251616955166	34.58212589662505	21.429862159392666	18.535760327027116
9	26.492544302807193	15.419569593669186	20.67574521161663	37.41214089190699
10-11	23.9604430362321	25.95189244135783	29.91661958553183	20.17104493687824
12-13	25.111790128554517	24.05918462851323	28.95240454758169	21.876620695350564
14-15	22.623184885156338	25.370266627225913	26.53712912055376	25.469419367063995
16-17	22.087386257963388	28.122740919494287	27.281076000905145	22.50879682163718
18-19	22.105633399679427	27.47553854825357	28.82201926972328	21.59680878234372
20-21	23.12911202318146	26.7733994486476	28.473586168629282	21.623902359541656
22-23	22.97043509677078	26.636555795066897	27.999310471688034	22.393698636474284
24-25	22.572288734859626	26.844202760729264	28.133550098928247	22.449958405482867
26-27	22.46817927220284	26.96619240624683	28.2075977224915	22.35803059905883
28-29	22.51403531587451	26.887521298192326	28.322793529317114	22.27564985661605
30-31	22.874664964690048	26.706096526545707	28.127147794733244	22.292090714031
32-33	22.664009436329316	26.907214391394806	28.056977518132896	22.371798654142978
34-35	22.883063960404993	26.96263325773471	27.92477918549741	22.22952359636289
36-37	22.899728549093805	26.801807761599513	27.92294851694308	22.37551517236361
38-39	22.760677529402294	27.007479206350432	27.9697059605337	22.262137303713573
40-41	22.752377818673448	26.89077714450116	28.044288577927475	22.312556458897923
42-43	22.688315186110696	26.83170918031541	27.949403314472853	22.53057231910104
44-45	22.856241285197886	26.89825736999663	27.684709748903558	22.56079159590192
46-47	22.89421450097813	27.00395216862586	27.505408346698157	22.596424983697855
48-49	22.773746919920125	27.039189195145923	27.634157038891654	22.55290684604229
50-51	22.859145553855853	27.139256382336214	27.397646768384348	22.603951295423585
52-53	22.924055000622854	27.174113151886694	27.25436740795289	22.64746443953757
54-55	22.874428605391724	27.088862031817722	27.323128994659907	22.71358036813065
56-57	22.886386029567852	27.235108694633336	27.31500243430546	22.563502841493356
58-59	22.84443753726844	27.07146392084376	27.43282312081643	22.651275421071368
60-61	22.94692013195494	27.238544965080486	27.39394507761731	22.420589825347264
62-63	22.786845724518432	27.184494942000022	27.50468507005952	22.523974263422026
64-65	22.85862632721109	27.0141081787047	27.46777255865717	22.65949293542704
66-67	22.774587370174046	27.19011639216793	27.309313522619625	22.725982715038402
68-69	22.816984146315253	27.14926291855706	27.39608642294384	22.637666512183845
70-71	22.952675345030258	26.90230255308338	27.336073772520038	22.808948329366327
72-73	22.843696930579068	27.062021737896245	27.278199102549756	22.81608222897493
74-75	22.86182814368947	27.162094378207353	27.205750224618146	22.770327253485025
76-77	22.81776104250556	27.180144598924823	27.197560843872182	22.804533514697436
78-79	22.83196233239238	27.094543930839805	27.167265790402258	22.90622794636556
80-81	22.905364278355094	27.193718727874632	27.04830869088475	22.852608302885518
82-83	22.984770979642388	27.16988151036205	27.113308062019954	22.73203944797561
84-85	23.018904142231165	27.05881509330721	27.135872419103595	22.786408345358026
86-87	22.86747536907091	27.178225822911052	27.12841510913952	22.825883698878513
88-89	22.858876450357574	27.10993069173127	27.129512415088406	22.901680442822748
90-91	23.047330052259383	27.15028968975713	27.07288471896868	22.729495539014806
92-93	23.025179233499106	27.181147381035593	27.068034313168855	22.72563907229645
94-95	22.92746271058831	27.13834699683959	27.171031382521953	22.763158910050148
96-97	22.945102058810562	27.106919278755015	27.18076312657056	22.767215535863862
98-99	23.014872199612874	27.100648591925008	27.064823918976717	22.819655289485404
100-101	22.927618032175033	27.14981219929974	27.137132275313125	22.785437493212104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	180.0
2	124.0
3	134.0
4	135.5
5	146.5
6	171.5
7	248.5
8	436.0
9	536.5
10	431.5
11	372.0
12	417.0
13	436.5
14	464.5
15	527.5
16	574.0
17	596.5
18	647.0
19	705.5
20	768.5
21	937.0
22	1201.0
23	1542.5
24	1952.5
25	2613.0
26	3702.0
27	5171.0
28	6773.5
29	8890.0
30	11244.5
31	13194.0
32	15503.5
33	17838.0
34	19548.5
35	20979.5
36	23241.0
37	25436.5
38	26282.0
39	27160.0
40	28158.0
41	28615.0
42	28996.5
43	30014.0
44	31315.5
45	32377.5
46	33235.0
47	33739.0
48	34242.5
49	34671.0
50	34406.0
51	33574.0
52	32453.5
53	31646.5
54	30617.5
55	28993.0
56	26698.0
57	23935.5
58	21660.0
59	19266.0
60	16400.0
61	13582.0
62	10773.5
63	7947.0
64	5610.5
65	3937.5
66	2861.5
67	2119.5
68	1508.5
69	1078.5
70	811.0
71	592.5
72	401.0
73	258.5
74	168.0
75	123.5
76	114.0
77	83.0
78	41.0
79	29.0
80	23.5
81	23.5
82	21.5
83	16.5
84	16.5
85	14.0
86	11.0
87	8.5
88	6.0
89	3.5
90	2.5
91	3.5
92	4.5
93	3.5
94	5.0
95	4.5
96	1.0
97	1.5
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0444633254605366
2	0.06504412214647803
3	0.047214768867748017
4	0.05822054249659371
5	0.09266861395488073
6	0.0732984523681123
7	0.10037265549507272
8	0.11490027668514904
9	0.11589079631174515
10-11	0.06680504592709335
12-13	0.048315346230632585
14-15	0.10163831946238998
16-17	0.05211233813258436
18-19	0.027074203126960402
20-21	0.03323743635911399
22-23	0.08221312900747732
24-25	0.18087988959007895
26-27	0.1824757267662616
28-29	0.13867274772345572
30-31	0.157767764969503
32-33	0.20971501649765467
34-35	0.17950416788647325
36-37	0.18005445656791552
38-39	0.21648356727939477
40-41	0.15710741855177224
42-43	0.08887162205292898
44-45	0.07439902973099688
46-47	0.08342376410665035
48-49	0.1287675514574946
50-51	0.13933309414118647
52-53	0.16475643122382003
54-55	0.23877025887780728
56-57	0.19639803040675138
58-59	0.14802765530797457
60-61	0.24696956023129735
62-63	0.2506564943969607
64-65	0.2682657322031138
66-67	0.14235968188911904
68-69	0.14147921999881138
70-71	0.1473122800220996
72-73	0.16382094046536813
74-75	0.16717770142216606
76-77	0.15573169684816654
78-79	0.19084011472418433
80-81	0.2812525450851517
82-83	0.2984765808142952
84-85	0.23618390207502857
86-87	0.24020100944955725
88-89	0.23733950830605735
90-91	0.1866028918770787
92-93	0.17135989540112742
94-95	0.1599138908271279
96-97	0.14241471075726325
98-99	0.15606187005703193
100-101	0.18363133299729037
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	908614.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.48958144825467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3252743377125	47.93205833065783
2	15.3060293822742	19.74158856995102
3	5.104549557525589	9.875707933400964
4	2.2188394904555166	5.723681201613397
5	1.081645417312991	3.487743011896878
6	0.6134103430139057	2.3735145766198262
7	0.36184348426876356	1.633459439518948
8	0.24358318442860974	1.2566862089307251
9	0.1586694855879842	0.9209275842761079
>10	0.5678021758935874	5.725449063984684
>50	0.01578102538463078	0.6987497615284884
>100	0.0020576930270640188	0.19761204663801285
>500	1.714743715325395E-4	0.09023581326576566
>1k	3.42948743065079E-4	0.3425864577173309
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1764	0.19414184681283803	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1334	0.14681702020880152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	5.5028868144228464E-5	0.0
22-23	0.0	0.0	0.0	1.1005773628845693E-4	0.0
24-25	0.0	0.0	0.0	1.1005773628845693E-4	0.0
26-27	0.0	0.0	0.0	4.402309451538277E-4	0.0
28-29	0.0	0.0	0.0	9.35490758451884E-4	0.0
30-31	0.0	0.0	0.0	0.0018709815169037677	0.0
32-33	0.0	0.0	0.0	0.004787511528547876	0.0
34-35	0.0	0.0	0.0	0.008309359089778498	0.0
36-37	0.0	0.0	0.0	0.013812245904201343	0.0
38-39	0.0	0.0	0.0	0.020800912158518356	0.0
40-41	0.0	0.0	0.0	0.027624491808402686	0.0
42-43	0.0	0.0	0.0	0.03428298485385433	0.0
44-45	0.0	0.0	0.0	0.04154679544889249	0.0
46-47	0.0	0.0	0.0	0.052057309264440126	0.0
48-49	0.0	0.0	0.0	0.06108204364009359	0.0
50-51	0.0	0.0	0.0	0.07021683575203552	0.0
52-53	0.0	0.0	0.0	0.07973682994098705	0.0
54-55	0.0	0.0	0.0	0.09063254583354427	0.0
56-57	0.0	0.0	0.0	0.10295901229785145	0.0
58-59	0.0	0.0	0.0	0.11682628707019703	0.0
60-61	0.0	0.0	0.0	0.12992315768852342	0.0
62-63	0.0	0.0	0.0	0.14197447981210942	0.0
64-65	0.0	0.0	0.0	0.1543009462764166	0.0
66-67	0.0	0.0	0.0	0.16849839425762755	0.0
68-69	0.0	0.0	0.0	0.1853922567779057	0.0
70-71	0.0	0.0	0.0	0.2033866966610684	0.0
72-73	0.0	0.0	0.0	0.22110599220350996	0.0
74-75	0.0	0.0	0.0	0.24190690436202833	0.0
76-77	0.0	0.0	0.0	0.26441371143301773	0.0
78-79	0.0	0.0	0.0	0.28972699077936287	0.0
80-81	0.0	0.0	0.0	0.3172414248514771	0.0
82-83	0.0	0.0	0.0	0.342994935142976	0.0
84-85	0.0	0.0	0.0	0.3717750331824075	0.0
86-87	0.0	0.0	0.0	0.40418703651935806	0.0
88-89	0.0	0.0	0.0	0.43863510797764504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	970	0.0	61.626236	1
GTATCAA	1850	0.0	42.057064	1
TCAACGC	2180	0.0	34.83164	4
ATCAACG	2230	0.0	34.263477	3
CAACGCA	2225	0.0	34.12718	5
AACGCAG	2315	0.0	32.994514	6
TATCAAC	2390	0.0	31.966166	2
GTACATG	2845	0.0	30.349808	1
TACATGG	2790	0.0	30.104513	2
ACGCAGA	2580	0.0	29.605543	7
ACACCGC	100	4.9930866E-5	28.465452	6
CGCAGAG	2755	0.0	27.552767	8
ACATGGG	2965	0.0	27.530506	3
CGCGAAT	35	0.008347754	27.172836	78-79
CATGGGG	2035	0.0	25.186615	4
TAACGCA	100	0.0016618901	23.729055	4
GCAGAGT	3285	0.0	22.529734	9
CAGAGTA	2970	0.0	21.56236	10-11
ATGGGGG	915	0.0	20.746714	5
GTATAGG	280	1.5988917E-9	20.332464	1
>>END_MODULE