FastQCFastQC Report
Fri 10 Feb 2017
SRR2031391_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031391_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107615
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.22047372056174752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.18986741783020275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.12368918803013682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.057.7464331
GTATCAA25300.046.7219731
TCAACGC32350.035.9626774
ATCAACG33250.034.9892543
CAACGCA34650.033.5755425
TATCAAC34600.033.485312
AACGCAG35050.033.4451756
GTACATG38200.029.825671
TACATGG36350.029.7831752
ACATGGG36450.029.1817053
ACGCAGA40450.028.7456477
CGCAGAG40500.028.5929768
CATGGGG25350.026.2246864
GTATAGG2750.025.8941061
GCAGAGT46550.024.2639889
AGAGTAC40550.022.46860110-11
AACTCGC1150.003741565920.6346327
CAGAGTA44250.020.4826310-11
ATGGGGA12100.020.0145135
GTACGCG600.00476273219.793238-39
Produced by FastQC (version 0.11.5)