##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031401_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541415
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.909062364360054	34.0	33.0	34.0	31.0	34.0
2	32.994820978362256	34.0	33.0	34.0	31.0	34.0
3	33.16391123260345	34.0	34.0	34.0	31.0	34.0
4	36.117754402814846	37.0	37.0	37.0	35.0	37.0
5	36.09561057599069	37.0	37.0	37.0	35.0	37.0
6	36.17095204233352	37.0	37.0	37.0	35.0	37.0
7	36.21418505213192	37.0	37.0	37.0	35.0	37.0
8	36.30191073391022	37.0	37.0	37.0	35.0	37.0
9	38.26044716160431	39.0	39.0	39.0	37.0	39.0
10-11	38.35027751355245	39.0	39.0	39.0	37.5	39.0
12-13	38.401786984106465	39.0	39.0	39.0	38.0	39.0
14-15	40.044796505453306	41.0	40.0	41.0	38.0	41.0
16-17	40.04523794132043	41.0	40.0	41.0	38.0	41.0
18-19	40.05892984124932	41.0	40.0	41.0	38.5	41.0
20-21	39.997118661285704	41.0	40.0	41.0	38.5	41.0
22-23	39.93446893787575	41.0	40.0	41.0	38.0	41.0
24-25	39.821085488950246	41.0	40.0	41.0	38.0	41.0
26-27	39.72995576406269	41.0	40.0	41.0	38.0	41.0
28-29	39.65384963475338	41.0	40.0	41.0	38.0	41.0
30-31	39.49005661091769	41.0	40.0	41.0	37.0	41.0
32-33	39.30093274105815	41.0	40.0	41.0	36.5	41.0
34-35	39.203967381768145	41.0	40.0	41.0	36.0	41.0
36-37	39.077674242494204	41.0	39.5	41.0	35.0	41.0
38-39	38.89882068284034	41.0	39.0	41.0	35.0	41.0
40-41	38.769377464606634	41.0	39.0	41.0	35.0	41.0
42-43	38.944001366788875	41.0	39.0	41.0	35.0	41.0
44-45	38.90794861612626	41.0	39.0	41.0	35.0	41.0
46-47	38.71925325304988	41.0	39.0	41.0	35.0	41.0
48-49	38.505763600934586	41.0	39.0	41.0	35.0	41.0
50-51	37.468280339480806	40.0	38.0	40.5	33.5	40.5
52-53	37.50546807901517	39.5	38.0	40.5	33.5	41.0
54-55	37.897378166471185	40.0	38.0	41.0	34.0	41.0
56-57	37.87998485450163	40.0	37.0	41.0	34.0	41.0
58-59	37.865570772882165	40.0	37.0	41.0	34.0	41.0
60-61	37.64789117405317	40.0	36.5	41.0	34.0	41.0
62-63	37.38644939648883	39.5	36.0	41.0	34.0	41.0
64-65	37.10613392683986	39.0	35.5	41.0	33.5	41.0
66-67	36.831963466102714	39.0	35.0	41.0	33.0	41.0
68-69	36.5082820017916	37.5	35.0	41.0	33.0	41.0
70-71	36.1492404163165	37.0	35.0	40.5	33.0	41.0
72-73	35.79390393690608	36.5	35.0	39.0	32.5	41.0
74-75	35.44492117876305	36.0	35.0	39.0	32.0	41.0
76-77	35.09528180785534	36.0	35.0	38.5	32.0	40.5
78-79	34.73346693386773	35.0	35.0	37.0	31.5	39.0
80-81	34.38403165778561	35.0	35.0	37.0	31.0	39.0
82-83	34.09976358246447	35.0	35.0	36.5	31.0	39.0
84-85	33.827719956041115	35.0	35.0	36.0	31.0	37.0
86-87	33.608820405788535	35.0	35.0	36.0	30.0	37.0
88-89	33.43735304710804	35.0	35.0	35.5	30.0	37.0
90-91	33.24369568630348	35.0	35.0	35.0	30.0	36.0
92-93	33.17968748557022	35.0	35.0	35.0	30.0	36.0
94-95	33.09202552570579	35.0	34.0	35.0	30.0	36.0
96-97	33.00258304627688	35.0	34.0	35.0	29.0	36.0
98-99	32.92018322359004	35.0	34.0	35.0	29.0	35.5
100-101	32.568043921945275	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13966407366956446
1101	2	0.20252755828396118
1101	3	0.16021332533407673
1101	4	0.08691076431993139
1101	5	0.22463737207917944
1101	6	0.19712841421163319
1101	7	0.25954116383318393
1101	8	0.2655105108680118
1101	9	0.323836348927216
1101	10-11	0.23843644830404287
1101	12-13	0.1148203240213661
1101	14-15	0.23383848295535614
1101	16-17	0.2836537739843692
1101	18-19	0.33996972908631307
1101	20-21	0.33247597077540547
1101	22-23	0.17854779963906964
1101	24-25	0.20975897586538395
1101	26-27	0.2489385460684943
1101	28-29	0.4999330149098853
1101	30-31	0.2853591829361264
1101	32-33	0.20523366983307767
1101	34-35	0.19112524689819566
1101	36-37	0.15568069027487041
1101	38-39	0.3529546946292257
1101	40-41	0.4029412994360442
1101	42-43	0.3928785930062233
1101	44-45	0.23629137925206578
1101	46-47	0.3161199360143385
1101	48-49	0.2621722661691237
1101	50-51	0.2498744550955081
1101	52-53	0.2692331007149278
1101	54-55	-0.07609525676397766
1101	56-57	0.018823060893041088
1101	58-59	0.17160228864061366
1101	60-61	0.21633986461371535
1101	62-63	0.04647050540265241
1101	64-65	0.19654585200977692
1101	66-67	0.25174908261938356
1101	68-69	0.15629935266149886
1101	70-71	0.20740554101683273
1101	72-73	0.23996027324302105
1101	74-75	0.23224160536309313
1101	76-77	0.3888956630654832
1101	78-79	0.4699189757051343
1101	80-81	0.2978502922440569
1101	82-83	0.14925562521727898
1101	84-85	0.4281092473244783
1101	86-87	0.26183137795350575
1101	88-89	0.19588791526123117
1101	90-91	0.033486195587954626
1101	92-93	0.09518746544847545
1101	94-95	0.04106692728294803
1101	96-97	0.19339851391996632
1101	98-99	0.2768068170433935
1101	100-101	0.09297303915776212
1102	1	-0.015780762269656634
1102	2	-0.15245115418759525
1102	3	-0.08545576370651986
1102	4	-0.4421118445033869
1102	5	-0.2210610790696137
1102	6	-0.15624455796777426
1102	7	-0.13036010607800108
1102	8	-0.08280667330581082
1102	9	-0.13566229307528488
1102	10-11	-0.0868819470567388
1102	12-13	-0.12154464698355127
1102	14-15	-0.06933445986194187
1102	16-17	-0.17506493701908
1102	18-19	-0.12435989728086128
1102	20-21	-0.08533306541339414
1102	22-23	-0.0588173181763878
1102	24-25	0.01872634722264621
1102	26-27	0.05135449703377759
1102	28-29	-0.0134083930032034
1102	30-31	0.08167332079121792
1102	32-33	0.031240239604784392
1102	34-35	-0.013184560045942817
1102	36-37	-0.07220138415807043
1102	38-39	0.1280959997749349
1102	40-41	-0.10967064360146139
1102	42-43	-0.1667494257855182
1102	44-45	0.0689824164685362
1102	46-47	0.010198883382763313
1102	48-49	0.06645087587329357
1102	50-51	-0.03727778743551369
1102	52-53	-0.0872871224371039
1102	54-55	0.09484267907981803
1102	56-57	-0.08215965216575682
1102	58-59	-0.19464872068185457
1102	60-61	-0.005789071743919294
1102	62-63	-0.051713631718392605
1102	64-65	-0.12710716127657662
1102	66-67	-0.02690191202202641
1102	68-69	-0.17320773094710518
1102	70-71	-0.20415487152079237
1102	72-73	-0.23332479612495405
1102	74-75	-0.21203920649919183
1102	76-77	-0.13656220230924987
1102	78-79	-0.04756065433229395
1102	80-81	-0.09220237603752679
1102	82-83	-0.21502720528994956
1102	84-85	-0.09358201010780931
1102	86-87	-1.1074199071003932E-4
1102	88-89	-0.18988889601381942
1102	90-91	-0.06452819184507064
1102	92-93	-0.032656988783337226
1102	94-95	-0.06224640311418028
1102	96-97	-0.17085678961083062
1102	98-99	-0.3060035037376352
1102	100-101	-0.4309937816599714
1103	1	-0.13092856646736095
1103	2	-0.10978523589661648
1103	3	-0.07994499730178717
1103	4	-0.23010891903479802
1103	5	-0.32927790391568834
1103	6	-0.3332524159124617
1103	7	-0.2363403081394324
1103	8	-0.15003292088209008
1103	9	-0.016985217183183465
1103	10-11	0.04783418043925991
1103	12-13	0.09695554011037189
1103	14-15	0.16473279189843737
1103	16-17	0.15517709448073447
1103	18-19	0.04902514200885122
1103	20-21	0.05035680089949324
1103	22-23	0.11751870250598984
1103	24-25	0.15282166307120093
1103	26-27	0.037369226427934166
1103	28-29	0.01715595029713768
1103	30-31	0.03517197711669695
1103	32-33	0.07763953204958796
1103	34-35	0.07594018057385199
1103	36-37	-0.054444470187263505
1103	38-39	0.021065520474323307
1103	40-41	-0.13891257305004956
1103	42-43	0.017316975547949198
1103	44-45	0.013355992003454276
1103	46-47	0.10369481834211314
1103	48-49	0.255245650850334
1103	50-51	0.4325873348558815
1103	52-53	0.3952493608775285
1103	54-55	0.49758360502463717
1103	56-57	0.4202725688091675
1103	58-59	0.44992385475101315
1103	60-61	0.332434216346698
1103	62-63	0.2193684514873766
1103	64-65	0.292127794671309
1103	66-67	0.17940740525524745
1103	68-69	0.16127367872054776
1103	70-71	0.2206971541961238
1103	72-73	0.10680344825372146
1103	74-75	0.3691425467409317
1103	76-77	0.3517486797535412
1103	78-79	0.16552231331746015
1103	80-81	0.2742891026847971
1103	82-83	0.20814522727119567
1103	84-85	0.1498144249025728
1103	86-87	0.3964858494982195
1103	88-89	0.4152663919407402
1103	90-91	0.4476924429135849
1103	92-93	0.30999277139627424
1103	94-95	0.49562940053925786
1103	96-97	0.2942757069024182
1103	98-99	0.10099380891331577
1103	100-101	0.38266067287620587
1104	1	0.16153462843485755
1104	2	0.2253294955595777
1104	3	0.2703704749649063
1104	4	0.28626895709917477
1104	5	0.19017621919697092
1104	6	0.16952562345094435
1104	7	0.21082985854292957
1104	8	0.26316512036472517
1104	9	0.2859544820979778
1104	10-11	0.30283308177962454
1104	12-13	0.24153914810860755
1104	14-15	0.28290540702227673
1104	16-17	0.27614344410562097
1104	18-19	0.22853827883906774
1104	20-21	0.28744362640095034
1104	22-23	0.2906439183141387
1104	24-25	0.34801424241012313
1104	26-27	0.32338564354190424
1104	28-29	0.3531809997368214
1104	30-31	0.3133133589166164
1104	32-33	0.3577898914682436
1104	34-35	0.3457474442835462
1104	36-37	0.4379276929166167
1104	38-39	0.4275499665929203
1104	40-41	0.48816703268809647
1104	42-43	0.548008817215397
1104	44-45	0.4123734763867901
1104	46-47	0.2466853698692475
1104	48-49	0.36340084437138387
1104	50-51	0.4355497920866327
1104	52-53	0.5103849668667948
1104	54-55	0.4346029414342283
1104	56-57	0.3807286695091747
1104	58-59	0.4835349920206795
1104	60-61	0.4334324271010104
1104	62-63	0.6225686900534697
1104	64-65	0.6272425261275032
1104	66-67	0.6066015854982041
1104	68-69	0.34493817945821803
1104	70-71	0.26207346081633176
1104	72-73	0.34040987698210046
1104	74-75	0.4073288166345179
1104	76-77	0.3532279464767143
1104	78-79	0.43595738253301874
1104	80-81	0.4456564630765456
1104	82-83	0.4170788996194972
1104	84-85	0.6000255245039128
1104	86-87	0.5735270021491274
1104	88-89	0.5065336173543002
1104	90-91	0.4373336520669895
1104	92-93	0.4675287917371733
1104	94-95	0.4407866658184716
1104	96-97	0.3624867751134886
1104	98-99	0.3966588118953922
1104	100-101	0.2915268561326343
1105	1	0.2831562500564786
1105	2	0.32378509401517874
1105	3	0.2549264595208953
1105	4	0.02565119648141234
1105	5	0.23650826552901805
1105	6	0.34520129912662156
1105	7	0.313146460859528
1105	8	0.31142766862727456
1105	9	0.23576143190492616
1105	10-11	0.20341223235877237
1105	12-13	0.24443490100436094
1105	14-15	0.22595560007247428
1105	16-17	0.2182283861905674
1105	18-19	0.23143403173482113
1105	20-21	0.3540459430032712
1105	22-23	0.2906439183141387
1105	24-25	0.32967447407035877
1105	26-27	0.2442350643913329
1105	28-29	0.18040107695689755
1105	30-31	0.23512803073128907
1105	32-33	0.2120369957153514
1105	34-35	0.18744628598239643
1105	36-37	0.21591997090888526
1105	38-39	0.15631444535739547
1105	40-41	-0.052373507852450985
1105	42-43	0.05862657783315939
1105	44-45	2.113142246287225E-4
1105	46-47	0.04591316909704801
1105	48-49	0.13270586367639936
1105	50-51	0.029179135715978077
1105	52-53	0.04223824872006787
1105	54-55	0.152749659580941
1105	56-57	0.19926148804199073
1105	58-59	0.13121838970408817
1105	60-61	0.1940501877187728
1105	62-63	0.29534861283339353
1105	64-65	0.4264703253553037
1105	66-67	0.2928950217916366
1105	68-69	0.26771810223814185
1105	70-71	0.23311593185879786
1105	72-73	0.14446393103615662
1105	74-75	0.26254117184686976
1105	76-77	0.27697312022188214
1105	78-79	0.2844129809886127
1105	80-81	0.21013522755530545
1105	82-83	0.12171210425270829
1105	84-85	0.13863556311395087
1105	86-87	0.12468530330743022
1105	88-89	0.31444867526936804
1105	90-91	0.3079900227233594
1105	92-93	0.25131257552095576
1105	94-95	0.07206079709260393
1105	96-97	0.23893465156136529
1105	98-99	0.20940012463670143
1105	100-101	0.18341874802452907
1106	1	-0.03719351146975214
1106	2	0.06149943650893164
1106	3	0.12028814473551819
1106	4	0.11909600683798516
1106	5	0.003726639365041251
1106	6	-0.31141776741891647
1106	7	-0.03547947600169721
1106	8	0.1387045253158945
1106	9	0.24559847483021002
1106	10-11	0.23290561755069206
1106	12-13	-0.054168324069451046
1106	14-15	-0.09643112488777916
1106	16-17	0.04271595148694729
1106	18-19	0.17398030700167766
1106	20-21	0.193963328876535
1106	22-23	0.2854556943759121
1106	24-25	0.305798707547531
1106	26-27	0.1739740207846978
1106	28-29	0.23613012533300548
1106	30-31	0.38380152039742654
1106	32-33	0.14149205476599747
1106	34-35	0.09803636660923587
1106	36-37	0.18102898748849583
1106	38-39	0.09021604653840853
1106	40-41	0.321849323171854
1106	42-43	0.2770536446058145
1106	44-45	0.16019811299818798
1106	46-47	-0.18189670485252663
1106	48-49	0.12757796792350717
1106	50-51	0.04852319090561963
1106	52-53	0.11911055169531437
1106	54-55	0.24712778245171307
1106	56-57	0.034302937054029314
1106	58-59	0.077352417639581
1106	60-61	-0.23165742640796338
1106	62-63	-0.26461050594925695
1106	64-65	-0.08162429762901979
1106	66-67	0.03432132204147109
1106	68-69	0.06987004409596409
1106	70-71	-0.10491708540215683
1106	72-73	0.03146569119967779
1106	74-75	-0.1518850716234894
1106	76-77	-0.08109595200159703
1106	78-79	0.05921851130884903
1106	80-81	0.22931959048967343
1106	82-83	0.24850420622863112
1106	84-85	0.26363556311395087
1106	86-87	-0.14319313185041693
1106	88-89	0.07161353445628293
1106	90-91	0.12281798093367513
1106	92-93	0.0916238689572495
1106	94-95	0.26250978074011755
1106	96-97	-0.08133703819562044
1106	98-99	-0.028033869231109065
1106	100-101	-0.012402992833976612
1107	1	0.09325062230807646
1107	2	0.1762799236580861
1107	3	0.17349951154915288
1107	4	0.18534641010715802
1107	5	0.11835590057592071
1107	6	-0.029065498357724096
1107	7	0.11528942388000729
1107	8	0.1527382923876246
1107	9	0.2867520934988832
1107	10-11	0.18490482445466228
1107	12-13	0.1687588724032878
1107	14-15	0.19811331537460575
1107	16-17	0.14630571920390878
1107	18-19	0.07942376866422762
1107	20-21	0.22948798206505217
1107	22-23	0.1188080709755468
1107	24-25	0.14888667399553412
1107	26-27	0.08951433425927036
1107	28-29	0.24770395047549698
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2316	68-69	-0.9807814823373917
2316	70-71	-1.6791561956032055
2316	72-73	-1.1121633110341236
2316	74-75	-0.875716977803684
2316	76-77	-0.9251088894550605
2316	78-79	-0.8361603047745447
2316	80-81	-0.8883228145736197
2316	82-83	-0.7906455517758317
2316	84-85	-0.9930100065062959
2316	86-87	-1.129571575632994
2316	88-89	-1.0485907656181794
2316	90-91	-1.0715881992599279
2316	92-93	-1.1970232829414797
2316	94-95	-0.9658479072717938
2316	96-97	-1.0137411707347113
2316	98-99	-1.0770961365431013
2316	100-101	-1.0710287374356184
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	181.0
3	69.0
4	67.0
5	84.0
6	71.0
7	109.0
8	252.0
9	254.0
10	210.0
11	234.0
12	265.0
13	324.0
14	385.0
15	402.0
16	542.0
17	641.0
18	897.0
19	1217.0
20	1624.0
21	2247.0
22	2984.0
23	3456.0
24	3287.0
25	2592.0
26	2409.0
27	2347.0
28	2513.0
29	2841.0
30	3343.0
31	4138.0
32	5359.0
33	7404.0
34	11366.0
35	20756.0
36	46924.0
37	121792.0
38	200554.0
39	87125.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94610216345266	18.896210446955866	13.604916736268692	23.55277065332279
2	15.64709209162032	21.151297732779604	39.76978481089863	23.431825364701446
3	17.6850609632151	26.43353180102967	31.095859527742974	24.785547708012256
4	12.778001604157502	17.87397383818708	38.51382569387204	30.834198863783374
5	13.335416231445723	37.816432587125846	35.15426695235648	13.693884229071958
6	29.98184923404507	37.40554947654818	18.815985982137576	13.796615307269178
7	26.23021742345807	33.33715426712025	23.277067001922795	17.15556130749889
8	25.286193729717738	34.399829883229636	21.659193239707474	18.65478314734516
9	26.703475922974228	15.134817291228279	20.838711108974383	37.32299567682311
10-11	24.022770749198312	25.95190788196915	29.72932011200547	20.296001256827065
12-13	25.27420846588321	24.160373723663653	29.021423879027658	21.54399393142548
14-15	22.639993713860477	25.43741824551997	26.87019584097916	25.052392199640394
16-17	21.95859351755516	28.21659770514282	27.491069498062366	22.333739279239655
18-19	21.885054710558283	27.257359020429277	29.4321094857808	21.42547678323164
20-21	23.08399386582414	26.382683886702512	29.18361879422796	21.349703453245386
22-23	22.81642729527114	26.3002908688262	28.764033935620663	22.119247900281998
24-25	22.269026515922505	26.59114038820939	28.94352645115926	22.196306644708844
26-27	22.314012884411305	26.77452095174036	28.817559659561397	22.093906504286938
28-29	22.359799838901946	26.599490071939254	28.91136740067084	22.129342688487963
30-31	22.50756837902109	26.541354843901317	28.815984564484072	22.135092212593523
32-33	22.42722013635611	26.829317951086157	28.629229662959617	22.114232249598125
34-35	22.766318652598077	26.711197664898073	28.598985100310394	21.92349858219346
36-37	22.66230113646877	26.690825725419376	28.576675560555543	22.070197577556314
38-39	22.626561776955114	26.769828782970844	28.486163813049515	22.117445627024527
40-41	22.65478883896115	26.713180244894634	28.481627352425914	22.1504035637183
42-43	22.646938392585632	26.690661512765978	28.397382750145812	22.26501734450258
44-45	22.790588061329167	26.768204208755762	28.082770348326758	22.35843738158831
46-47	22.780729287977433	26.886162901180484	27.986786600266928	22.346321210575155
48-49	22.679113034472557	26.97505178280811	27.875332889480692	22.470502293238646
50-51	22.840227164905407	27.085096210311573	27.71016036747373	22.36451625730929
52-53	22.83736608125683	27.066062571572875	27.668615977192886	22.42795536997741
54-55	22.78992265643442	27.0923725636314	27.63669352785711	22.481011252077074
56-57	22.883916236625705	27.249838300896357	27.47061887960796	22.395626582869983
58-59	22.81477658748986	27.090472055054747	27.58092246851101	22.513828888944378
60-61	22.757121523949188	27.074983728976125	27.818312609187135	22.349582137887552
62-63	22.798599043253567	27.10405057220547	27.721482540349722	22.37586784419124
64-65	22.781274943931134	27.04334201914583	27.672461529628993	22.502921507294047
66-67	22.799937113316254	27.12996273039184	27.600780534721775	22.469319621570133
68-69	22.812845351767507	27.04293343814302	27.627216609252535	22.517004600836938
70-71	22.781806074507045	26.93740520143539	27.662961044726426	22.617827679331135
72-73	22.721596385709343	27.147141369160494	27.463132792250438	22.66812945287972
74-75	22.645481893160955	27.31677625371406	27.41113088652653	22.62661096659846
76-77	22.61324799171249	27.15759680708878	27.392625411022575	22.836529790176154
78-79	22.720215547631252	27.1832802544824	27.339555988164125	22.756948209722218
80-81	22.810190754823047	27.259593077369754	27.19865377865328	22.73156238915392
82-83	22.780140214833057	27.220832341480268	27.28298332112235	22.716044122564323
84-85	22.903874690238617	27.146436945681025	27.3530525323832	22.596635831697164
86-87	22.83183055303549	27.22038103349318	27.257873396160043	22.68991501731129
88-89	22.73371891523616	27.130574521972246	27.339503384332836	22.79620317845876
90-91	22.87220557838814	27.332269937288007	27.10817957576419	22.68734490855966
92-93	22.991098065589206	27.213850866002897	27.164174686239882	22.630876382168008
94-95	22.82210756682459	27.286484126558346	27.225158493923864	22.666249812693202
96-97	22.871345272610412	27.163658727186128	27.291004850620315	22.67399114958314
98-99	22.977832699127404	27.088671053386143	27.100695375815576	22.83280087167088
100-101	22.82692567461294	27.256157699275196	27.27410666868978	22.642809957422084
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	224.0
1	160.0
2	108.5
3	125.0
4	118.0
5	116.5
6	152.5
7	244.0
8	437.5
9	572.5
10	471.0
11	359.5
12	383.0
13	426.0
14	460.5
15	483.5
16	519.5
17	549.0
18	574.5
19	630.0
20	690.0
21	809.5
22	1010.5
23	1260.5
24	1590.5
25	2049.5
26	2763.5
27	3719.0
28	4868.0
29	6247.5
30	7916.0
31	9682.5
32	10996.0
33	11860.5
34	12383.5
35	12907.5
36	13883.0
37	14450.5
38	14826.5
39	15105.0
40	15250.5
41	15643.5
42	15677.5
43	15976.5
44	16627.0
45	17175.0
46	17819.0
47	18440.5
48	19033.5
49	19405.5
50	19165.5
51	18845.0
52	18908.5
53	19149.5
54	18650.5
55	17662.0
56	16494.5
57	14872.5
58	13682.5
59	12384.0
60	10415.0
61	8490.5
62	6713.0
63	4983.0
64	3574.0
65	2617.5
66	1960.0
67	1484.0
68	1137.0
69	816.0
70	589.0
71	474.0
72	343.5
73	233.0
74	182.0
75	136.0
76	100.0
77	68.0
78	47.0
79	34.5
80	25.5
81	17.0
82	9.0
83	8.5
84	9.0
85	9.5
86	9.0
87	8.0
88	7.0
89	3.5
90	3.5
91	5.0
92	4.0
93	3.0
94	4.5
95	3.0
96	1.0
97	0.5
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.044882391511132866
2	0.06575362706980782
3	0.049684622701624444
4	0.059289085082607614
5	0.0927200022164144
6	0.07221816905700804
7	0.09881514180434602
8	0.1126677317769179
9	0.1124830305772836
10-11	0.0683394438646879
12-13	0.049222869702538724
14-15	0.1002004008016032
16-17	0.05393275029321315
18-19	0.029090438942400928
20-21	0.03416972193234395
22-23	0.08302318923561408
24-25	0.18193068163977724
26-27	0.18368534303630302
28-29	0.13861825032553587
30-31	0.155795461891525
32-33	0.21028231578364098
34-35	0.17869841064617714
36-37	0.1789754624456286
38-39	0.21517689757394975
40-41	0.15810422688695364
42-43	0.08597840842976275
44-45	0.07388047985371665
46-47	0.08293083863579695
48-49	0.12836733374583267
50-51	0.13861825032553587
52-53	0.16290645807744522
54-55	0.2387263005273219
56-57	0.19569092101253197
58-59	0.14582159711127324
60-61	0.2483307629083051
62-63	0.2503624761042823
64-65	0.26855554426826
66-67	0.14037291172206162
68-69	0.13898765272480446
70-71	0.14683745370926182
72-73	0.1651228724730567
74-75	0.16641578087049674
76-77	0.1556107606918907
78-79	0.1891340284255146
80-81	0.2832392896391862
82-83	0.2956142700146837
84-85	0.2366945873313447
86-87	0.24103506552275059
88-89	0.236232834332259
90-91	0.18617880923136595
92-93	0.16890924706555968
94-95	0.15847362928622222
96-97	0.14083466472114736
98-99	0.15588781249134212
100-101	0.18359299243648589
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	541415.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.58849457593095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.41587759172916	50.21829755955929
2	14.966725816669857	19.932234817255328
3	4.8532624885662745	9.695143286863935
4	2.0864001109365558	5.557209698812823
5	0.9919057824516513	3.3024756407308153
6	0.553820376442202	2.212683907965696
7	0.33502397949319374	1.5616119708902534
8	0.19522157816221594	1.0399608798847515
9	0.15666148322605156	0.9388667093449707
>10	0.43675507791678164	4.397641366704113
>50	0.0063983820848905045	0.3145423529299143
>100	0.0011127613263889557	0.10336545665739409
>500	2.781903315966136E-4	0.1394877936610794
>1k	5.563806631932272E-4	0.5864785587396779
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1734	0.32027188016586167	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	0.2644921178763056	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	753	0.1390800033246216	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.8470119963429163E-4	0.0
16-17	0.0	0.0	0.0	2.7705179945143745E-4	0.0
18-19	0.0	0.0	0.0	4.617529990857291E-4	0.0
20-21	0.0	0.0	0.0	5.541035989028749E-4	0.0
22-23	0.0	0.0	0.0	5.541035989028749E-4	0.0
24-25	0.0	0.0	0.0	8.311553983543123E-4	0.0
26-27	0.0	0.0	0.0	0.0012929083974400413	0.0
28-29	0.0	0.0	0.0	0.0016623107967086246	0.0
30-31	0.0	0.0	0.0	0.0031399203937829577	0.0
32-33	0.0	0.0	0.0	0.006926294986285936	0.0
34-35	0.0	0.0	0.0	0.01366788877293758	0.0
36-37	0.0	0.0	0.0	0.02114828735812639	0.0
38-39	0.0	0.0	0.0	0.03130685333801243	0.0
40-41	0.0	0.0	0.0	0.0407266145193613	0.0
42-43	0.0	0.0	0.0	0.04968462270162445	0.0
44-45	0.0	0.0	0.0	0.06187490187748769	0.0
46-47	0.0	0.0	0.0	0.07286462325572804	0.0
48-49	0.0	0.0	0.0	0.08440844823287127	0.0
50-51	0.0	0.0	0.0	0.0969681298080031	0.0
52-53	0.0	0.0	0.0	0.11359123777508934	0.0
54-55	0.0	0.0	0.0	0.1276285289472955	0.0
56-57	0.0	0.0	0.0	0.14295872851694172	0.0
58-59	0.0	0.0	0.0	0.15875068108567364	0.0
60-61	0.0	0.0	0.0	0.1731573746571484	0.0
62-63	0.0	0.0	0.0	0.18756406822862315	0.0
64-65	0.0	0.0	0.0	0.20751179778912665	0.0
66-67	0.0	0.0	0.0	0.2290294875465216	0.0
68-69	0.0	0.0	0.0	0.2515630339019052	0.0
70-71	0.0	0.0	0.0	0.27243426946058014	0.0
72-73	0.0	0.0	0.0	0.29256670022071796	0.0
74-75	0.0	0.0	0.0	0.3161161031740901	0.0
76-77	0.0	0.0	0.0	0.34021960972636517	0.0
78-79	0.0	0.0	0.0	0.3727270208620005	0.0
80-81	0.0	0.0	0.0	0.4094825595892245	0.0
82-83	0.0	0.0	0.0	0.4487315645115115	0.0
84-85	9.235059981714582E-5	0.0	0.0	0.48437889604092976	0.0
86-87	1.8470119963429163E-4	0.0	0.0	0.5237202515630339	0.0
88-89	1.8470119963429163E-4	0.0	0.0	0.5674020852765438	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	670	0.0	66.60693	1
GTATCAA	1410	0.0	47.138447	1
TCAACGC	1785	0.0	37.235413	4
TATCAAC	1815	0.0	36.61995	2
ATCAACG	1825	0.0	36.419296	3
CAACGCA	1840	0.0	36.1224	5
AACGCAG	1915	0.0	34.942657	6
ACGCAGA	2205	0.0	30.347027	7
GTACATG	1855	0.0	30.19983	1
GTCCGAT	65	0.0058563217	29.215477	1
TACATGG	1900	0.0	28.984827	2
CGCAGAG	2350	0.0	28.6765	8
ACATGGG	1980	0.0	27.573952	3
GTATAAG	205	9.604264E-10	25.474472	1
GCAGAGT	2590	0.0	25.471872	9
GTGGTAT	280	0.0	25.433119	1
GTCTAAG	190	1.1947122E-8	24.986921	1
GTTCGAA	95	0.0012314492	24.979986	9
TCTATAC	115	1.2850815E-4	24.769646	3
CATGGGG	1295	0.0	24.195831	4
>>END_MODULE