FastQCFastQC Report
Fri 10 Feb 2017
SRR2031403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034482
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19130.18492346894387723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13830.1336900980394052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.060.3472061
GTATCAA21750.046.562561
CAACGCA26250.037.817475
TCAACGC26300.037.7455754
ATCAACG26650.037.428083
TATCAAC27350.036.645582
AACGCAG27450.036.16256
ACGCAGA31000.031.8680977
CGCAGAG32550.029.912828
GTACATG30500.029.1513251
CGACGCG350.00839084627.14434856-57
TACATGG31750.027.0789182
ACATGGG30950.026.8567123
GCAGAGT38950.024.3880489
CATGGGG18750.023.812344
CATGGGA13250.022.9424384
AGAGTAC32350.022.02274710-11
GAGTACT21500.021.76019312-13
CAGAGTA36150.021.0216210-11
TGGACCG1150.003723929620.6513125
Produced by FastQC (version 0.11.5)