FastQCFastQC Report
Fri 10 Feb 2017
SRR2031400_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031400_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583357
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.11166148884932457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.051.3567161
GTATCAA30350.042.692061
TCAACGC35350.036.2634054
CAACGCA35700.035.907885
ATCAACG36200.035.14963
AACGCAG37400.034.2648666
TATCAAC40300.031.809212
ACGCAGA42950.029.9476667
CGCAGAG43450.029.712288
GTACATG54350.026.459781
TACATGG57650.024.871492
ACATGGG55900.024.6309073
GCAGAGT53450.023.9742769
CGTATAC1000.001661706423.730841
CATGGGG33600.022.7499684
GTATAGG6000.021.3577581
AGAGTAC48600.020.65396910-11
CGTTAGG1058.5926877E-720.36325352-53
CAGAGTA51550.019.74822410-11
GTATAGA7750.019.5970821
Produced by FastQC (version 0.11.5)