##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031399_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17783 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.133948152730134 34.0 33.0 34.0 31.0 34.0 2 33.26002361806219 34.0 34.0 34.0 31.0 34.0 3 33.341730866557945 34.0 34.0 34.0 31.0 34.0 4 36.569813867176514 37.0 37.0 37.0 35.0 37.0 5 36.467525164482936 37.0 37.0 37.0 35.0 37.0 6 36.505595231400775 37.0 37.0 37.0 35.0 37.0 7 36.51481752235281 37.0 37.0 37.0 35.0 37.0 8 36.48169600179947 37.0 37.0 37.0 35.0 37.0 9 38.39661474441883 39.0 39.0 39.0 37.0 39.0 10-11 38.458724624641505 39.0 39.0 39.0 37.0 39.0 12-13 38.466709779002414 39.0 39.0 39.0 37.0 39.0 14-15 40.10048923128831 41.0 40.0 41.0 38.0 41.0 16-17 40.14795028960243 41.0 40.0 41.0 38.0 41.0 18-19 40.207670246864986 41.0 40.0 41.0 38.5 41.0 20-21 40.18062194230445 41.0 40.0 41.0 39.0 41.0 22-23 40.155766743519095 41.0 40.0 41.0 38.0 41.0 24-25 40.114378901197774 41.0 40.0 41.0 38.0 41.0 26-27 39.99609177304167 41.0 40.0 41.0 38.0 41.0 28-29 39.8639149749761 41.0 40.0 41.0 38.0 41.0 30-31 39.644801214643195 41.0 40.0 41.0 37.0 41.0 32-33 39.426193555643025 41.0 40.0 41.0 35.5 41.0 34-35 39.339762694708426 41.0 40.0 41.0 35.0 41.0 36-37 39.2308665579486 41.0 39.5 41.0 35.0 41.0 38-39 38.944441320362145 41.0 39.0 41.0 35.0 41.0 40-41 38.864786593938035 41.0 39.0 41.0 35.0 41.0 42-43 38.59548445144239 41.0 39.0 41.0 35.0 41.0 44-45 38.35190912669403 41.0 38.0 41.0 35.0 41.0 46-47 38.07990777709048 40.0 38.0 41.0 33.5 41.0 48-49 37.74385649215543 40.0 38.0 41.0 33.0 41.0 50-51 37.4909182927515 40.0 38.0 41.0 32.5 41.0 52-53 37.52603610189507 40.0 38.0 41.0 33.0 41.0 54-55 37.449839734577964 40.0 37.5 41.0 33.0 41.0 56-57 37.2710172636788 40.0 37.0 41.0 32.5 41.0 58-59 37.003092841477816 40.0 36.5 41.0 31.0 41.0 60-61 36.62084575156048 40.0 35.5 41.0 30.5 41.0 62-63 36.27815891581848 39.0 35.0 41.0 29.0 41.0 64-65 35.861243884608896 39.0 35.0 41.0 27.5 41.0 66-67 35.332621042568746 38.5 35.0 41.0 25.0 41.0 68-69 34.76845864027442 37.0 35.0 40.5 21.5 41.0 70-71 34.225693077658434 37.0 35.0 39.5 19.5 41.0 72-73 33.76615306753641 36.0 35.0 39.0 18.0 41.0 74-75 33.360203565202724 36.0 35.0 39.0 17.0 41.0 76-77 32.300596074903 35.0 33.0 37.0 16.0 39.0 78-79 32.679609739638984 35.0 34.0 37.0 17.0 39.0 80-81 32.49004667378958 35.0 34.0 36.5 17.0 39.0 82-83 32.2024124163527 35.0 34.0 36.0 16.0 37.5 84-85 31.962070516785694 35.0 34.0 36.0 15.0 37.0 86-87 31.803014114603833 35.0 34.0 35.5 15.0 37.0 88-89 31.634173086655792 35.0 34.0 35.0 13.5 36.0 90-91 31.465219591744926 35.0 34.0 35.0 11.0 36.0 92-93 31.336669853230614 35.0 34.0 35.0 10.0 36.0 94-95 31.278468199966262 35.0 34.0 35.0 10.0 36.0 96-97 31.19279649103076 35.0 34.0 35.0 10.0 35.5 98-99 31.120761401338356 35.0 34.0 35.0 10.0 35.0 100-101 30.87448686948209 35.0 33.5 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38035372893222075 1101 2 -0.12189651570875526 1101 3 0.10514935612756915 1101 4 0.2508823217111811 1101 5 0.2918822882374812 1101 6 0.1394121355614999 1101 7 -0.1990276382801781 1101 8 0.0669301217956857 1101 9 0.2830915121424056 1101 10-11 0.016586902961186922 1101 12-13 0.06759356953378415 1101 14-15 0.060097047097237066 1101 16-17 -3.165307560877295E-4 1101 18-19 -0.08869409917541304 1101 20-21 0.16972136421798467 1101 22-23 0.061780282384042096 1101 24-25 0.25288687736824045 1101 26-27 -0.341279087981853 1101 28-29 0.4343566553573268 1101 30-31 0.30202239322294133 1101 32-33 -0.09584708460584324 1101 34-35 0.22641184855013563 1101 36-37 0.8636073724500619 1101 38-39 1.3789291471699414 1101 40-41 0.9333351910961696 1101 42-43 0.5687858166032882 1101 44-45 0.9466103918339783 1101 46-47 0.30925218366521534 1101 48-49 1.0403596409018476 1101 50-51 -0.31919205896149805 1101 52-53 0.4467587377497537 1101 54-55 0.013672826378055447 1101 56-57 1.1039154681226577 1101 58-59 1.4888723500398342 1101 60-61 1.0554001934483495 1101 62-63 1.210652585787642 1101 64-65 1.3956664556881293 1101 66-67 0.8520385929650587 1101 68-69 1.1893711966537381 1101 70-71 1.6321009636214754 1101 72-73 2.242047643095134 1101 74-75 2.3906004423088874 1101 76-77 2.140104213865534 1101 78-79 2.572959006634413 1101 80-81 2.579822761123843 1101 82-83 2.8877444137745982 1101 84-85 2.869426056312193 1101 86-87 1.8232485102689466 1101 88-89 1.6291086583675316 1101 90-91 1.560759758636106 1101 92-93 1.4187917022473506 1101 94-95 1.308758533678656 1101 96-97 1.5918911693712552 1101 98-99 1.1758671979196116 1101 100-101 1.1885692324726307 1102 1 0.5053537289322207 1102 2 0.5031034842912447 1102 3 0.6051493561275691 1102 4 0.3758823217111811 1102 5 0.1668822882374812 1102 6 0.0769121355614999 1102 7 0.11347236171982189 1102 8 0.3169301217956857 1102 9 0.3455915121424056 1102 10-11 0.4853369029611869 1102 12-13 0.28634356953378415 1102 14-15 0.43509704709723707 1102 16-17 0.34343346924391227 1102 18-19 0.505055900824587 1102 20-21 0.26347136421798467 1102 22-23 0.1867802823840421 1102 24-25 0.002886877368240448 1102 26-27 0.283720912018147 1102 28-29 0.2781066553573268 1102 30-31 0.14577239322294133 1102 32-33 0.6854029153941568 1102 34-35 0.5076618485501356 1102 36-37 0.7073573724500619 1102 38-39 0.5664291471699414 1102 40-41 1.0270851910961696 1102 42-43 1.1937858166032882 1102 44-45 1.2591103918339783 1102 46-47 0.8717521836652153 1102 48-49 1.1028596409018476 1102 50-51 1.212057941038502 1102 52-53 2.0092587377497537 1102 54-55 1.1074228263780554 1102 56-57 0.8851654681226577 1102 58-59 -0.6361276499601658 1102 60-61 -0.41334980655165054 1102 62-63 0.366902585787642 1102 64-65 0.8331664556881293 1102 66-67 1.4145385929650587 1102 68-69 1.6581211966537381 1102 70-71 2.4133509636214754 1102 72-73 2.054547643095134 1102 74-75 1.8593504423088874 1102 76-77 1.4213542138655342 1102 78-79 1.479209006634413 1102 80-81 1.5798227611238431 1102 82-83 1.6689944137745982 1102 84-85 1.869426056312193 1102 86-87 1.5107485102689466 1102 88-89 1.4728586583675316 1102 90-91 1.217009758636106 1102 92-93 1.5437917022473506 1102 94-95 1.152508533678656 1102 96-97 1.5606411693712552 1102 98-99 0.6133671979196116 1102 100-101 1.6260692324726307 1103 1 -0.24464627106777925 1103 2 -0.43439651570875526 1103 3 0.04264935612756915 1103 4 8.823217111810777E-4 1103 5 -1.1456177117625188 1103 6 -0.2980878644385001 1103 7 -0.07402763828017811 1103 8 -0.1205698782043143 1103 9 0.09559151214240558 1103 10-11 0.11033690296118692 1103 12-13 0.25509356953378415 1103 14-15 -0.9711529529027629 1103 16-17 -0.6878165307560877 1103 18-19 -0.43244409917541304 1103 20-21 -0.08027863578201533 1103 22-23 -0.031969717615957904 1103 24-25 -0.6846131226317596 1103 26-27 -0.18502908798185302 1103 28-29 -0.003143344642673185 1103 30-31 0.14577239322294133 1103 32-33 -0.12709708460584324 1103 34-35 -0.21108815144986437 1103 36-37 0.17610737245006192 1103 38-39 -0.15232085283005858 1103 40-41 0.4020851910961696 1103 42-43 0.6000358166032882 1103 44-45 1.4153603918339783 1103 46-47 -0.25324781633478466 1103 48-49 -1.7408903590981524 1103 50-51 -1.037942058961498 1103 52-53 -0.8657412622502463 1103 54-55 -1.9238271736219446 1103 56-57 -1.3960845318773423 1103 58-59 -2.479877649960166 1103 60-61 -3.8508498065516505 1103 62-63 -3.070597414212358 1103 64-65 -1.4480835443118707 1103 66-67 -1.9292114070349413 1103 68-69 -3.591878803346262 1103 70-71 -3.2428990363785246 1103 72-73 -3.570452356904866 1103 74-75 -3.1718995576911126 1103 76-77 -3.672395786134466 1103 78-79 -3.083290993365587 1103 80-81 -2.982677238876157 1103 82-83 -2.9872555862254018 1103 84-85 -3.505573943687807 1103 86-87 -1.7392514897310534 1103 88-89 -2.6521413416324684 1103 90-91 -2.126740241363894 1103 92-93 -2.6437082977526494 1103 94-95 -1.784991466321344 1103 96-97 -3.751858830628745 1103 98-99 -2.9491328020803884 1103 100-101 -2.4676807675273693 1104 1 0.5400759511544422 1104 2 0.19060348429124474 1104 3 -0.06151731053909515 1104 4 0.042548988377845376 1104 5 0.31966006601525976 1104 6 0.3408010244503856 1104 7 0.4259723617198219 1104 8 0.4419301217956857 1104 9 0.3664248454757413 1104 10-11 0.37422579185007265 1104 12-13 0.137038013978227 1104 14-15 0.1156526026527942 1104 16-17 0.2219056914661337 1104 18-19 0.18908367860236552 1104 20-21 0.3155546975513204 1104 22-23 0.1659469490507135 1104 24-25 0.5896924329237976 1104 26-27 0.6830264675737041 1104 28-29 0.6079677664684411 1104 30-31 0.43049461544516276 1104 32-33 0.0222084709497139 1104 34-35 7.174041056927649E-4 1104 36-37 0.06499626133894765 1104 38-39 -0.23218196394117285 1104 40-41 0.6694463022072838 1104 42-43 0.5097580388255096 1104 44-45 1.3007770585006497 1104 46-47 -0.27408114966811326 1104 48-49 -1.8276959146537095 1104 50-51 -2.301830947850384 1104 52-53 -1.6504634844724677 1104 54-55 -1.9134105069552803 1104 56-57 -1.878723420766228 1104 58-59 -2.448627649960166 1104 60-61 -1.4584886954405363 1104 62-63 -2.1122640808790294 1104 64-65 -2.6182224332007564 1104 66-67 -2.29379474036827 1104 68-69 -1.4842399144573761 1104 70-71 -0.652621258600746 1104 72-73 -0.36211902357153036 1104 74-75 -1.300371779913334 1104 76-77 -1.3876735639122444 1104 78-79 -1.6839854378100299 1104 80-81 -3.1736494610983783 1104 82-83 -2.9837833640031803 1104 84-85 -2.2764072770211428 1104 86-87 -2.204529267508832 1104 88-89 -4.214641341632468 1104 90-91 -3.126740241363894 1104 92-93 -2.4562082977526494 1104 94-95 -2.0488803552102333 1104 96-97 -2.387275497295409 1104 98-99 -2.563716135413724 1104 100-101 -2.637819656416255 1105 1 -0.2409698004795473 1105 2 -0.07410239806169727 1105 3 -0.15955652622537286 1105 4 0.1405882040641231 1105 5 0.1889411117668942 1105 6 0.09897095909091291 1105 7 0.3083253028962929 1105 8 0.030165415913330662 1105 9 0.1801503356718186 1105 10-11 0.10298396178471592 1105 12-13 0.3543582754161321 1105 14-15 0.045391164744295054 1105 16-17 0.014389351596854283 1105 18-19 -0.07398821682247103 1105 20-21 0.055750775982694734 1105 22-23 0.2786920470899261 1105 24-25 0.28413687736824045 1105 26-27 0.08335326495932094 1105 28-29 0.17332724359261675 1105 30-31 0.7376841579288254 1105 32-33 -0.12158237872349531 1105 34-35 0.47457361325601966 1105 36-37 0.7055191371559459 1105 38-39 0.9377526765817095 1105 40-41 0.5289234263902856 1105 42-43 1.0228299342503462 1105 44-45 1.5752868624222103 1105 46-47 1.8680757130769834 1105 48-49 1.7425655232547896 1105 50-51 1.587057941038502 1105 52-53 1.6011705024556377 1105 54-55 1.4640404734368815 1105 56-57 1.1425184092991287 1105 58-59 -0.06627470878369479 1105 60-61 0.13628254638952342 1105 62-63 0.1867555269641059 1105 64-65 0.6824311615704772 1105 66-67 0.8189503576709427 1105 68-69 0.7298123731243251 1105 70-71 1.3490127283273594 1105 72-73 1.2457241136833659 1105 74-75 0.43655632466182936 1105 76-77 0.9048100962184762 1105 78-79 0.1134001831050071 1105 80-81 1.0485727611238431 1105 82-83 0.7939944137745982 1105 84-85 0.9686907621945409 1105 86-87 1.0291308632101206 1105 88-89 1.0059468936616476 1105 90-91 1.358553876283164 1105 92-93 1.2937917022473506 1105 94-95 0.692949710149243 1105 96-97 0.9411558752536102 1105 98-99 0.9902054332137276 1105 100-101 1.0745986442373407 1106 1 0.06785372893222075 1106 2 -0.30939651570875526 1106 3 -0.45735064387243085 1106 4 -0.12411767828881892 1106 5 0.1668822882374812 1106 6 -0.2355878644385001 1106 7 -0.1990276382801781 1106 8 -0.1830698782043143 1106 9 -0.029408487857594423 1106 10-11 0.11033690296118692 1106 12-13 0.06759356953378415 1106 14-15 0.18509704709723707 1106 16-17 -0.12531653075608773 1106 18-19 0.16130590082458696 1106 20-21 -0.08027863578201533 1106 22-23 0.061780282384042096 1106 24-25 0.09663687736824045 1106 26-27 -0.060029087981853024 1106 28-29 -0.12814334464267318 1106 30-31 0.45827239322294133 1106 32-33 0.21665291539415676 1106 34-35 0.00766184855013563 1106 36-37 0.8636073724500619 1106 38-39 1.0976791471699414 1106 40-41 0.6833351910961696 1106 42-43 1.2875358166032882 1106 44-45 1.1653603918339783 1106 46-47 1.5592521836652153 1106 48-49 1.6341096409018476 1106 50-51 1.524557941038502 1106 52-53 1.5717587377497537 1106 54-55 1.2011728263780554 1106 56-57 1.3851654681226577 1106 58-59 2.145122350039834 1106 60-61 2.3366501934483495 1106 62-63 2.554402585787642 1106 64-65 2.0831664556881293 1106 66-67 1.3207885929650587 1106 68-69 2.345621196653738 1106 70-71 1.7571009636214754 1106 72-73 2.710797643095134 1106 74-75 2.3593504423088874 1106 76-77 0.9526042138655342 1106 78-79 1.729209006634413 1106 80-81 2.267322761123843 1106 82-83 2.2627444137745982 1106 84-85 3.056926056312193 1106 86-87 3.2919985102689466 1106 88-89 2.2541086583675316 1106 90-91 2.154509758636106 1106 92-93 2.7625417022473506 1106 94-95 2.277508533678656 1106 96-97 2.654391169371255 1106 98-99 2.1446171979196116 1106 100-101 2.6885692324726307 1107 1 0.053147846579278735 1107 2 -0.30939651570875526 1107 3 0.07573759142168512 1107 4 -0.0947059135829349 1107 5 0.012470523531597166 1107 6 -0.07749962914438413 1107 7 -0.3387335206331201 1107 8 0.2066360041486277 1107 9 0.1801503356718186 1107 10-11 0.2206310206082449 1107 12-13 0.1484759224749581 1107 14-15 0.16303822356783115 1107 16-17 -0.2209047660502037 1107 18-19 -0.956341157998942 1107 20-21 -0.3854256946055443 1107 22-23 -0.30954324702772595 1107 24-25 -0.6864513579258755 1107 26-27 -0.093117323275969 1107 28-29 0.40862136123967474 1107 30-31 0.5023900402817674 1107 32-33 0.31959409186475085 1107 34-35 0.47457361325601966 1107 36-37 0.35257796068535185 1107 38-39 -0.3857767351830006 1107 40-41 -0.3240177500803014 1107 42-43 0.1698887577797592 1107 44-45 0.4576398035986884 1107 46-47 1.1916051248416863 1107 48-49 0.6249184644312606 1107 50-51 -0.20705970602032409 1107 52-53 0.9835234436321088 1107 54-55 0.9934522381427584 1107 56-57 0.6719301740050057 1107 58-59 -0.36039235584252083 1107 60-61 -0.30489392419870853 1107 62-63 -0.342656237741771 1107 64-65 -0.2587453090177547 1107 66-67 0.20130329884741371 1107 68-69 0.22981237312433223 1107 70-71 -0.033340212849111595 1107 72-73 -1.666040592198982 1107 74-75 -0.5928554400440547 1107 76-77 -1.3304840214285818 1107 78-79 -0.504246875718529 1107 80-81 -1.2749566506408598 1107 82-83 -0.6471820568136337 1107 84-85 -0.531309237805452 1107 86-87 -0.41204560737811136 1107 88-89 0.4177115995440026 1107 90-91 -0.14144612371683607 1107 92-93 -0.5297377095173559 1107 94-95 0.07530265132571401 1107 96-97 -1.2353147129816868 1107 98-99 -0.39214750796274345 1107 100-101 -0.6312837087038403 1108 1 -0.1321462710677821 1108 2 0.04060348429124616 1108 3 0.25514935612756773 1108 4 0.27588232171117966 1108 5 -1.4081177117625217 1108 6 0.0019121355614970525 1108 7 0.07597236171982047 1108 8 -0.20806987820431289 1108 9 0.03309151214240558 1108 10-11 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-0.5488200890100998 2312 72-73 0.751916064147764 2312 74-75 0.6981662317825723 2312 76-77 0.9032621086023767 2312 78-79 1.1288800592659882 2312 80-81 0.23278328743963073 2312 82-83 1.1887312558798584 2312 84-85 0.37271552999640534 2312 86-87 -0.3083304370994746 2312 88-89 -0.09622028900088964 2312 90-91 0.36010186389926346 2312 92-93 1.5043180180368232 2312 94-95 1.313692744204971 2312 96-97 0.4767595904238888 2312 98-99 0.5196171979196116 2312 100-101 -0.11735182015894807 2313 1 -0.6821462710677793 2313 2 -0.7593965157087581 2313 3 -0.14485064387243085 2313 4 0.17588232171117824 2313 5 -1.1581177117625217 2313 6 -0.09808786443849726 2313 7 0.22597236171981905 2313 8 0.24193012179568285 2313 9 0.2830915121424056 2313 10-11 -2.1896630970388102 2313 12-13 -1.1574064304662102 2313 14-15 -0.3899029529027658 2313 16-17 -0.025316530756093414 2313 18-19 0.2613059008245884 2313 20-21 -0.01777863578201533 2313 22-23 0.22428028238404352 2313 24-25 0.05913687736823903 2313 26-27 0.15247091201815266 2313 28-29 0.5718566553573297 2313 30-31 0.7082723932229413 2313 32-33 0.5416529153941596 2313 34-35 0.6451618485501385 2313 36-37 0.8011073724500619 2313 38-39 1.0539291471699386 2313 40-41 0.908335191096171 2313 42-43 1.1625358166032882 2313 44-45 0.18411039183397548 2313 46-47 0.6092521836652125 2313 48-49 0.2778596409018448 2313 50-51 0.5120579410384991 2313 52-53 0.5717587377497537 2313 54-55 0.19492282637805403 2313 56-57 0.010165468122657728 2313 58-59 0.5263723500398356 2313 60-61 -0.21959980655164912 2313 62-63 0.5044025857876449 2313 64-65 0.2706664556881293 2313 66-67 1.5395385929650587 2313 68-69 1.0518711966537353 2313 70-71 0.4446009636214754 2313 72-73 0.8795476430951368 2313 74-75 1.6218504423088902 2313 76-77 0.7901042138655399 2313 78-79 0.4104590066344116 2313 80-81 0.8985727611238445 2313 82-83 0.8189944137746039 2313 84-85 0.2569260563121958 2313 86-87 0.5732485102689466 2313 88-89 -0.6896413416324698 2313 90-91 -0.27674024136389264 2313 92-93 0.3437917022473478 2313 94-95 0.340008533678656 2313 96-97 0.30439116937125377 2313 98-99 0.41961719791961016 2313 100-101 0.5010692324726307 2314 1 -0.225624531937342 2314 2 -0.30939651570875526 2314 3 -0.4383289047419936 2314 4 -1.1458568087236003 2314 5 -0.19724814654512812 2314 6 0.01712952686585112 2314 7 0.0781462747632986 2314 8 -1.4711133564651817 2314 9 -1.4669084878575944 2314 10-11 -0.8842283144301177 2314 12-13 -0.8400151261183879 2314 14-15 -0.6899029529027629 2314 16-17 -0.46770783510391567 2314 18-19 -0.5126071426536782 2314 20-21 -0.9851699401298433 2314 22-23 -0.20724145674639516 2314 24-25 -1.0636892095882757 2314 26-27 -0.731224740155767 2314 28-29 -0.6118389968165872 2314 30-31 -0.7917276067770587 2314 32-33 -1.8159557802580153 2314 34-35 -1.4461424992759504 2314 36-37 -0.45432741015864053 2314 38-39 -0.22976650500397255 2314 40-41 -0.02862133064295591 2314 42-43 -2.0168120094836652 2314 44-45 -2.4745852603399356 2314 46-47 -2.6690086859000033 2314 48-49 -4.761270793880762 2314 50-51 -4.347724667657154 2314 52-53 -4.3847630013806835 2314 54-55 -5.0474684779697725 2314 56-57 -3.95722583622517 2314 58-59 -3.3181928673514705 2314 60-61 -3.982643284812525 2314 62-63 -5.912988718560186 2314 64-65 -4.924985718224917 2314 66-67 -3.2756787983392925 2314 68-69 -4.1883461946506095 2314 70-71 -3.1803990363785246 2314 72-73 -3.978061052557038 2314 74-75 -3.458584340299808 2314 76-77 -3.164243612221423 2314 78-79 -2.8917149064090637 2314 80-81 -2.2122968040935476 2314 82-83 -2.162527325355839 2314 84-85 -2.725682639339979 2314 86-87 -2.220229750600616 2314 88-89 -3.4863804720672498 2314 90-91 -2.702827197885636 2314 92-93 -2.3149039499265633 2314 94-95 -1.5947740750169963 2314 96-97 -2.361913178454831 2314 98-99 -2.65429584555865 2314 100-101 -2.4880612023099786 2315 1 0.1814900925685876 2315 2 -0.03666924298148189 2315 3 -0.16757791659970422 2315 4 0.1031550489839077 2315 5 0.2691550155102078 2315 6 0.31554849919786676 2315 7 0.38051781626527514 2315 8 0.2601119399775058 2315 9 0.3512733303242257 2315 10-11 0.23533690296118692 2315 12-13 -0.1028609759207626 2315 14-15 -0.3717211347209428 2315 16-17 -0.2957710762106345 2315 18-19 0.047669537188227196 2315 20-21 -0.24505136305474196 2315 22-23 0.13564391874768233 2315 24-25 0.05686415009551382 2315 26-27 0.2638345483817872 2315 28-29 0.11049301899369368 2315 30-31 0.026454211404761452 2315 32-33 -0.010619811878569863 2315 34-35 0.5360709394592291 2315 36-37 0.2670164633591554 2315 38-39 0.8107473289881284 2315 40-41 0.8537897365507163 2315 42-43 0.2875358166032882 2315 44-45 0.13411039183397833 2315 46-47 -0.05438417997114442 2315 48-49 -0.04486763182542575 2315 50-51 -0.07203296805240456 2315 52-53 0.4808496468406602 2315 54-55 0.5108319172871489 2315 56-57 0.39652910448629797 2315 58-59 0.27864507731256083 2315 60-61 0.521309284357443 2315 62-63 0.8271298585149154 2315 64-65 0.9752119102335826 2315 66-67 1.2895385929650587 2315 68-69 1.740507560290098 2315 70-71 1.9559645999851156 2315 72-73 2.0318203703678606 2315 74-75 2.330941351399794 2315 76-77 1.7082860320473472 2315 78-79 1.828640824816226 2315 80-81 2.048572761123843 2315 82-83 2.021267141047325 2315 84-85 1.6478351472212864 2315 86-87 1.8687030557234934 2315 88-89 1.9671768401857186 2315 90-91 2.1005324859088397 2315 92-93 1.9983371567928039 2315 94-95 1.771826715496836 2315 96-97 1.824845714825802 2315 98-99 1.9968899251923382 2315 100-101 1.4101601415635372 2316 1 -0.43214627106777925 2316 2 -0.5593965157087553 2316 3 -0.26985064387243085 2316 4 -0.16578434495548322 2316 5 -0.5414510450958545 2316 6 -0.048087864438500105 2316 7 0.009305695053157592 2316 8 -0.7247365448709786 2316 9 -0.7585751545242587 2316 10-11 -0.5563297637054774 2316 12-13 -0.45323976379955155 2316 14-15 -0.7315696195694343 2316 16-17 -0.8753165307560877 2316 18-19 -1.3178607658420844 2316 20-21 -1.101111969115344 2316 22-23 -0.9590530509492865 2316 24-25 -1.695029789298431 2316 26-27 -0.7683624213151816 2316 28-29 -0.6698100113093446 2316 30-31 -1.0000609401103873 2316 32-33 -2.6583470846058432 2316 34-35 -0.5131714847832001 2316 36-37 -0.8030592942166095 2316 38-39 -1.3919041861633872 2316 40-41 -0.4833314755704947 2316 42-43 0.1208691499366239 2316 44-45 -0.6992229414993574 2316 46-47 1.017585516998544 2316 48-49 -0.20964035909815237 2316 50-51 -0.39210872562816235 2316 52-53 0.030092071083082317 2316 54-55 -0.03841050695527315 2316 56-57 -0.48983453187734227 2316 58-59 -0.8652943166268301 2316 60-61 -2.3820998065516505 2316 62-63 -1.7997640808790294 2316 64-65 -1.333500210978542 2316 66-67 -0.5021280737016056 2316 68-69 -1.2689621366795905 2316 70-71 -2.138732369711853 2316 72-73 -1.3412856902381947 2316 74-75 -1.2239828910244483 2316 76-77 -0.2765624528011372 2316 78-79 -0.4687076600322584 2316 80-81 -0.13892723887615688 2316 82-83 0.06482774710793393 2316 84-85 0.41109272297886434 2316 86-87 -0.30175148973105337 2316 88-89 -0.11047467496580055 2316 90-91 -0.29340690803055836 2316 92-93 -0.4145416310859851 2316 94-95 -0.05582479965467968 2316 96-97 0.6127245027045838 2316 98-99 0.6237838645862759 2316 100-101 0.35523589913929854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 3.0 14 13.0 15 7.0 16 16.0 17 25.0 18 47.0 19 81.0 20 94.0 21 110.0 22 167.0 23 191.0 24 180.0 25 225.0 26 257.0 27 239.0 28 313.0 29 331.0 30 235.0 31 162.0 32 178.0 33 268.0 34 305.0 35 508.0 36 1169.0 37 3455.0 38 6680.0 39 2518.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.93729948781449 18.613159227781843 14.037260088928912 25.412281195474755 2 14.356407805207219 20.705167856942023 41.14041500309284 23.798009334757914 3 16.572006972951694 27.188888264072432 32.35674520609571 23.882359556880168 4 11.67407074171962 19.912275768992856 37.091604341224766 31.32204914806276 5 12.298262385424282 37.19844795591295 35.83759770567396 14.66569195298881 6 27.869313389191923 39.87516167125907 18.15216780070854 14.103357138840467 7 24.517797896867798 34.206826744643756 21.458696507900804 19.81667885058764 8 25.057639318450203 35.59017038744869 20.86262160490356 18.489568689197547 9 25.468143732778497 15.256143507844572 18.635775740876117 40.63993701850082 10-11 23.03885733565765 26.395996176123266 28.83371759545634 21.73142889276275 12-13 25.746499465781923 23.96952145307316 28.139234099983128 22.144744981161786 14-15 22.752066580441994 24.824270370578645 25.78305122870157 26.640611820277794 16-17 21.61615025586234 29.18517685429905 25.709947702862284 23.488725186976325 18-19 22.135365407867727 26.80876191547395 28.633692320670363 22.422180355987965 20-21 23.46407985378884 24.91494446787572 28.845775340925066 22.775200337410375 22-23 21.79328572231907 26.786818871956363 29.047404824832707 22.372490580891863 24-25 22.69583309902716 27.03705786425238 28.878704380588204 21.388404656132263 26-27 22.552437721419334 27.768093122645222 28.82528257324411 20.854186582691334 28-29 21.48400157453748 26.640611820277794 29.415734128099867 22.45965247708486 30-31 23.578698757240062 25.99111510993646 29.3904290614632 21.039757071360288 32-33 21.444638137547095 27.54597087105663 29.502896024292863 21.50649496710341 34-35 22.12787493673733 27.888995107687116 29.47759095765619 20.505538997919363 36-37 21.656122592436382 27.75481512723183 28.513988471812173 22.075073808519612 38-39 21.579598492942697 25.867401450823817 30.107405949502336 22.44559410673115 40-41 21.15784738233144 25.676207614013386 30.425125119496148 22.740819884159027 42-43 22.341562166113704 24.86925715571051 31.220828881516056 21.568351796659734 44-45 21.32092447843446 26.37631445762807 30.501040319406176 21.801720744531295 46-47 22.671279109011138 25.030937113286083 28.737765777927777 23.560017999775003 48-49 21.846707529663163 25.83366136197492 29.21329359500647 23.106337513355452 50-51 21.883259292582803 26.528144857448126 29.266715402350556 22.32188044761851 52-53 21.06506213799696 27.486925715571054 29.823426868357423 21.624585278074566 54-55 21.32092447843446 27.166394871506494 28.94337288421526 22.56930776584378 56-57 22.060394759039532 28.350109655288758 26.79806556823933 22.79143001743238 58-59 20.27779339818928 29.24984535792611 28.620030366079963 21.852330877804647 60-61 21.708935500196816 30.208626216049034 26.76432547939043 21.318112804363718 62-63 20.636036441345183 31.157912495782252 26.29906647171297 21.9069845911596 64-65 20.5601169722191 30.952648745922843 26.81363176245642 21.67360251940164 66-67 20.74511457893997 30.64248558976522 26.26739772247997 22.345002108814846 68-69 21.557711232953746 30.234781386194292 26.107127794179668 22.10037958667229 70-71 21.478378226395996 29.92464713490412 26.45785300567958 22.139121633020302 72-73 21.385592982061517 29.35950064668504 26.249789124444696 23.00511724680875 74-75 21.563941063997298 30.058486109548983 26.36936227645934 22.008210549994374 76-77 21.428973118884265 29.482060510628727 26.03756607805646 23.05140029243055 78-79 21.441826463476353 29.387617387392456 26.415677894618455 22.754878254512736 80-81 21.618961929933082 29.393240735533936 26.061406961704996 22.92639037282798 82-83 22.276893662486643 28.591913625372545 26.07827700612945 23.05291570601136 84-85 21.855142551875385 28.583478603160323 26.092335376483156 23.469043468481136 86-87 22.147556655232524 28.600348647584774 25.816791317550468 23.435303379632234 88-89 21.89169431479503 28.094247314851263 26.30321093178879 23.710847438564922 90-91 22.288140358769613 27.95928695945566 26.497216442669963 23.255356239104763 92-93 22.4287240623067 27.675307878310747 26.452229657538094 23.443738401844456 94-95 22.273775378212697 27.731848602440806 26.140262077498456 23.85411394184804 96-97 22.077264803463983 27.692177922735194 26.398807850194007 23.831749423606816 98-99 22.45402912894337 27.444750604509927 25.98268008772423 24.11854017882247 100-101 22.397795647528536 27.953663611314177 26.112017094978352 23.536523646178935 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 3.5 3 3.5 4 2.5 5 4.5 6 3.0 7 7.5 8 18.5 9 20.0 10 15.5 11 13.0 12 17.0 13 23.5 14 26.0 15 23.5 16 24.0 17 28.5 18 32.0 19 30.5 20 36.0 21 42.5 22 47.0 23 59.0 24 56.5 25 57.5 26 80.5 27 108.5 28 123.0 29 144.0 30 189.5 31 206.5 32 243.0 33 298.0 34 326.0 35 357.5 36 422.5 37 486.0 38 527.0 39 572.0 40 611.5 41 655.0 42 668.0 43 677.0 44 709.0 45 706.0 46 683.5 47 686.0 48 715.0 49 706.0 50 691.5 51 682.0 52 656.0 53 616.5 54 567.0 55 532.0 56 489.5 57 441.5 58 391.5 59 318.0 60 238.5 61 189.0 62 144.0 63 101.5 64 70.0 65 49.0 66 30.5 67 25.0 68 18.0 69 11.5 70 9.5 71 5.0 72 3.0 73 3.0 74 1.5 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08997357026373502 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.008435022212225158 20-21 0.0028116740707417195 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0028116740707417195 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0281167407074172 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.005623348141483439 64-65 0.005623348141483439 66-67 0.0028116740707417195 68-69 0.0028116740707417195 70-71 0.0 72-73 0.0 74-75 0.005623348141483439 76-77 0.005623348141483439 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.011246696282966878 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 17783.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.63712534443007 #Duplication Level Percentage of deduplicated Percentage of total 1 97.90916987219258 88.74205702075015 2 1.6751457997270132 3.0366079964010573 3 0.16131033627000868 0.4386211550357083 4 0.049633949621541135 0.17994714052747005 5 0.012408487405385284 0.0562334814148344 6 0.018612731108077925 0.1012202665467019 7 0.024816974810770567 0.1574537479615363 8 0.024816974810770567 0.17994714052747005 9 0.024816974810770567 0.2024405330934038 >10 0.049633949621541135 0.9222290952032841 >50 0.018612731108077925 1.0684361468818535 >100 0.03102121851346321 4.9148062756565265 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 349 1.9625485013777204 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 161 0.9053590507788337 RNA PCR Primer, Index 17 (96% over 28bp) ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 134 0.7535286509587809 RNA PCR Primer, Index 17 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.6579317325535624 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 113 0.6354383399876287 RNA PCR Primer, Index 17 (95% over 23bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78 0.4386211550357083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 0.32053084406455606 No Hit CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA 55 0.3092841477815892 RNA PCR Primer, Index 17 (96% over 28bp) CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 49 0.2755440589326885 RNA PCR Primer, Index 17 (96% over 26bp) CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 36 0.2024405330934038 TruSeq Adapter, Index 12 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.005623348141483439 0.0 24-25 0.0 0.0 0.0 0.008435022212225158 0.0 26-27 0.0 0.0 0.0 0.025305066636675477 0.0 28-29 0.0 0.0 0.0 0.059045155485576116 0.0 30-31 0.0 0.0 0.0 0.10403194061744363 0.0 32-33 0.0 0.0 0.0 0.17994714052747007 0.0 34-35 0.0 0.0 0.0 0.3402125625597481 0.0 36-37 0.0 0.0 0.0 0.5651464882190856 0.0 38-39 0.0 0.0 0.0 0.8913006804251251 0.0 40-41 0.0 0.0 0.0 1.214643198560423 0.0 42-43 0.0 0.0 0.0 1.5379857166957205 0.0 44-45 0.0 0.0 0.0 1.8303998200528595 0.0 46-47 0.0 0.0 0.0 2.164989034471124 0.0 48-49 0.0 0.0 0.0 2.555811730304223 0.0 50-51 0.0 0.0 0.0 2.8904009447224874 0.0 52-53 0.0 0.0 0.0 3.2587302479896527 0.0 54-55 0.0 0.0 0.0 3.6045661586908846 0.0 56-57 0.0 0.0 0.0 3.911038632401732 0.0 58-59 0.0 0.0 0.0 4.290614631951865 0.0 60-61 0.0 0.0 0.0 4.684249001855704 0.0 62-63 0.0 0.0 0.0 5.049766631052129 0.0 64-65 0.0 0.0 0.0 5.367485801045943 0.0 66-67 0.0 0.0 0.0 5.77236686723275 0.0 68-69 0.0 0.0 0.0 6.1800596074903 0.0 70-71 0.0 0.0 0.0 6.593375695889332 0.0 72-73 0.0 0.0 0.0 7.006691784288366 0.0 74-75 0.0 0.0 0.0 7.380644435697015 0.0 76-77 0.0 0.0 0.0 7.720856998256762 0.0 78-79 0.0 0.0 0.0 8.103244671877636 0.0 80-81 0.0 0.0 0.0 8.516560760276668 0.0 82-83 0.0 0.0 0.0 8.983298656019794 0.0 84-85 0.0 0.0 0.0 9.447224877692179 0.0 86-87 0.0 0.0 0.0 9.902716077152336 0.0 88-89 0.0 0.0 0.0 10.287915424843952 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 45 2.1482265E-9 74.28769 1 CAACTCT 20 0.0020426544 71.23099 4 AGCTACA 20 0.0020426544 71.23099 7 CAACGCA 50 5.173206E-9 66.48225 5 AACGCAG 55 1.0990334E-8 60.43841 6 ACGCAGA 55 1.0990334E-8 60.43841 7 TCAACGC 55 1.0990334E-8 60.43841 4 TATCAAC 55 1.0990334E-8 60.43841 2 CCAGTGT 25 0.0049456493 56.984787 3 ATACTAT 25 0.0049456493 56.984787 6 CAGTGTC 25 0.0049456493 56.984787 4 ATCAACG 60 2.1844244E-8 55.40188 3 CGCAGAG 65 4.1063686E-8 51.140194 8 GCAGAGT 65 2.4521105E-6 43.83445 9 GAGTACT 35 2.0168233E-4 33.919518 12-13 AGAGTAC 55 2.6419075E-6 30.219206 10-11 CAGAGTA 65 2.906072E-7 29.222967 10-11 AGTACTT 35 0.008295183 27.135614 12-13 TATACAC 180 1.7408274E-7 23.743662 3 CTTATAC 190 2.7767237E-7 22.621439 1 >>END_MODULE