FastQCFastQC Report
Sat 31 Dec 2022
SRR1954862.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954862.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394557
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50941.2910682106767843No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG18930.4797785871242939No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC18500.4688802885261191No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC15580.3948732375803749RNA PCR Primer, Index 36 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC11560.2929868181276723RNA PCR Primer, Index 15 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT8860.22455564088331978RNA PCR Primer, Index 36 (96% over 26bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.22404874327410235No Hit
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC8200.2078280197791447RNA PCR Primer, Index 36 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCC6380.16170033734035894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6300.15967274690348923No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACAATCT5600.1419313305808793No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTC4770.12089507979835612No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT4740.12013473338452998No Hit
CAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAA4430.11227782044165989No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTA3950.10011227782044166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)