FastQCFastQC Report
Fri 10 Feb 2017
SRR2031393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823492
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.039.8147931
GTATCAA38000.037.0283931
TCAACGC36100.036.971264
CAACGCA36400.036.405585
ATCAACG36700.036.2374043
AACGCAG39700.033.498476
CGCAGAG45300.029.252538
TATCAAC46950.029.2366332
ACGCAGA45900.029.0770977
GTACATG64300.026.2448521
ACATGGG63450.025.0771563
ATAACGC1401.7815766E-523.7484423
TACATGG71400.023.2162652
CATGGGG38500.022.3297044
GCAGAGT62250.021.2110849
AGAGTAC50500.019.84488110-11
TAATACT28400.019.5673774
CCTATAC12300.019.3076743
TAGCGGT2001.2865454E-518.9987534
GTATATA19650.018.3855971
Produced by FastQC (version 0.11.5)