FastQCFastQC Report
Fri 10 Feb 2017
SRR2031400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583357
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26160.1652185830485481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.12100871755390603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.057.8506281
GTATCAA31500.044.2314761
TCAACGC36500.037.2168664
ATCAACG36950.036.763623
CAACGCA37300.036.5459865
AACGCAG38800.035.376846
TATCAAC42200.032.4150472
ACGCAGA44050.030.6214227
CGCAGAG44850.030.0752188
GTACATG53250.027.7725091
ACATGGG52200.026.569233
TACATGG55500.026.1019822
GCAGAGT55250.024.4140019
CATGGGG32900.022.5213974
CATGGGA25150.021.3406224
CAGAGTA52400.020.75664110-11
AGAGTAC50700.020.46898710-11
GTACTAT4200.019.2474841
GAGTACT30300.018.57616412-13
GTATATA9250.017.992881
Produced by FastQC (version 0.11.5)