FastQCFastQC Report
Sat 31 Dec 2022
SRR1954857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152754
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.7796849836992812No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC6000.39278840488628775No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG5840.3823140474226534No Hit
GAATCTATCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC5580.3652932165442476No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.23436374824881834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.19901279180905246No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2830.1852651976380324No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC1650.10801681134372913No Hit
GCTGTCACTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC1650.10801681134372913TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG1600.1047435746363434No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC1550.10147033792895768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)