FastQCFastQC Report
Sat 31 Dec 2022
SRR1954866.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954866.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318829
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55621.7445088119336698No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18990.5956170862750879No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC15480.4855267243569437No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC13350.41871975259465105No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG13160.4127604452543526No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT12390.38860956813840647No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC11650.36539963428671796No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC9270.29075146865561163No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.2819693315225403No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT6330.19853902875836263No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC5420.1699970830758808No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC4760.14929633126221267No Hit
ACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC4040.12671369292002924No Hit
GGAAAAGGGAGACTTCAACCCCACAGAGTCCTCTCTTACCACCCCCATATG3620.11354048722042223No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3590.11259954395616459No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT3390.10632658886111364No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3370.10569929335160855No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT3230.10130822478507287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)