FastQCFastQC Report
Sat 31 Dec 2022
SRR1954865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137561
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14881.0817019358684512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9180.6673403072091654No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.31186164683304135No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC3950.28714533915862783No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG3410.2478900269698534No Hit
GCTGTCACTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC3390.2464361265184173No Hit
CCTGTCACTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC2730.19845741162102631No Hit
CTGTCTATTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT2700.19627656094387216No Hit
GAATCTATCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC2560.18609925778381953No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1740.12648933927493985No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.10976948408342481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)