FastQCFastQC Report
Fri 10 Feb 2017
SRR2031401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541415
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.38103857484554365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.3178707645706159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.165307573672691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.065.387271
GTATCAA16300.041.116221
CGAATGC352.0530545E-433.9291356-57
TACCGTG702.0528169E-433.9267
GTGGTAT3200.031.1925581
TCAACGC21550.030.6385574
GTACCGT1102.7315436E-630.2249816
CAACGCA22000.030.0118625
ATCAACG22100.029.876063
AACGCAG22200.029.7387376
TATCAAC22300.029.6081122
TAACGAG700.00838704127.1433055
ACGCAGA25800.025.7732417
GTACATG19050.025.6994531
TACATGG18800.025.519042
CGCAGAG26850.025.1191398
ACATGGG19650.023.9316943
GAGTACT18550.023.30227312-13
GCAGAGT29250.022.89579
CAGAGTA28200.022.65342510-11
Produced by FastQC (version 0.11.5)