FastQCFastQC Report
Fri 10 Feb 2017
SRR2031399_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031399_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17783
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.5679581622898273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.3542709329134567No Hit
CATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT590.33177754034752294Illumina Single End PCR Primer 1 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.26992071079120505No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC250.14058370353708596No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTACA156.5075513E-495.008487
GGTATCA451.9710933E-763.1605641
GAATCCT250.004938451657.0050856
CTCCAGA250.00499413756.844511
GTATCAA751.2916098E-744.2123951
ACATGGG550.002510791234.5485343
TCAACGC902.2810871E-531.6694934
TATCAAC902.2810871E-531.6694932
ATCAACG902.2810871E-531.6694933
CAACGCA1004.6840425E-528.5025425
AACGCAG1056.531831E-527.1452796
ACGCAGA1108.964954E-525.9114027
GAGTACT1051.1623342E-924.88317112-13
CGCAGAG1151.2127578E-424.7848198
CAGAGTA1202.0736479E-1023.7521210-11
GCAGAGT1201.6190055E-423.752129
GTACTTT1153.3323886E-922.71941814-15
AGTACTT1053.211062E-822.62106512-13
TACTTTT1105.2561518E-821.59283414-15
ACTTTTT1259.0149115E-920.84298516-17
Produced by FastQC (version 0.11.5)