FastQCFastQC Report
Sat 31 Dec 2022
SRR1954855.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954855.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210555
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.7280757996722947No Hit
GAATCTATCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC5980.2840113034599036No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG5580.26501389185723445No Hit
CTGTCTATTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT5500.2612144095367006TruSeq Adapter, Index 23 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5300.25171570373536606No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.21419581582009453No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC4470.21229607465982758No Hit
GCTGTCACTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC4400.2089715276293605TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCACTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC3490.16575241623328824TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCACTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC2260.10733537555508062TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)