FastQCFastQC Report
Sat 31 Dec 2022
SRR1954861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159370
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.8746941080504487No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG4400.2760870929284056No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC4220.2647926209449708No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.24785091296981865No Hit
GAATCTATCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC3470.2177323210139926RNA PCR Primer, Index 15 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.1888686703896593No Hit
GCTGTCACTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC2400.15059295977913031RNA PCR Primer, Index 15 (96% over 25bp)
CAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAA1970.12361172115203614No Hit
CTGTCTATTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT1820.1141996611658405RNA PCR Primer, Index 15 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)