FastQCFastQC Report
Fri 10 Feb 2017
SRR2031390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283200
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42840.33385286783042395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32840.2559226932668329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.15492518703241895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.063.902091
GTATCAA36200.043.6113551
TCAACGC43450.035.417454
ATCAACG44000.034.9747353
CAACGCA45100.034.1216935
TATCAAC45250.034.0085832
AACGCAG47250.033.2714236
ACGCAGA52450.029.7011647
CGCAGAG53300.029.2275098
TAACGCT700.00839490227.1408844
TACATGG43250.025.2582842
GTACATG45450.024.9007911
GCAGAGT62700.024.7699649
ACATGGG43500.023.802873
CAGAGTA59350.022.68702110-11
AGAGTAC56050.022.32801810-11
GAGTACT39350.022.08861212-13
CATGGGG25800.020.4345634
GTACTTT44150.020.01143314-15
AGTACTT43550.019.52211812-13
Produced by FastQC (version 0.11.5)