FastQCFastQC Report
Fri 10 Feb 2017
SRR2031402_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031402_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208258
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.24696712126052547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.1758730337394828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.10751015097768854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.056.5378381
GTATCAA26350.045.5788421
TCAACGC32900.035.7861634
ATCAACG32850.035.696113
CAACGCA33450.035.197755
AACGCAG34800.033.953266
TATCAAC35200.033.3129922
TATGCCG1151.0413896E-733.0256585
ACGCAGA39800.029.568557
CGCAGAG40550.028.9046348
GTACATG34850.027.5151521
TACATGG35600.026.8043582
ACATGGG36900.025.4740623
GTACGGA1151.2872317E-424.767191
GTACCGT1601.8758528E-623.7263746
GCAGAGT47200.023.625849
TACCGTA1050.002209023822.5965487
AGAGTAC40900.022.50815210-11
GTATAGA4900.021.3132631
CAGAGTA43900.020.2133610-11
Produced by FastQC (version 0.11.5)