FastQCFastQC Report
Fri 10 Feb 2017
SRR2031390_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031390_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283200
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.2790679551122195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27350.21313902743142146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.12531172069825436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.055.110081
GTATCAA33250.049.2643971
TCAACGC41000.039.031894
ATCAACG41450.038.7227063
CAACGCA41600.038.1264765
TATCAAC42700.037.8086052
AACGCAG43400.036.7410856
ACGCAGA48050.033.2842647
CGCAGAG49200.032.409828
GCAGAGT56550.028.0295629
GTACATG41650.026.5611361
ACATGGG40750.025.520553
TACATGG43050.024.7066862
AGAGTAC50250.024.22349410-11
CATGGGG24700.023.6473064
CAGAGTA53100.023.05741910-11
TGGACCG1553.915211E-521.4456835
GAGTACT36050.021.39112312-13
AGTACTT37950.020.1951112-13
GGACCGT1951.0408599E-519.4696356
Produced by FastQC (version 0.11.5)