FastQCFastQC Report
Sat 31 Dec 2022
SRR1954856.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954856.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546099
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54901.0053122236078074No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG26680.48855610429610746No Hit
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT23960.43874828556726897TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC21680.3969976139857425TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC21640.3962651460632596No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC20620.3775872140399451No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC19840.3633040895515282No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC17580.3219196519312432TruSeq Adapter, Index 20 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10590.193920882477353No Hit
TCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC10310.18879360701997255TruSeq Adapter, Index 20 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.1670026863261057No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC8920.1633403467136911No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACGATACT8610.15766372031444847No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT8170.14960657316713638No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTC7200.13184422604692556No Hit
ACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC6820.12488578078333783TruSeq Adapter, Index 23 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT6730.12323772795775124No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACGATACTCGTA6490.11884292042285373No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG6250.11444811288795621No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTC6140.11243382610112819No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)