FastQCFastQC Report
Fri 10 Feb 2017
SRR2031402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208258
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33180.274610223975343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.21113040426796265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.13151164734684148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.061.5019071
GTATCAA32700.049.865391
TCAACGC40500.039.638664
ATCAACG40500.039.5213853
CAACGCA40800.039.230795
AACGCAG41850.038.3599936
TATCAAC42200.038.1543922
ACGCAGA46600.034.2460637
CGCAGAG47450.033.5324978
GCAGAGT54300.029.0399289
TACATGG38800.024.8497242
GTACATG40450.024.7980081
ACATGGG39700.024.5256563
AGAGTAC50000.023.98549510-11
GTACCGT1601.8610408E-623.7480136
TAGTACT4600.022.7154924
CAGAGTA53050.022.2036110-11
GAGTACT34450.021.92260412-13
CATGGGG26950.021.3247454
GTATAGT4250.021.2528671
Produced by FastQC (version 0.11.5)