FastQCFastQC Report
Fri 10 Feb 2017
SRR2031396_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031396_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480952
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25010.16887785694607252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.12512221868095658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.054.5446551
GTATCAA26950.044.0330731
TCAACGC31450.036.833284
ATCAACG32500.035.935443
CAACGCA32750.035.5161555
AACGCAG34800.033.547926
TATCAAC34950.033.4140972
ACGCAGA39000.029.6916927
GTACATG40450.029.454591
CGCAGAG40800.028.2654468
TACATGG41150.028.1489242
ACATGGG42000.025.43343
GCAGAGT49500.022.9148569
CATGGGG29150.022.3127544
AGAGTAC42850.020.71027410-11
TAGTGCG1200.00477826919.7815325
CAGAGTA47500.019.38220610-11
GAGTACT28450.019.09933712-13
TTACGCT1300.00758649818.2598784
TATACTG8500.017.8731985
Produced by FastQC (version 0.11.5)