FastQCFastQC Report
Fri 10 Feb 2017
SRR2031393_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031393_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823492
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.038.6936841
TCAACGC33800.036.2362564
ATCAACG35250.035.015033
CAACGCA35250.034.7456865
GTATCAA37500.034.3031
AACGCAG37350.032.9081276
TATCAAC43650.028.166992
ACGCAGA42800.028.0524487
CGCAGAG43650.027.2887388
GTACATG62250.025.697131
ACATGGG62600.024.3428253
TACATGG69100.022.326992
CATGGGG35850.021.054664
GCAGAGT58800.019.3688959
ACCGTAT3054.622052E-918.6713358
TAATACT29750.017.7123554
AGAGTAC47600.017.39578410-11
CATGGGA30650.016.7275964
GAGTACA43350.016.4246541
CAGAGTA55100.016.27667610-11
Produced by FastQC (version 0.11.5)