FastQCFastQC Report
Fri 10 Feb 2017
SRR2031398_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031398_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066905
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.1975808530281515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14190.13300153247008872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11100.061.1452871
GTATCAA22650.048.6149331
TCAACGC30300.035.5609934
ATCAACG30750.035.349323
CAACGCA30900.034.870495
TATCAAC31350.034.8241842
AACGCAG31900.034.0621766
ACGCAGA35700.030.0377797
CGCAGAG36450.029.419728
GTACATG37000.028.7340091
TACATGG36500.028.2200372
ACATGGG38050.026.0724773
GCAGAGT44700.023.2468559
AGAGTAC37750.022.24763910-11
CATGGGG27800.021.1722764
CAGAGTA41400.020.22888610-11
TAAGACC2902.4592737E-919.6414684
GAGTACT23450.019.52594412-13
GTATATA5300.018.8058661
AGTACTT27200.018.75284612-13
Produced by FastQC (version 0.11.5)