##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031390_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1283200 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.29532185162095 34.0 33.0 34.0 31.0 34.0 2 33.3929457605985 34.0 34.0 34.0 31.0 34.0 3 33.48118220074813 34.0 34.0 34.0 31.0 34.0 4 36.654695293017454 37.0 37.0 37.0 35.0 37.0 5 36.56483245012469 37.0 37.0 37.0 35.0 37.0 6 36.61595230673317 37.0 37.0 37.0 37.0 37.0 7 36.6268305798005 37.0 37.0 37.0 37.0 37.0 8 36.63427213216958 37.0 37.0 37.0 37.0 37.0 9 38.57276885910225 39.0 39.0 39.0 38.0 39.0 10-11 38.57914004052369 39.0 39.0 39.0 38.0 39.0 12-13 38.577847178927684 39.0 39.0 39.0 38.0 39.0 14-15 40.28585995947631 41.0 41.0 41.0 39.0 41.0 16-17 40.3421789276808 41.0 41.0 41.0 39.0 41.0 18-19 40.37265001558603 41.0 41.0 41.0 39.0 41.0 20-21 40.36816630299252 41.0 41.0 41.0 39.0 41.0 22-23 40.32523729738155 41.0 41.0 41.0 39.0 41.0 24-25 40.29865336658354 41.0 41.0 41.0 39.0 41.0 26-27 40.22403990024938 41.0 40.0 41.0 39.0 41.0 28-29 40.15444046134664 41.0 40.0 41.0 38.5 41.0 30-31 40.01389261221945 41.0 40.0 41.0 38.0 41.0 32-33 39.88531055174563 41.0 40.0 41.0 38.0 41.0 34-35 39.80916692643392 41.0 40.0 41.0 38.0 41.0 36-37 39.72386338840399 41.0 40.0 41.0 37.0 41.0 38-39 39.62570176122195 41.0 40.0 41.0 37.0 41.0 40-41 39.525156249999995 41.0 40.0 41.0 37.0 41.0 42-43 39.33915601620947 41.0 40.0 41.0 36.0 41.0 44-45 39.20349555798005 41.0 40.0 41.0 36.0 41.0 46-47 39.039627104114714 41.0 39.5 41.0 35.0 41.0 48-49 38.82189876870324 41.0 39.0 41.0 35.0 41.0 50-51 38.718449968827926 41.0 39.0 41.0 35.0 41.0 52-53 38.72780587593516 41.0 39.0 41.0 35.0 41.0 54-55 38.66254286159601 41.0 39.0 41.0 35.0 41.0 56-57 38.59565889962594 41.0 39.0 41.0 35.0 41.0 58-59 38.559681265586036 41.0 38.5 41.0 35.0 41.0 60-61 38.393998597256854 41.0 37.5 41.0 35.0 41.0 62-63 38.205347958229424 40.0 37.0 41.0 35.0 41.0 64-65 37.95248870012469 40.0 37.0 41.0 35.0 41.0 66-67 37.67615765274314 39.0 36.0 41.0 35.0 41.0 68-69 37.346815383416455 39.0 35.5 41.0 34.5 41.0 70-71 37.007722880299255 38.5 35.0 41.0 34.0 41.0 72-73 36.667164900249375 37.0 35.0 40.0 34.0 41.0 74-75 36.28757208541147 37.0 35.0 39.0 34.0 41.0 76-77 35.09226737842893 35.5 34.5 39.0 32.0 39.5 78-79 35.47605478491272 36.0 35.0 38.5 33.0 40.0 80-81 35.24951527431422 35.5 35.0 37.0 34.0 39.0 82-83 34.93779847256858 35.0 35.0 37.0 33.0 39.0 84-85 34.64759780236908 35.0 35.0 36.5 33.0 38.0 86-87 34.42341684850374 35.0 35.0 36.0 33.0 37.0 88-89 34.22130727867831 35.0 35.0 36.0 33.0 37.0 90-91 34.05405860349127 35.0 35.0 35.5 33.0 36.5 92-93 33.94601075436409 35.0 35.0 35.0 33.0 36.0 94-95 33.84667471945137 35.0 35.0 35.0 33.0 36.0 96-97 33.767094373441395 35.0 35.0 35.0 33.0 36.0 98-99 33.702186331047386 35.0 35.0 35.0 32.5 36.0 100-101 33.453185395885285 35.0 34.5 35.0 31.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05459571648399475 1101 2 0.023813738090154857 1101 3 0.022274805022583166 1101 4 0.07084302079353932 1101 5 0.006818022747218322 1101 6 0.026819320954061254 1101 7 0.03818838055103413 1101 8 0.05957131102571367 1101 9 0.10485476758410073 1101 10-11 0.008024239063324501 1101 12-13 0.05749651584588378 1101 14-15 0.10917567989535115 1101 16-17 0.10018419439488468 1101 18-19 0.11065908912852507 1101 20-21 0.07533702413136467 1101 22-23 0.02057239319842097 1101 24-25 0.011587819671490251 1101 26-27 -0.009723307182227359 1101 28-29 0.07377340668731591 1101 30-31 0.041632374952371265 1101 32-33 0.07578348369594323 1101 34-35 0.023324790751878766 1101 36-37 0.0913853779889564 1101 38-39 0.07591602784505369 1101 40-41 0.09953453367671017 1101 42-43 0.07730168025901918 1101 44-45 0.14136647434651195 1101 46-47 0.1440223425131606 1101 48-49 0.22383765030649272 1101 50-51 0.27340154420080864 1101 52-53 0.21141272264669908 1101 54-55 0.12597794451517075 1101 56-57 0.0940968413269303 1101 58-59 0.17132966039319086 1101 60-61 0.28433804333363355 1101 62-63 0.13813977021316504 1101 64-65 0.13930190370937368 1101 66-67 0.11580258192716997 1101 68-69 0.14938811607840563 1101 70-71 0.003436121160355299 1101 72-73 0.090949280168374 1101 74-75 0.08265227043159484 1101 76-77 -0.04876138269470687 1101 78-79 0.05274814720494447 1101 80-81 0.06368721712103564 1101 82-83 0.014318870743146306 1101 84-85 0.012064005556609914 1101 86-87 0.16193041802754493 1101 88-89 0.15525381660552284 1101 90-91 0.02503051360747577 1101 92-93 -0.04801339377566194 1101 94-95 0.035689067665082064 1101 96-97 0.15377422492047543 1101 98-99 -0.029004837623226365 1101 100-101 0.0644886930678652 1102 1 0.015404508952769902 1102 2 0.05381720800748013 1102 3 0.03437802751971475 1102 4 0.027165059294532057 1102 5 0.008641992332641735 1102 6 0.07021667174762314 1102 7 -0.005509193524162015 1102 8 0.019357986543184325 1102 9 0.012888380522369403 1102 10-11 0.01788710189597964 1102 12-13 0.10075205262700138 1102 14-15 0.11844714812297497 1102 16-17 0.08585117285122834 1102 18-19 0.013683574675567911 1102 20-21 0.003919713769896305 1102 22-23 -0.03296201932948861 1102 24-25 -7.733836354901769E-4 1102 26-27 -0.09931053788646693 1102 28-29 0.06370234881512715 1102 30-31 0.03165673980662831 1102 32-33 0.11850231478640438 1102 34-35 0.13654593009169247 1102 36-37 0.0714431596557219 1102 38-39 0.03407749969346696 1102 40-41 0.11626896430853861 1102 42-43 0.2176583274136874 1102 44-45 0.1977322639789989 1102 46-47 0.19156096420164914 1102 48-49 0.06811756702848015 1102 50-51 0.187722873631742 1102 52-53 0.2961956265149084 1102 54-55 0.17019336603469526 1102 56-57 0.19261194301058993 1102 58-59 0.155636204958995 1102 60-61 0.3037699575210695 1102 62-63 0.23278893139837464 1102 64-65 0.17860776937831702 1102 66-67 0.14551789784052005 1102 68-69 0.19865981060498683 1102 70-71 0.2024792537413731 1102 72-73 0.2115575717621283 1102 74-75 0.19297112566061259 1102 76-77 -0.03066274652308465 1102 78-79 0.18925884360226775 1102 80-81 0.18162970701300907 1102 82-83 0.27607887315701163 1102 84-85 0.2482526031065504 1102 86-87 0.22050752562515186 1102 88-89 0.2458559981274533 1102 90-91 0.16611546623555995 1102 92-93 0.24168170609760864 1102 94-95 0.19207899585288146 1102 96-97 0.2610067395544746 1102 98-99 0.2164397643923408 1102 100-101 0.25351922834033047 1103 1 0.12264231397026037 1103 2 0.06751726746730924 1103 3 0.05383254074953214 1103 4 0.05177085205415466 1103 5 0.06606360826787494 1103 6 0.08718130458316864 1103 7 0.0681766870530609 1103 8 0.10811035921910417 1103 9 0.08916562341577361 1103 10-11 0.071305184545146 1103 12-13 0.12381703536078703 1103 14-15 0.09778628641281983 1103 16-17 0.06458999554658362 1103 18-19 0.07096297298782162 1103 20-21 0.07494062190445305 1103 22-23 0.14285255175931155 1103 24-25 0.10179911161915811 1103 26-27 0.12122254236142993 1103 28-29 0.0735060742021929 1103 30-31 0.1592388115243324 1103 32-33 0.13685553476490497 1103 34-35 0.10634862636630515 1103 36-37 0.0503592183837398 1103 38-39 0.05199620552738793 1103 40-41 0.15124663828571272 1103 42-43 0.20218161965875225 1103 44-45 0.230419448098651 1103 46-47 -0.01366263906406573 1103 48-49 0.011286261427819966 1103 50-51 0.1418165971892691 1103 52-53 0.1261991140626435 1103 54-55 0.05851822824797637 1103 56-57 0.16276267939771571 1103 58-59 0.1910269532380866 1103 60-61 0.2234127125694414 1103 62-63 0.2772773195795821 1103 64-65 0.28883857643116073 1103 66-67 0.1761954540414905 1103 68-69 0.2626098586302277 1103 70-71 0.15665587802384806 1103 72-73 0.1582038086409412 1103 74-75 0.08253092281111662 1103 76-77 -0.054230850897951655 1103 78-79 0.0945097469343068 1103 80-81 0.17469749121956824 1103 82-83 0.08056180132496138 1103 84-85 0.23468761465547772 1103 86-87 0.0990057932858619 1103 88-89 0.10542002607357404 1103 90-91 0.17558687406138773 1103 92-93 0.21197608943055712 1103 94-95 0.10621814329039125 1103 96-97 0.07843647404346399 1103 98-99 0.16911059705866194 1103 100-101 0.13722666310603415 1104 1 -0.07864525376137976 1104 2 -0.05920841101129071 1104 3 -0.1316540537520936 1104 4 0.023679397418462145 1104 5 9.414212759040197E-4 1104 6 -0.03668607470991958 1104 7 -0.030144429631995706 1104 8 -0.0017382965394006078 1104 9 -0.0478228599833912 1104 10-11 -0.005707554404153825 1104 12-13 0.10076537541429786 1104 14-15 0.11710014420747683 1104 16-17 0.005249010913452423 1104 18-19 0.04031196988272967 1104 20-21 0.0774905994126911 1104 22-23 0.028317543234322784 1104 24-25 0.02780259271446539 1104 26-27 0.02377238314165453 1104 28-29 -0.09799632058901864 1104 30-31 0.021069681702442722 1104 32-33 -0.05268743816704813 1104 34-35 -0.013559109568141992 1104 36-37 0.07637890174900264 1104 38-39 -0.0023753993450554844 1104 40-41 -0.09645633103662732 1104 42-43 0.1159199140314442 1104 44-45 0.025399205515803658 1104 46-47 -0.06037644955083721 1104 48-49 -0.0645318164770714 1104 50-51 0.012394642224741403 1104 52-53 -0.04986657828017371 1104 54-55 0.018945722064444226 1104 56-57 0.10837090714284159 1104 58-59 0.22767006166751003 1104 60-61 0.02493558921752026 1104 62-63 -0.03642357968057297 1104 64-65 -0.05796075742993878 1104 66-67 0.02176252665363876 1104 68-69 0.09805769073916792 1104 70-71 0.18390132805728143 1104 72-73 0.2446822281744545 1104 74-75 0.22641938556435548 1104 76-77 -0.030619973363869235 1104 78-79 0.1825157602324694 1104 80-81 0.2909621091536323 1104 82-83 0.19140344430349643 1104 84-85 0.2091372502069504 1104 86-87 0.18074145974607347 1104 88-89 0.17488025120992745 1104 90-91 0.1029398606798182 1104 92-93 0.10060530902805453 1104 94-95 0.08937222619405816 1104 96-97 -0.014808615028002237 1104 98-99 -0.022343149904266113 1104 100-101 -0.07953783245645951 1105 1 0.08056152422992824 1105 2 0.06894097680660138 1105 3 0.03059860573814177 1105 4 0.0463257073575889 1105 5 0.0293291402713578 1105 6 0.08424033350930671 1105 7 0.03648746020431304 1105 8 0.03677995192359873 1105 9 0.015141302428283154 1105 10-11 0.0404467293191928 1105 12-13 0.13649404373965268 1105 14-15 0.18835192264157996 1105 16-17 0.1392813714651382 1105 18-19 0.12046997821753536 1105 20-21 0.11861904938092493 1105 22-23 0.11716846633938616 1105 24-25 0.10057634228378731 1105 26-27 0.10980980895809012 1105 28-29 0.12345613567006808 1105 30-31 0.19387691528316253 1105 32-33 0.16582482454411007 1105 34-35 0.14524204647057815 1105 36-37 0.20845716642394763 1105 38-39 0.19827624605797212 1105 40-41 0.17465944284158041 1105 42-43 0.15010851175595263 1105 44-45 0.18543985174429878 1105 46-47 0.1259484992050588 1105 48-49 0.11134812459624044 1105 50-51 0.2577813793241859 1105 52-53 0.15427438552929829 1105 54-55 0.16036977509071448 1105 56-57 0.11403571667505474 1105 58-59 0.12885150428541436 1105 60-61 0.0833008713397092 1105 62-63 0.1280378610239552 1105 64-65 0.18992305951465482 1105 66-67 0.2052500609699024 1105 68-69 0.1590150749009709 1105 70-71 0.1446591009697542 1105 72-73 0.16591529305392783 1105 74-75 0.1768982780321906 1105 76-77 -0.012015661941880751 1105 78-79 0.15793991797706752 1105 80-81 0.09370088150566147 1105 82-83 0.20409213931262826 1105 84-85 0.1994934670825046 1105 86-87 0.19442627177343041 1105 88-89 0.07379635178348565 1105 90-91 0.07526642416660678 1105 92-93 0.07705014735134341 1105 94-95 0.038572843902962006 1105 96-97 0.11548717548878784 1105 98-99 0.10968275560230012 1105 100-101 0.17707800703229992 1106 1 0.0811912252075544 1106 2 0.12298215925054024 1106 3 0.09530496639050767 1106 4 0.09057228847866128 1106 5 0.13601882668638865 1106 6 -0.07755491340989806 1106 7 0.07057934886095296 1106 8 0.06299385339801944 1106 9 0.13973467149357788 1106 10-11 0.10826583028960357 1106 12-13 0.1549662913503056 1106 14-15 0.10858500982092778 1106 16-17 0.0860985387033324 1106 18-19 0.08013977036119257 1106 20-21 0.05828880484296661 1106 22-23 0.04226339529016343 1106 24-25 0.0953657195339801 1106 26-27 0.07645583202959472 1106 28-29 0.08020423754957307 1106 30-31 0.22701508207077836 1106 32-33 -0.023676348350377907 1106 34-35 0.10670618072037996 1106 36-37 0.10908057039179653 1106 38-39 0.0990760274355722 1106 40-41 0.040129025533239826 1106 42-43 0.2418113149034795 1106 44-45 0.11016674187126085 1106 46-47 -0.1714268704385873 1106 48-49 0.10568998620019698 1106 50-51 0.13409588011498386 1106 52-53 -0.09167336640764745 1106 54-55 0.13752416248642874 1106 56-57 0.11595538761655177 1106 58-59 0.15864339116911452 1106 60-61 0.2710443837383991 1106 62-63 0.262082552675281 1106 64-65 0.297726032803709 1106 66-67 0.22218871158796105 1106 68-69 0.01295868426138469 1106 70-71 0.12170833441579987 1106 72-73 0.16518808012879305 1106 74-75 0.22843960873097302 1106 76-77 0.1314892170179789 1106 78-79 0.30654109533448803 1106 80-81 0.25595220311351596 1106 82-83 0.2500596993191593 1106 84-85 0.28606940382384494 1106 86-87 0.23004105861964774 1106 88-89 0.08947070956490677 1106 90-91 0.28940877449188207 1106 92-93 0.2719233420811804 1106 94-95 0.26792614099893797 1106 96-97 0.20527580993636008 1106 98-99 0.20015672480320035 1106 100-101 0.2822521315798241 1107 1 -0.03194151257065414 1107 2 -0.02773081062098015 1107 3 -0.023194766119758015 1107 4 -0.0045366231405949975 1107 5 0.023832682298866814 1107 6 -0.01868937830587214 1107 7 -0.03547232469955475 1107 8 0.001361489868195065 1107 9 0.0013635950223545024 1107 10-11 0.058604077288791245 1107 12-13 -0.007359351010578052 1107 14-15 0.014421208833290677 1107 16-17 -0.0517185789791057 1107 18-19 -0.08481925210913488 1107 20-21 -0.07764076054255753 1107 22-23 -0.08450953973058972 1107 24-25 -0.09146518543539628 1107 26-27 -0.11078550065052895 1107 28-29 -0.15457761300492479 1107 30-31 -0.054576582688071085 1107 32-33 0.006665350511923407 1107 34-35 0.08133053853742922 1107 36-37 0.011114382043437843 1107 38-39 0.06902880452469162 1107 40-41 -0.0945618429246764 1107 42-43 0.11192399273077314 1107 44-45 0.077542780247434 1107 46-47 -0.035503532641165236 1107 48-49 0.004728624529157344 1107 50-51 0.035747139926399996 1107 52-53 -0.03865642618181653 1107 54-55 -0.047509045385723425 1107 56-57 -0.051333939009168716 1107 58-59 -0.0324715013071355 1107 60-61 -0.02213563626105497 1107 62-63 -0.06126420061615079 1107 64-65 -0.020427505118739475 1107 66-67 0.053142612015349755 1107 68-69 -0.06947500971849507 1107 70-71 -0.003281434546615003 1107 72-73 0.004572948631789586 1107 74-75 -0.13674852967093898 1107 76-77 -0.2544599207872764 1107 78-79 0.0664844776621365 1107 80-81 0.038672544076931104 1107 82-83 0.13637329930397613 1107 84-85 0.09736903621821824 1107 86-87 0.11754294172366286 1107 88-89 0.05595205111787038 1107 90-91 0.03219129643967733 1107 92-93 0.026519598648320653 1107 94-95 0.021893707720131772 1107 96-97 0.14247058398166956 1107 98-99 0.19452431619750143 1107 100-101 0.08961029381264751 1108 1 -0.022874344598783125 1108 2 0.01009662109461118 1108 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0.12831240683824774 2315 100-101 -0.3666624274071424 2316 1 -0.1517181297579171 2316 2 -0.1440856296188855 2316 3 -0.18175411197342584 2316 4 -0.10249772226163856 2316 5 -0.0192382800082882 2316 6 -0.021811033305375815 2316 7 -0.06495351641726188 2316 8 -0.39336701735057744 2316 9 -0.3297192171369261 2316 10-11 -0.18461223060173637 2316 12-13 -0.09605311458231824 2316 14-15 -0.1502572855754991 2316 16-17 -0.24039381161080797 2316 18-19 -0.2404408013952164 2316 20-21 -0.2745879643605065 2316 22-23 -0.3111707120633582 2316 24-25 -0.28765705652970297 2316 26-27 -0.2482210258306381 2316 28-29 -0.2643384021991082 2316 30-31 -0.5446811850771169 2316 32-33 -0.8694731415255745 2316 34-35 -0.7347183748530242 2316 36-37 -0.4982565941101029 2316 38-39 -0.3969488870425266 2316 40-41 -0.4201268293145901 2316 42-43 -0.41736069640131035 2316 44-45 -0.3926823105624564 2316 46-47 -0.2971865233939326 2316 48-49 -0.39029194191234495 2316 50-51 -0.32625643194338494 2316 52-53 -0.20849311346329102 2316 54-55 -0.2988509839293343 2316 56-57 -0.29497413697102104 2316 58-59 -0.3480125757368384 2316 60-61 -0.583713493984888 2316 62-63 -0.5685876529924627 2316 64-65 -0.33063481949219664 2316 66-67 -0.2959834074972747 2316 68-69 -0.31066783845197676 2316 70-71 -0.2596694268291273 2316 72-73 -0.3232230054048415 2316 74-75 -0.3219936728649486 2316 76-77 -0.21262062342082544 2316 78-79 -0.252374339790272 2316 80-81 -0.2602391881223767 2316 82-83 -0.21001204807030405 2316 84-85 -0.1591437393517836 2316 86-87 -0.21922559398384323 2316 88-89 -0.26786928574642843 2316 90-91 -0.2044324326155902 2316 92-93 -0.29609660526995896 2316 94-95 -0.3440936016951568 2316 96-97 -0.2792084186806463 2316 98-99 -0.5938658218600708 2316 100-101 -0.6771838369784788 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 10.0 14 21.0 15 62.0 16 193.0 17 452.0 18 974.0 19 1677.0 20 2611.0 21 4031.0 22 6029.0 23 7215.0 24 6742.0 25 5216.0 26 4262.0 27 3852.0 28 4240.0 29 5057.0 30 5912.0 31 7380.0 32 9490.0 33 12414.0 34 19249.0 35 34465.0 36 77925.0 37 239510.0 38 538852.0 39 284511.0 40 845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.249500748876684 19.68180541687469 14.074513230154768 22.99418060409386 2 14.453388321421585 21.13721538637782 41.53533350945731 22.87406278274328 3 17.07161783042394 27.48262157107232 31.263403990024933 24.182356608478802 4 11.431187655860349 18.32138403990025 41.066786159601 29.1806421446384 5 12.18255044435924 38.982119645011366 36.26897216013766 12.566357750491742 6 28.213450748129677 39.80985037406484 19.26737842892768 12.709320448877806 7 25.400483167082292 33.82309850374065 23.938435162094763 16.837983167082292 8 24.73293329177057 36.18227867830424 21.43290211970075 17.651885910224436 9 25.88497506234414 16.0321072319202 21.085567331670823 36.99735037406484 10-11 23.328475685785534 26.964463840399 30.187422069825438 19.519638403990026 12-13 24.839736596009978 24.65001558603491 29.691162718204488 20.819085099750623 14-15 21.818461658354117 25.838450748129677 27.71715243142145 24.625935162094763 16-17 21.111245324189525 29.065889962593516 28.00981920199501 21.813045511221947 18-19 21.09859193347582 28.05892564543689 30.064782360493865 20.777700060593425 20-21 22.12933016404941 27.203912247204148 29.972957175700426 20.69380041304602 22-23 21.743375935162096 27.194357855361595 29.63750779301746 21.424758416458854 24-25 21.533860660847882 27.230868142144637 29.777743142144637 21.45752805486284 26-27 21.511964101461068 27.501184564501095 29.586797405180288 21.40005392885755 28-29 21.461151807980052 27.48090710723192 29.719724127182047 21.338216957605987 30-31 21.8525171446384 27.382831982543642 29.550498753117203 21.21415211970075 32-33 21.560590710723194 27.38388403990025 29.62725997506234 21.428265274314214 34-35 21.72268547381546 27.538965087281795 29.541653678304243 21.1966957605985 36-37 21.76825181582967 27.416651080145886 29.407673244178433 21.407423859846006 38-39 21.626479733784297 27.603824902390173 29.458606420037874 21.311088943787652 40-41 21.798550498753116 27.484881546134666 29.377337905236907 21.33923004987531 42-43 21.857738466334165 27.39600218204489 29.206904613466335 21.539354738154614 44-45 21.76652119700748 27.564798940149625 29.129052369077307 21.539627493765586 46-47 21.875062112733197 27.588342331479144 28.796339996235236 21.74025555955242 48-49 21.837239659438502 27.797377598534883 28.698618660743858 21.666764081282754 50-51 21.888832605985037 27.923706359102248 28.4869466957606 21.700514339152118 52-53 22.001129987531172 27.95164432668329 28.444474750623446 21.602750935162096 54-55 21.883182668329177 27.81709788029925 28.53315928927681 21.766560162094763 56-57 22.039198877805486 28.0147677680798 28.299602556109726 21.646430798004985 58-59 21.93146041147132 27.892495324189525 28.50974127182045 21.666302992518705 60-61 22.08588550651391 27.993353796180227 28.438785057108017 21.48197564019784 62-63 21.850812388110068 27.984270526370757 28.52710960984951 21.637807475669664 64-65 21.921019672210363 27.787961614210815 28.545085688410452 21.745933025168366 66-67 21.94246804982034 27.921283143063004 28.37961532361673 21.756633483499925 68-69 22.031447388827104 27.870747117075904 28.380972272337257 21.716833221759735 70-71 21.961543452111673 27.770082104071225 28.342796089584652 21.925578354232446 72-73 22.0156767759895 27.90650824675102 28.178880150127966 21.898934827131512 74-75 21.91271914511584 28.019791640689295 28.181150091024715 21.886339123170153 76-77 21.934932561073786 27.88722537545951 28.19977383975978 21.978068223706924 78-79 21.966684850374065 27.926901496259354 28.08615180798005 22.020261845386532 80-81 21.992752493765586 28.0303927680798 27.998246571072322 21.978608167082296 82-83 22.123207605985037 27.96621726932668 28.045628117206984 21.864947007481298 84-85 22.115336658354114 27.91918640897756 28.053849750623446 21.91162718204489 86-87 22.00109414561956 28.13228407573919 28.0408325170103 21.82578926163095 88-89 22.06220154422552 27.914126565705565 28.060210377834537 21.96346151223437 90-91 22.026555651843218 28.015799662092654 28.058154976028526 21.8994897100356 92-93 22.168952618453865 27.983751558603494 27.982699501246884 21.86459632169576 94-95 22.007393860843127 28.007433608592997 28.044648413616645 21.940524116947234 96-97 22.032691708229425 27.959515274314217 28.12807824189526 21.879714775561094 98-99 22.045394326683294 27.939331359102244 27.96208697007481 22.05318734413965 100-101 21.95616427680798 28.0625 28.065227556109722 21.916108167082292 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25.0 1 25.0 2 67.5 3 108.0 4 88.5 5 81.0 6 147.5 7 324.5 8 659.0 9 912.5 10 791.5 11 677.0 12 781.5 13 886.0 14 982.5 15 1064.0 16 1140.0 17 1250.0 18 1429.5 19 1635.5 20 1834.5 21 2093.0 22 2632.0 23 3468.5 24 4478.0 25 5871.0 26 8018.5 27 10731.5 28 13847.0 29 17753.0 30 21735.0 31 25484.0 32 29324.5 33 32503.0 34 35095.0 35 37171.5 36 39499.5 37 41483.5 38 41917.0 39 41852.5 40 42274.5 41 42773.5 42 42885.5 43 43320.0 44 44075.0 45 45207.5 46 45713.5 47 45566.5 48 45477.5 49 44880.5 50 43716.5 51 42312.5 52 40132.0 53 38339.0 54 36826.5 55 34197.5 56 30908.0 57 27447.5 58 24656.5 59 21435.0 60 17651.0 61 14213.5 62 11067.5 63 8341.0 64 6005.0 65 4181.5 66 2945.0 67 2079.0 68 1399.5 69 945.5 70 693.0 71 524.0 72 372.5 73 260.0 74 194.0 75 128.5 76 82.0 77 46.0 78 27.5 79 13.0 80 9.0 81 6.0 82 1.0 83 3.0 84 3.5 85 2.5 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0997506234413965 2 0.0010130922693266833 3 0.0 4 0.0 5 3.1172069825436414E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.004480985037406484 20-21 0.003896508728179551 22-23 0.0 24-25 0.0 26-27 0.0021820448877805485 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0031172069825436406 38-39 0.0023379052369077308 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.01952150872817955 48-49 0.0019482543640897754 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.004792705735660848 62-63 0.010208852867830423 64-65 0.009429551122194514 66-67 0.005416147132169576 68-69 0.003195137157107232 70-71 8.961970074812968E-4 72-73 0.0021820448877805485 74-75 0.002493765586034913 76-77 0.00303927680798005 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 5.455112219451371E-4 88-89 0.002532730673316708 90-91 6.234413965087283E-4 92-93 0.0 94-95 0.008455423940149625 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1283200.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.21619234235456 #Duplication Level Percentage of deduplicated Percentage of total 1 73.69988134715852 45.85325993503524 2 15.344208208576077 19.0931641849181 3 5.223592922928727 9.749761860332873 4 2.2814009066598198 5.6776031047507765 5 1.1969372145448127 3.7234437980921085 6 0.6688589176983689 2.4968313044052493 7 0.4177458407217713 1.8193388903595034 8 0.26179647378956517 1.3030383814273416 9 0.1871978263421104 1.0482062372794285 >10 0.6919597929693838 6.893756104437948 >50 0.017135243224920882 0.7054861665164713 >100 0.008908881465641467 0.8901156994125301 >500 0.0 0.0 >1k 3.764239203294671E-4 0.7459943330324103 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4284 0.33385286783042395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3284 0.2559226932668329 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1988 0.15492518703241895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.896508728179551E-5 0.0 12-13 0.0 0.0 0.0 7.793017456359102E-5 0.0 14-15 0.0 0.0 0.0 1.5586034912718204E-4 0.0 16-17 0.0 0.0 0.0 3.8965087281795513E-4 0.0 18-19 0.0 0.0 0.0 4.675810473815461E-4 0.0 20-21 0.0 0.0 0.0 7.403366583541147E-4 0.0 22-23 0.0 0.0 0.0 0.0011299875311720697 0.0 24-25 0.0 0.0 0.0 0.0015586034912718205 0.0 26-27 0.0 0.0 0.0 0.001753428927680798 0.0 28-29 0.0 0.0 0.0 0.002065149625935162 0.0 30-31 0.0 0.0 0.0 0.0023379052369077308 0.0 32-33 0.0 0.0 0.0 0.0031951371571072315 0.0 34-35 0.0 0.0 0.0 0.004558915211970075 0.0 36-37 0.0 0.0 0.0 0.006663029925187032 0.0 38-39 0.0 0.0 0.0 0.009858167082294264 0.0 40-41 0.0 0.0 0.0 0.013754675810473814 0.0 42-43 0.0 0.0 0.0 0.01691084788029925 0.0 44-45 0.0 0.0 0.0 0.020183915211970077 0.0 46-47 0.0 0.0 0.0 0.02482076059850374 0.0 48-49 0.0 0.0 0.0 0.02934071072319202 0.0 50-51 0.0 0.0 0.0 0.034717892768079794 0.0 52-53 0.0 0.0 0.0 0.04017300498753117 0.0 54-55 0.0 0.0 0.0 0.04445916458852868 0.0 56-57 0.0 0.0 0.0 0.050031172069825436 0.0 58-59 0.0 0.0 0.0 0.05766832917705736 0.0 60-61 0.0 0.0 0.0 0.0651496259351621 0.0 62-63 0.0 0.0 0.0 0.07157886533665836 0.0 64-65 0.0 0.0 0.0 0.07828086034912718 0.0 66-67 0.0 0.0 0.0 0.08642456359102245 0.0 68-69 0.0 0.0 0.0 0.0954254987531172 0.0 70-71 0.0 0.0 0.0 0.10637468827930174 0.0 72-73 0.0 0.0 0.0 0.11677836658354115 1.5586034912718204E-4 74-75 0.0 0.0 0.0 0.1273768703241895 1.9482543640897756E-4 76-77 0.0 0.0 0.0 0.138715710723192 2.3379052369077306E-4 78-79 0.0 0.0 0.0 0.1510676433915212 2.3379052369077306E-4 80-81 0.0 0.0 0.0 0.1650561097256858 2.3379052369077306E-4 82-83 0.0 0.0 0.0 0.17986284289276808 2.3379052369077306E-4 84-85 0.0 0.0 0.0 0.19404613466334164 2.3379052369077306E-4 86-87 0.0 0.0 0.0 0.2091645885286783 2.3379052369077306E-4 88-89 0.0 0.0 0.0 0.22634819201995013 2.3379052369077306E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1905 0.0 63.90209 1 GTATCAA 3620 0.0 43.611355 1 TCAACGC 4345 0.0 35.41745 4 ATCAACG 4400 0.0 34.974735 3 CAACGCA 4510 0.0 34.121693 5 TATCAAC 4525 0.0 34.008583 2 AACGCAG 4725 0.0 33.271423 6 ACGCAGA 5245 0.0 29.701164 7 CGCAGAG 5330 0.0 29.227509 8 TAACGCT 70 0.008394902 27.140884 4 TACATGG 4325 0.0 25.258284 2 GTACATG 4545 0.0 24.900791 1 GCAGAGT 6270 0.0 24.769964 9 ACATGGG 4350 0.0 23.80287 3 CAGAGTA 5935 0.0 22.687021 10-11 AGAGTAC 5605 0.0 22.328018 10-11 GAGTACT 3935 0.0 22.088612 12-13 CATGGGG 2580 0.0 20.434563 4 GTACTTT 4415 0.0 20.011433 14-15 AGTACTT 4355 0.0 19.522118 12-13 >>END_MODULE