FastQCFastQC Report
Fri 10 Feb 2017
SRR2031403_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031403_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034482
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.14384010548274404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.10691341173650194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.052.2634961
GTATCAA21700.043.737561
ATCAACG24450.038.2451863
TCAACGC24750.037.589824
CAACGCA25350.037.0746155
AACGCAG26150.035.926496
TATCAAC26950.034.8718342
ACGCAGA29050.031.850027
CGCAGAG30100.030.896618
GTACATG29550.029.0673471
TACATGG29700.028.1270772
ACATGGG30150.027.078873
GTATAGG2155.820766E-1126.4866541
GCAGAGT36600.024.5020249
CATGGGA13600.023.733374
CATGGGG18550.022.0061444
GAGTACT19650.021.4885312-13
AGAGTAC30150.021.47951910-11
CAGAGTA32850.021.37497310-11
GTATAGC4600.020.6327191
Produced by FastQC (version 0.11.5)