FastQCFastQC Report
Fri 10 Feb 2017
SRR2031398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066905
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.25859846940449244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.17274265281351198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.10656993827941569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.062.7794571
GTATCAA25000.053.8073731
TCAACGC31100.042.6101884
ATCAACG31900.041.541593
CAACGCA32350.040.816915
AACGCAG32950.040.0736586
TATCAAC33400.039.818162
ACGCAGA36000.036.5465937
CGCAGAG38400.033.643988
GTACATG36200.030.9883771
TACATGG36550.030.6686212
ACATGGG38200.028.9709113
GCAGAGT45750.027.9274189
AGAGTAC39000.023.2006410-11
GAGTACT25700.022.82568612-13
CATGGGG26100.022.5658024
CAGAGTA42350.022.26264210-11
ACCGTGC2154.4234184E-822.0918058
CATGGGA14700.021.9715754
TATACGA1100.002878154921.5897162
Produced by FastQC (version 0.11.5)