FastQCFastQC Report
Fri 10 Feb 2017
SRR2031391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107615
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.230314685156846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.19176338348613914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12910.11655674580066178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.061.813741
GTATCAA26850.048.891851
ATCAACG33850.038.164023
ACCGTCG500.001616700137.9956478
TCAACGC34050.037.9398544
CAACGCA35100.036.9402165
AACGCAG35800.036.217926
TATCAAC36850.035.185932
ACGCAGA40800.031.3138147
CGCAGAG42050.030.2700188
GTACATG37700.028.0080781
TACATGG37350.027.2124122
GCAGAGT48400.026.102389
ACATGGG37400.025.7791333
GTACCGT950.001227345924.9971376
GTATAGG2801.8189894E-1223.7816351
AGAGTAC42050.023.54962210-11
GTATTAC2606.148184E-1021.9522761
CAGAGTA45950.021.5508510-11
GAGTACT26200.021.48180212-13
Produced by FastQC (version 0.11.5)