FastQCFastQC Report
Fri 10 Feb 2017
SRR2031401_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031401_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541415
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17340.32027188016586167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.2644921178763056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.1390800033246216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.066.606931
GTATCAA14100.047.1384471
TCAACGC17850.037.2354134
TATCAAC18150.036.619952
ATCAACG18250.036.4192963
CAACGCA18400.036.12245
AACGCAG19150.034.9426576
ACGCAGA22050.030.3470277
GTACATG18550.030.199831
GTCCGAT650.005856321729.2154771
TACATGG19000.028.9848272
CGCAGAG23500.028.67658
ACATGGG19800.027.5739523
GTATAAG2059.604264E-1025.4744721
GCAGAGT25900.025.4718729
GTGGTAT2800.025.4331191
GTCTAAG1901.1947122E-824.9869211
GTTCGAA950.001231449224.9799869
TCTATAC1151.2850815E-424.7696463
CATGGGG12950.024.1958314
Produced by FastQC (version 0.11.5)