FastQCFastQC Report
Sat 31 Dec 2022
SRR1954858.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954858.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370866
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40861.101745643979227No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG24670.6651998295880454No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC23730.6398537477148081No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC23000.6201700883877196No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC8930.2407877777957537TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT8570.2310807677166416No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.19710623243974912No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT7010.18901705737382235TruSeq Adapter, Index 15 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT6830.1841635523342663No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5710.153963965421473No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5460.14722298619986735No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT5370.14479623368008931No Hit
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC5280.14236948116031128TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA5110.13778561528961944No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC4870.13131427523687803No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC4730.12753932687277883No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG4720.1272696877039146No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC3760.10138432749294893No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)