FastQCFastQC Report
Fri 10 Feb 2017
SRR2031399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17783
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT3491.9625485013777204No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG1610.9053590507788337RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1340.7535286509587809RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.6579317325535624No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT1130.6354383399876287RNA PCR Primer, Index 17 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.4386211550357083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.32053084406455606No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA550.3092841477815892RNA PCR Primer, Index 17 (96% over 28bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT490.2755440589326885RNA PCR Primer, Index 17 (96% over 26bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG360.2024405330934038TruSeq Adapter, Index 12 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA452.1482265E-974.287691
CAACTCT200.002042654471.230994
AGCTACA200.002042654471.230997
CAACGCA505.173206E-966.482255
AACGCAG551.0990334E-860.438416
ACGCAGA551.0990334E-860.438417
TCAACGC551.0990334E-860.438414
TATCAAC551.0990334E-860.438412
CCAGTGT250.004945649356.9847873
ATACTAT250.004945649356.9847876
CAGTGTC250.004945649356.9847874
ATCAACG602.1844244E-855.401883
CGCAGAG654.1063686E-851.1401948
GCAGAGT652.4521105E-643.834459
GAGTACT352.0168233E-433.91951812-13
AGAGTAC552.6419075E-630.21920610-11
CAGAGTA652.906072E-729.22296710-11
AGTACTT350.00829518327.13561412-13
TATACAC1801.7408274E-723.7436623
CTTATAC1902.7767237E-722.6214391
Produced by FastQC (version 0.11.5)