FastQCFastQC Report
Fri 10 Feb 2017
SRR2031404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908614
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.23695430622904776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.1903998837790305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.10356432984743796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10950.055.1341861
GTATCAA21950.046.345831
TCAACGC25500.039.4882974
CAACGCA26150.038.3251155
ATCAACG26550.038.105513
AACGCAG26700.037.889356
TATCAAC28500.035.831612
ACGCAGA31050.032.428227
CGCAGAG32600.030.7406948
TACATGG28850.028.4821642
GTACATG28600.028.422451
TTAGACG856.4079376E-427.9398334
ACATGGG30000.026.282073
GCAGAGT38750.025.8618499
GTATAGA3550.024.1032471
CATGGGG21150.022.9066984
CAGAGTA34750.022.68830710-11
AGAGTAC32800.022.00992610-11
GAGTACT22200.021.39536912-13
GTACTTT23350.020.74960914-15
Produced by FastQC (version 0.11.5)