FastQCFastQC Report
Fri 10 Feb 2017
SRR2031396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480952
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29860.2016270615117843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21660.14625727234913757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.059.6256521
GTATCAA30200.048.645621
TCAACGC36950.038.9488754
CAACGCA37600.038.401885
ATCAACG38000.037.8726583
AACGCAG39650.037.015366
TATCAAC40200.035.918182
ACGCAGA44950.032.545267
CGCAGAG46450.031.2897788
GTACATG39250.026.648651
GCAGAGT55850.025.7683269
TACATGG40700.024.8571852
ACATGGG39350.024.3822273
AGAGTAC47500.022.54860510-11
CAGAGTA51700.021.9111210-11
GAGTACT33700.021.14219712-13
CATGGGG27500.020.725994
TATACTG7600.018.748845
GTATATA11450.018.6852631
GTACTTT37450.018.45595714-15
Produced by FastQC (version 0.11.5)