FastQCFastQC Report
Sat 31 Dec 2022
SRR1954864.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954864.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210356
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196511.6235719077692845No Hit
GAATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC68360.5647925073284223No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG42100.3478315470820155No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC27090.22381844680408078No Hit
GAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT24330.20101523849181563No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC16500.13632352795375904TruSeq Adapter, Index 16 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT14260.11781657628003661No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTC13690.11310721804163404No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC12620.10426684380463269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)