FastQCFastQC Report
Sat 31 Dec 2022
SRR1954854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472104
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74921.5869384711843155No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG52151.1046294884178063No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC51341.0874722518767053No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC41560.8803145069730398No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC17410.3687746767661363TruSeq Adapter, Index 19 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT15840.33551929235931066No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT15090.3196329622286615No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGT14960.3168793316726823No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTC14170.30014573060173183No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT14110.29887482419127986TruSeq Adapter, Index 13 (95% over 23bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.2857421246166099No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA12310.2607476318777219No Hit
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC10580.22410316370969108TruSeq Adapter, Index 19 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG9590.2031332079372342No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC8200.17369054276176435No Hit
GAATATGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC8160.17284327182146306No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7790.16500601562367614No Hit
TCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC6870.14551878399674648TruSeq Adapter, Index 19 (95% over 22bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC6370.13492789724298035No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5480.11607611882127668No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGT4860.10294341924660669No Hit
ACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC4840.10251978377645604TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)